Excitation-Contraction Coupling in Ureteric Smooth Muscle: Mechanisms Driving Ureteric Peristalsis

Burdyga, Theodor; Lang, Richard J

doi:10.1007/978-981-13-5895-1_4

Cited by 7 publications

(4 citation statements)

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“…4A). It is known that the slowing down of the intensification of the force of spontaneous phase contractions in visceral SM is mainly conditioned by suppressing the intake of Са 2+ ions to SMC from the extracellular space via potential-governed Са 2+ -channels [2,7]. Thus, it can be inferred that under preincubation of myometrium preparations with calix [4]arene C-90, there process of the intake of this cation via the plasma membrane is suppressed; this effect may cause a general decrease in the activity index of uterine contractions.…”

Section: Resultsmentioning

confidence: 99%

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Modulating the mechanokinetics of spontaneous contractions of the myometrium of rats using calix[4]arene C-90 – plasma membrane calcium ATPase inhibitor

Tsymbalyuk¹

2021

Biol. Stud.

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Introduction. Plasma membrane calcium ATPase is a constitutive structure of cells that functions as a high affinity system of releasing Са2+ ions from the cytoplasm and ensures a long-term maintenance of the basal concentration of these cations in the state of dormancy. Currently, there are no satisfactory means for the pharmacological correction of plasma membrane calcium ATPase function. Thus, elaboration, synthesis, and study of substances with the targeted impact on plasma membrane calcium ATPase are topical issues. Previously, we determined the ability of this calix[4]arene in the concentration of 10 µM to inhibit the contractive activity and to slow down the relaxation of smooth muscle of the myometrium in the NO-dependent way, which considerably decreases the normalized maximal velocity of the relaxation phase. Materials and Methods. The tenzometric methods and mechanokinetic analysis were used to investigate the impact of the cumulative increase in the concentration of calix[4]arene С-90 (10 nM – 100 µM) on the spontaneous contractive activity of the myometrium of rats. The complete profile of spontaneous cycles of contractions-relaxations was studied by the empirical multiparameter method of complex mechanokinetic analysis, elaborated by us (with the consideration of the parameters of time (τ0, τC and τR), force (Fmax, FC and FR), velocity (VC and VR), and impulse (Іmax, ІC and ІR). Results. Calix[4]arene C-90 evoked the dose-dependent inhibition of spontaneous contractive activity of the myometrium preparations. Its high concentrations caused a change in the structure of the contraction act, such as an increase in the duration of the contraction phase, while the duration of the relaxation phase did not show any changes. The multiparameter method of the complex mechanokinetic analysis demonstrated that in the whole range of the investigated concentrations, substance С-90 considerably decreases the indices of force parameters (Fmax, FC and FR) and the values of impulses of force Іmax, IC and IR of the spontaneous contractions of the myometrium. On the background of all the applied concentrations (10-7–10-4 М), calix[4]arene С-90 conditioned the slowing down of the relaxation of spontaneous contractions in uterine muscle preparations of rats, which was reflected in a reliable decrease in the parameter for the maximal velocity of the relaxation phase (VR). Conclusions. The results of the study demonstrate that calix[4]arene С-90 inhibits the processes of Са2+ extrusion from smooth muscle cells myoplasm, probably, impacting plasma membrane calcium ATPase molecules directly. It is noteworthy that C-90 is also likely to inhibit the processes of the intake of these cations to cells from the extracellular medium, causing a decrease in the velocity of force intensification during the contraction phase, and reducing the frequency and force of the spontaneous contractions in the myometrium.

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Section: Resultsmentioning

confidence: 99%

“…The stoichiometry of the transported Ca 2+ ions and hydrolyzed ATP molecules is 1:1. Four isoforms of PMCA (1)(2)(3)(4) were identified, which are encoded by different genes; the additional diversity (over 20) of isoforms is ensured by the alternative splicing [6].…”

Section: Introductionmentioning

confidence: 99%

Modulating the mechanokinetics of spontaneous contractions of the myometrium of rats using calix[4]arene C-90 – plasma membrane calcium ATPase inhibitor

Tsymbalyuk¹

2021

Biol. Stud.

