Excimer laser surface ablation – a review

Reynolds, Anita; Moore, Johnny; Naroo, Shehzad A.; Moore, CB Tara; Shah, Sunil

doi:10.1111/j.1442-9071.2010.02230.x

Cited by 47 publications

(31 citation statements)

References 105 publications

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“…9,10 It is used widely in corneal refractive surgery. 11 Due to its physical principle, the original contour of the tissue surface is retained. However, due to tissue inhomogeneity, laser ablation can produce a rough surface, which can be smoothened by use of masking substances, such as water or hyaluronic acid solutions.…”

Section: Methodsmentioning

confidence: 99%

Enrichment of Retinal Ganglion Cells in Rat Retinal Lysate by Excimer Laser Ablation of the Outer Retina

Oterendorp

Lagrèze

Gutekunst

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.Retinal ganglion cells (RGC) are a relatively small cell population in the retina. This leads to an unfavorable signal-tonoise ratio when analyzing RGC proteins in whole retina lysate. We present a novel technique to obtain RGC-enriched rat retinal lysate by removing the outer retinal layers with an excimer laser before lysation. METHODS.Outer retinal layers were ablated with an excimer laser on flat mounted retinas from adult albino rats. 4 0 6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) nuclear staining was used to assess the ablation efficacy (n ¼ 6). Western blot for layer specific markers (rhodopsin, parvalbumin, b-III-tubulin) was performed to quantify changes in protein composition (n ¼ 7). Excimer-ablated (EX) and fullthickness (FT) retinas 48 hours after optic nerve crush (ONC) were compared regarding the effect on phospho-cAMP response element binding protein (pCREB) and Thy1 levels (n ¼ 5).RESULTS. Area quantification of DAPI-stained retinas showed that 73% 6 4.9% of the ablation area was free of photoreceptor and bipolar cell nuclei. In Western blot, laser ablation led to a significant reduction of the photoreceptor marker rhodopsin and increase of the ganglion cell layer (GCL) marker b-IIItubulin (relative quantity: rhodopsin 0.47 6 0.05, P < 0.0001; b-III-tubulin 2.35 6 0.37, P ¼ 0.02). Changes of pCREB and Thy1 after ONC were significantly different between FT and EX retinas (relative quantity pCREB: FT 1.4 6 0.16, EX 0.78 6 0.07, P ¼ 0.008; Thy1: FT 0.95 6 0.02, EX 0.63 6 0.07, P ¼ 0.006).CONCLUSIONS. We demonstrated that excimer laser ablation of outer retinal layers is feasible, producing RGC-enriched retinal lysate. Laser ablation may allow a more specific detection of RGC responses to experimental stimuli. (Invest Ophthalmol Vis Sci.

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Section: Methodsmentioning

confidence: 99%

Enrichment of Retinal Ganglion Cells in Rat Retinal Lysate by Excimer Laser Ablation of the Outer Retina

Oterendorp

Lagrèze

Gutekunst

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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“…Laser-assisted in situ keratomileusis (LASIK) is the most common laser refractive surgery performed followed by photorefractive keratectomy (PRK) [1,2]. Like any procedure, the risk of infection remains a hazard that can be sightthreatening.…”

Section: Introductionmentioning

confidence: 99%

Post-keratorefractive Surgery Infections: Management Strategies

Pillar

Munir

2015

Curr Ophthalmol Rep

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Infectious keratitis after keratorefractive surgery, although extremely rare, has the potential to be sightthreatening. Key to the management of post-operative bacterial and fungal keratitis is early diagnosis, identification of the causative organism with scrapings, irrigation with clinically appropriate antimicrobials, and subsequent topical antimicrobial drop administration. In non-responsive cases, confocal microscopy or polymerase chain reaction may aid in the identification of the causative organism to guide antimicrobial management.

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“…One to four days after the intervention most of the patients have a transient low-intensity pain. Postoperative visual rehabilitation is a little longer and lasts several weeks (1,15).…”

Section: Photorefractive Keratectomy (Prk)mentioning

confidence: 99%

“…In addition, flat (< 41 D) or steep corneas (> 48 D), with the risk of free, thin, incomplete or buttonholed flaps, may be better suited to PRK. It is desirable to avoid suction and iatrogenically raising the IOP during LASIK, as in patients with glaucoma or a risk of poor optic nerve perfusion, PRK procedure would be preferred (15).…”

Section: Photorefractive Keratectomy (Prk)mentioning

confidence: 99%