Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer

Doraiswamy, Anand; Narayan, Roger J.; Lippert, Thomas; Urech, Lukas; Wokaun, Alexander; Nagel, Matthias; Hopp, B.; Dinescu, M.; Modi, R.; Auyeung, R. C. Y.; Chrisey, Douglas B.

doi:10.1016/j.apsusc.2005.07.166

Cited by 133 publications

(76 citation statements)

References 13 publications

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“…In this approach, the material of interest is dissolved or suspended in a liquid, and submitted to LIFT, leading to the deposition of the solution/suspension in the form of microdroplets. A great variety of complex materials has been successfully printed through LIFT: nanoparticle inks for electrodes in transistors [7,8], polymers for chemical sensors [9], biomolecule solutions for biosensors [10][11][12][13], or even living cells for tissue engineering applications [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Film-free laser forward printing of transparent and weakly absorbing liquids

et al. 2010

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A laser-based technique for printing transparent and weakly absorbing liquids is developed. Its principle of operation relies in the tight focusing of short laser pulses inside the liquid and close to its free surface, in such a way that the laser radiation is absorbed in a tiny volume around the beam waist, with practically no absorption in any other location along the beam path. If the absorbed energy overcomes the optical breakdown threshold, a cavitation bubble is generated, and its expansion results in the propulsion of a small fraction of liquid which can be collected on a substrate, leading to the printing of a microdroplet for each laser pulse. The technique does not require the preparation of the liquid in thin film form, and its forward mode of operation imposes no restriction concerning the optical properties of the substrate. These characteristics make it well suited for printing a wide variety of materials of interest in diverse applications. We demonstrate that the film-free laser forward printing technique is capable of printing microdroplets with good resolution, reproducibility and control, and analyze the influence of the main process parameter, laser pulse energy. The mechanisms of liquid printing are also investigated: timeresolved imaging provides a clear picture of the dynamics of liquid transfer which allows understanding the main features observed in the printed droplets.

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Section: Introductionmentioning

confidence: 99%

Film-free laser forward printing of transparent and weakly absorbing liquids

et al. 2010

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“…Doraiswamy et al used triazine polymer as an intermediate absorbing layer and transferred viable B35 neuroblasts using MAPLE. 76,78 After transferring viable B35 neuroblasts onto receiving substrates, the cell viability and proliferation were examined to show that the MAPLE deposition technique, coupled with an intermediate absorbing layer, was acceptable for creating patterns of viable cells at low fluency. Using MAPLE technology, researchers deposited PLGA/PU polymer to fabricate the substrate topography to study cell viability and preferential orientation of oral keratinocyte stem cells.…”

Section: Biomaterials Deposition Using Matrix Assisted Pulsed Lasementioning

confidence: 99%

Laser-assisted biofabrication in tissue engineering and regenerative medicine

et al. 2016

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Controlling the spatial arrangement of biomaterials and living cells provides the foundation for fabricating complex biological systems. Such level of spatial resolution (less than 10 lm) is difficult to be obtained through conventional cell processing techniques, which lack the precision, reproducibility, automation, and speed required for the rapid fabrication of engineered tissue constructs. Recently, laserassisted biofabrication techniques are being intensively developed with the use of computer-aided processes for patterning and assembling both living and nonliving materials with prescribed 2D or 3D organization. In this review, we discuss laser-assisted fabrication methods, including laser tweezers, multi-photon polymerization, laser-induced forward transfer (LIFT), matrix assisted pulsed laser evaporation (MAPLE), and laser ablation as well as their applications in biological science and biomedical engineering. These advanced technologies enable the precise manipulation of in vitro cellular microenvironments and the ability to engineer functional tissue constructs with high complexity and heterogeneity, which serve in regenerative medicine, pharmacology, and basic cell biology studies.

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“…The feasibility of LIFT to print patterns of individual droplets has been extensively demonstrated [8,9,[12][13][14][15][16][17][18], and in addition, the mechanisms responsible for droplet formation have been widely investigated by time resolved imaging studies [19][20][21][22][23]. Moreover, the next step forward with respect to droplet deposition is the printing of defect-free continuous, uniform, and stable lines.…”

Section: Introductionmentioning

confidence: 99%

“…As an alternative to conventional printing techniques, laser-induced forward transfer (LIFT) has been demonstrated to be feasible for printing biomolecules [7][8][9][10][11][12][13], biomolecule structures [14], as well as cells [15], and micro-organisms [16,17]. In LIFT, a laser beam is focused or imaged through a transparent support onto the backside of a metallic or polymer thin film [11,14,16] coated with the material to be transferred.…”

Section: Introductionmentioning

confidence: 99%

Deposition and characterization of lines printed through laser-induced forward transfer

et al. 2012

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The possibility of printing two-dimensional micro patterns of biomolecule solutions is of great interest in many fields of research in biomedicine, from cell-growth and development studies, to the investigation of the mechanisms of communication between cells. Although laser-induced forward transfer (LIFT) has been extensively used to print micrometric droplets of biological solutions, the fabrication of complex patterns depends on the feasibility of the technique to print micron-sized lines of aqueous solutions.In this study we investigate such a possibility through the analysis of the influence of droplet spacing of a water and glycerol solution on the morphology of the features printed by LIFT. We prove that it is indeed possible to print long and uniform continuous lines by controlling the overlap between adjacent droplets. We show how depending on droplet spacing, several printed morphologies are generated, and we offer, in addition, a simple explanation of the observed behavior based on the jetting dynamics characteristic of the LIFT of liquids.

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Excimer laser forward transfer of mammalian cells using a novel triazene absorbing layer

Cited by 133 publications

References 13 publications

Film-free laser forward printing of transparent and weakly absorbing liquids

Film-free laser forward printing of transparent and weakly absorbing liquids

Laser-assisted biofabrication in tissue engineering and regenerative medicine

Deposition and characterization of lines printed through laser-induced forward transfer

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