Excimer-based Activatable Fluorescent Sensor for Sensitive Detection of Alkaline Phosphatase

Fang, Yuan; Yang, Li; Chen, Zhenjuan; Zhang, Jianjian; Ning, Lulu; Yang, Xiao-Feng; Pu, Kanyi

doi:10.1007/s40242-021-1194-3

Cited by 2 publications

(1 citation statement)

References 34 publications

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“…Although substantial efforts have been dedicated to the design and application of activatable H 2 S donors [18][19][20], the visualization and accurate detection of released H 2 S in living systems remains a crucial challenge, owing to the inconvenience and insufficient sensitivity of many traditional analytical technologies, such as electrochemistry [21,22], gas chromatography [23,24], and colorimetry [25,26]. In recent years, fluorescence imaging has attracted attention among researchers because of its high selectivity, high sensitivity, convenience, low invasiveness, and real-time imaging capability [27][28][29][30][31][32][33][34][35][36][37][38][39][40]. Excellent H 2 S release efficiency with long-wavelength fluorescence emission and the ability to visualize H 2 S release are prerequisites for competent H 2 S donors.…”

Section: Introductionmentioning

confidence: 99%

Biothiol-triggered H2S release from a near-infrared fluorescent H2S donor promotes cutaneous wound healing

Zhao

Ding

Ning

et al. 2022

Acta Materia Medica

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Hydrogen sulfide (H2S) plays crucial roles in antioxidation, anti-inflammation, and cytoprotection. Despite substantial progress in the design and synthesis of activatable H2S donors, methods for high-precision detection and imaging of released H2S in living systems have been lacking. In this study, a biothiol-activated near-infrared (NIR) fluorescent H2S donor, PRO-ST, was developed for real-time visualization of H2S release. PRO-ST consists of a dicyanoisophorone-based NIR fluorescence moiety (TCOO), a biothiol-trigger moiety (4-isothiocyanate benzyl alcohol), and a sulfur-source group (thiophosgene). PRO-ST exhibits high NIR fluorescence enhancement (45-fold), outstanding H2S release efficiency (73%), controllable H2S release (60 min), and excellent cell compatibility. These distinctive features enable PRO-ST to be applied in visualizing H2S release in cells, zebrafish, and mice. Moreover, PRO-ST exhibits excellent performance in visualizing real-time anti-inflammation and wound healing enhancement in biological systems, as confirmed by in situ visualization of H2S release. Thus, PRO-ST provides a versatile and effective method to detect and visualize H2S release, elucidate the mechanisms underlying wound healing, or optimize interventional therapy.

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