Excessive zinc chloride induces murine photoreceptor cell death via reactive oxygen species and mitochondrial signaling pathway

Wang, Hui; Liu, Bin; Yin, Xuewei; Guo, Lijie; Jiang, Wenjun; Bi, Hongsheng; Guo, Dadong

doi:10.1016/j.jinorgbio.2018.07.004

Cited by 15 publications

(6 citation statements)

References 34 publications

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“…In this study, we detected the inhibitory effect of ZnO NPs on the proliferation of human AML cells, and the results showed that ZnO NPs could significantly inhibit cell proliferation and facilitate the apoptosis of human monocytic cell line THP‐1 cells. Our previous studies have found that ZnO NPs can exhibit apparent cytotoxic effect on leukemia cells, 42 rat retinal ganglion cells, 43 and mouse photoreceptor cells at high concentrations, 44 which were consistent with the present findings. Moreover, previous studies also showed that ZnO NPs had no significant toxicity and inhibitory effect on normal human cells 45 …”

Section: Discussionsupporting

confidence: 93%

Induced effect of zinc oxide nanoparticles on human acute myeloid leukemia cell apoptosis by regulating mitochondrial division

Yin

Lyu

et al. 2022

IUBMB Life

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Zinc oxide nanoparticles (ZnO NPs) have exhibited excellent anti‐tumor properties; the present study aimed to elucidate the underlying mechanism of ZnO NPs induced apoptosis in acute myeloid leukemia (AML) cells by regulating mitochondrial division. THP‐1 cells, an AML cell line, were first incubated with different concentrations of ZnO NPs for 24 hr. Next, the expression of Drp‐1, Bcl‐2, Bax mRNA, and protein was detected, and the effects of ZnO NPs on the levels of reactive oxygen species (ROS), mitochondrial membrane potential (Δψm), apoptosis, and ATP generation in THP‐1 cells were measured. Moreover, the effect of Drp‐1 inhibitor Mdivi‐1 and ZnO NPs on THP‐1 cells was also detected. The results showed that the THP‐1 cells survival rate decreased with the increment of ZnO NPs concentration and incubation time in a dose‐ and time‐dependent manner. ZnO NPs can reduce the cell Δψm and ATP levels, induce ROS production, and increase the levels of mitochondrial division and apoptosis. In contrast, the apoptotic level was significantly reduced after intervention of Drp‐1 inhibitor, suggesting that ZnO NPs can induce the apoptosis of THP‐1 cells by regulating mitochondrial division. Overall, ZnO NPs may provide a new basis and idea for treating human acute myeloid leukemia in clinical practice.

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Section: Discussionsupporting

confidence: 93%

Induced effect of zinc oxide nanoparticles on human acute myeloid leukemia cell apoptosis by regulating mitochondrial division

Yin

Lyu

et al. 2022

IUBMB Life

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“…12 For example, high-level Zn 2+ inhibited the proliferation of murine photoreceptor cells, while appropriately low levels of Zn 2+ enhanced cell proliferation. 67 In addition, Zn in photoreceptor cells was mainly found in the OSs, where the availability of Zn depends on light−dark adaptation. 68,69 Developmental Patterns of Photoreceptor Cells of Medaka Eyes via Different Zn Exposure Modes.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Photoreceptor cells are highly sensitive to Zn levels . For example, high-level Zn 2+ inhibited the proliferation of murine photoreceptor cells, while appropriately low levels of Zn 2+ enhanced cell proliferation . In addition, Zn in photoreceptor cells was mainly found in the OSs, where the availability of Zn depends on light–dark adaptation. , …”

Section: Resultsmentioning

confidence: 99%

Meeting Zn Needs during Medaka Eye Development: Nanoscale Visualization of Retina by Expansion Microscopy

Wang

2022

Environ. Sci. Technol.

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Fish eyes require high Zn levels to support their early development. Although numerous studies have been conducted on the nutritional and toxic effects of Zn on the eye, the Zn requirement for retinal cell development is still debatable. Moreover, due to the complexity of the retinal structure, it is difficult to clearly visualize each retinal layer and accurately separate cell morphology in vivo by conventional methods. In the present study, we for the first time have achieved nanoscale imaging of retinal anatomy affected by dietary and waterborne Zn exposure by novel expansion microscopy. We demonstrated that the fish retina showed different developmental strategies in response to dietary and aqueous Zn exposures. Excess dietary Zn produced toxicity to retinal photoreceptor cells, resulting in a reduction in cell number and cell area, and this toxicity became severe with biological development. In contrast, waterborne Zn in the natural environment probably failed to meet the Zn requirements of retinal development. Overall, our results indicated that during early development, the Zn requirement of the fish eyes was sensitive, and oversupplementation led to impaired photoreceptor cell development. Our study has provided new perspectives using the powerful and novel expansion microscopy technique in toxicity assessment, enabling ultra-clear visualization of small but complex organ development.

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“…A normal ΔΨm is necessary for mitochondrial ATP production [ 62 ] and oxidative phosphorylation [ 63 ]. When ΔΨm decreases, mitochondrial permeability increases [ 64 ], ATP synthesis decreases [ 65 ], cytochrome c is released [ 66 ], intracellular oxidative reduction is altered and BCL2 gene family members intervene to accelerate apoptosis [ 67 ]. Importantly, the release of mitochondrial membrane proteins and the dissipation of ΔΨm occur frequently in disease states with increased cell death [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Maslinic Acid Supplementation during the In Vitro Culture Period Ameliorates Early Embryonic Development of Porcine Embryos by Regulating Oxidative Stress

Yang

Sun

et al. 2023

Animals

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As a pentacyclic triterpene, MA exhibits effective free radical scavenging capabilities. The purpose of this study was to explore the effects of MA on porcine early-stage embryonic development, oxidation resistance and mitochondrial function. Our results showed that 1 μM was the optimal concentration of MA, which resulted in dramatically increased blastocyst formation rates and improvement of blastocyst quality of in vitro-derived embryos from parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT). Further analysis indicated that MA supplementation not only significantly decreased the abundance of intracellular reactive oxygen species (ROS) and dramatically increased the abundance of intracellular reductive glutathione (GSH) in porcine early-stage embryos, but also clearly attenuated mitochondrial dysfunction and inhibited apoptosis. Moreover, Western blotting showed that MA supplementation upregulated OCT4 (p < 0.01), SOD1 (p < 0.0001) and CAT (p < 0.05) protein expression in porcine early-stage embryos. Collectively, our data reveal that MA supplementation exerts helpful effects on porcine early embryo development competence via regulation of oxidative stress (OS) and amelioration of mitochondrial function and that MA may be useful for increasing the in vitro production (IVP) efficiency of porcine early-stage embryos.

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Excessive zinc chloride induces murine photoreceptor cell death via reactive oxygen species and mitochondrial signaling pathway

Cited by 15 publications

References 34 publications

Induced effect of zinc oxide nanoparticles on human acute myeloid leukemia cell apoptosis by regulating mitochondrial division

Induced effect of zinc oxide nanoparticles on human acute myeloid leukemia cell apoptosis by regulating mitochondrial division

Meeting Zn Needs during Medaka Eye Development: Nanoscale Visualization of Retina by Expansion Microscopy

Maslinic Acid Supplementation during the In Vitro Culture Period Ameliorates Early Embryonic Development of Porcine Embryos by Regulating Oxidative Stress

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