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“…(8) A meta-analysis by the AUA guidelines panel determined that ureteral stones with a diameter of <5 mm will pass in up to 98% of cases. For stones with diameters >7 mm, the overall chance of spontaneous passage is low (9)(10)(11). A wide range of spontaneous passage rates have been reported, ranging from 71% to 98% for distal ureteral stones <5 mm and 25%-53% for stones measuring 5-10 mm with a mean expulsion time of >10 days and is associated with high analgesic requirement even for stones <5 mm.…”

Section: Discussionmentioning

confidence: 99%

Tadalafil versus Tamsulosin as a medical expulsive therapyfor solitary unilateral lower ureteric stone less than 1 cm: a prospective randomized study.

Rahim

Abdel-Raheum

Sayed

et al. 2020

Minia Journal of Medical Research

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Introduction:In up to 50 percent of cases, expectant treatment may result in spontaneous stone expulsion. The therapeutic potential of α-adrenergic blockers for ureteral stone disease was investigated, prompted by α-receptor detection in ureteral smooth muscle cells improves the passage of larger ureteric stones (5-10 mm).A newly launched phosphodiesterase-5 (PDE-5) inhibitor, tadalafil, has emerged that acts on the smooth muscle NO / cGMP and can be used as expulsive therapy. Aim of the work: To assess the impact of Tadalafil vs. Tamsulosin, as medical expulsive therapy of solitary unilateral lower ureteric stone less than 1 cm. Patients and Methods: Our prospective, randomized, placebo-controlled, comparative clinical study at Nephrology and Urology Minia University Hospital, included 99 patients who randomized into 3 equal groups, 33 patients in each group.First group received Tadalafil 5 mg, second group received Tamsulosin 0.4mg, third group was control group (placebo). Results: The stone expulsion rate was 78.8%, 81.8% and 54.5% respectively, with a significant difference between group (A,B) and group (C). Mean days till stone passed was (11.17±5.1), (10.27±4.17) and (15.7±3.66) respectively, the difference is statistically significant.

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“…The second messenger Ca 2+ activates the Ca 2+ /calmodulin-dependent myosin light chain kinase-dependent phosphorylation of 20-kDa regulatory light chains of myosin, which leads to ureteric contraction. In ammatory factors can initiate spontaneous activity in the proximal and distal ureter (16).…”

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confidence: 99%

Influence of Febrile Urinary Tract Infection on Ultrasonic Measurements of Nonreflux Upper Urinary Tract Dilation in Infants

Liu

Huang

2021

Preprint

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Background To investigate the changes in ultrasonic measurements of nonreflux upper urinary tract dilation in infants with febrile urinary tract infection (UTI).Methods There were 28 cases of nonreflux upper urinary tract dilatation with febrile UTI: 14 cases of ureteropelvic junction obstruction (UPJO) (14 kidneys) and 14 cases of ureterovesical junction obstruction (UVJO) (16 kidneys). Changes in anteroposterior renal pelvic diameter (APD) and ureteral dilatation during infection and after infection were compared in UPJO and UVJO patients, respectively.Results In the UPJO with febrile UTI group, the APD was 24.1±10.0 mm at the time of UTI and 16.6±7.0 mm 1 week after infection recovery (P<0.001). In the UVJO with febrile UTI group, the APD was 19.3±8.5 mm at the time of UTI and 15.2±7.7 mm 1 week after infection recovery (P<0.001). In the UVJO with febrile UTI group, the ureteric diameter was 11.0±3.2 mm during UTI and 6.8±2.6 mm 1 week after infection recovery (P<0.001).Conclusions In UPJO patients, the APD decreased after febrile UTI treatment compared with that during infection. In UVJO patients, the APD and ureteric diameter decreased after febrile UTI treatment compared with that during infection.

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Excitation-Contraction Coupling in Ureteric Smooth Muscle: Mechanisms Driving Ureteric Peristalsis

Cited by 7 publications

References 89 publications

Modulating the mechanokinetics of spontaneous contractions of the myometrium of rats using calix[4]arene C-90 – plasma membrane calcium ATPase inhibitor

Modulating the mechanokinetics of spontaneous contractions of the myometrium of rats using calix[4]arene C-90 – plasma membrane calcium ATPase inhibitor

Tadalafil versus Tamsulosin as a medical expulsive therapyfor solitary unilateral lower ureteric stone less than 1 cm: a prospective randomized study.

Influence of Febrile Urinary Tract Infection on Ultrasonic Measurements of Nonreflux Upper Urinary Tract Dilation in Infants

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