2004
DOI: 10.1128/jb.186.16.5551-5554.2004
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Excision of the Shigella Resistance Locus Pathogenicity Island in Shigella flexneri Is Stimulated by a Member of a New Subgroup of Recombination Directionality Factors

Abstract: Pathogenicity islands are capable of excision and insertion within bacterial chromosomes. We describe a protein, Rox, that stimulates excision of the Shigella resistance locus pathogenicity island in Shigella flexneri. Sequence analysis suggests that Rox belongs to a new subfamily of recombination directionality factors, which includes proteins from P4, enterohemorrhagic Escherichia coli, and Yersinia pestis.

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Cited by 16 publications
(15 citation statements)
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“…As shown above both Tyr 28 and Arg 45 proved to be crucial for the excisionase activity of TorI. According to these sequence similarities we thus predict that all of these proteins share the same secondary structures and fold, as well as the same function, which is consistent with the in vivo excisionase role of at least three of them, TorI, Hef, and Rox in E. coli, Y. pseudotuberculosis, and S. flexneri, respectively (14,15). Since TorI is also involved in gene regulation, we propose that this particular family of RDF proteins may be also involved in regulatory processes.…”
Section: Tori the Structural Missing Link Betweensupporting
confidence: 77%
See 1 more Smart Citation
“…As shown above both Tyr 28 and Arg 45 proved to be crucial for the excisionase activity of TorI. According to these sequence similarities we thus predict that all of these proteins share the same secondary structures and fold, as well as the same function, which is consistent with the in vivo excisionase role of at least three of them, TorI, Hef, and Rox in E. coli, Y. pseudotuberculosis, and S. flexneri, respectively (14,15). Since TorI is also involved in gene regulation, we propose that this particular family of RDF proteins may be also involved in regulatory processes.…”
Section: Tori the Structural Missing Link Betweensupporting
confidence: 77%
“…All of these proteins share a small size (less than 80 residues) and a high proportion of basic residues, which are typical characteristics of RDF proteins (2). Indeed, two homologues of TorI have been recently described as pathogenic island excisionases, namely Hef and Rox in Y. pseudotuberculosis, and S. flexneri, respectively (14,15). All of these data led us to suspect a role for TorI in the excision of the KplE1 cryptic prophage in E. coli.…”
mentioning
confidence: 95%
“…rox is believed to encode a recombination directionality factor (RDF) and stimulates excision of the island by an as yet unknown mechanism (Sakellaris et al ., 2004). The best‐studied example of an RDF is the Xis protein of phage λ (Kim and Landy, 1992) and analogous genes have been recently identified in a variety of PAIs (Lesic et al ., 2004; Luck et al ., 2004; Sakellaris et al ., 2004). However, a rox ‐like gene was not identified in the nucleotide sequence of PAI V 536 and PAI VI 536 (Schneider et al ., 2004; E. Brzuszkiewicz et al ., submitted).…”
Section: Discussionmentioning
confidence: 99%
“…The hypothesis that genomic islands could be transmitted by phages has been put forward because, as with bacteriophages, these islands are most often inserted into tRNA genes, they excise from the host chromosome by site-specific recombination between flanking direct repeats homologous to phage attachment sites, and their excision is mediated by a phagelike integrase and sometimes a recombination directionality factor (22,25,36). Pathogenicity islands (PAIs) are a subset of genomic islands characterized by their capacity to encode virulence functions (17).…”
mentioning
confidence: 99%