Excitation Energy Transfer and the Quantitative Study of the Antibody Hapten Reaction

Velick, Sidney F.; Parker, Charles W.; Eisen, Herman N.

doi:10.1073/pnas.46.11.1470

Cited by 162 publications

(66 citation statements)

References 5 publications

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“…Despite the theoretical difficulties discussed above the differences between the values obtained by the two methods of measurement are small and consistent and the fluorescencequenching technique has been empirically justified both in the present paper and by previous workers (28,29). It can be regarded as capable of giving accurate association constants for the antibody-hapten system studied.…”

Section: Measurement Of Antibody-binding A~inity By Fluorescence Quesupporting

confidence: 64%

“…In the course of the present work the method of fluorescence quenching (28,29) has been applied to the measurement of affinity of guinea pig antibodies specific for the e-2,4-dinitrophenyl-L-lysyl determinant. The method was calibrated by comparison with equilibrium dialysis.…”

Section: Discussionmentioning

confidence: 99%

“…anti-DNP antibodies for ¢-DNP-I,-lysine were calculated from the quenching of antibody fluorescence upon binding of hapten (28). The technique of titration and method of calculation of average association constants from the data obtained have been described in detail by Eisen and Siskind (29) for rabbit anti-DNP antibody.…”

Section: Measurement Of Antibody-binding A~inity By Fluorescence Quementioning

confidence: 99%

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Studies on the Effect of the Carrier Molecule on Antihapten Antibody Synthesis

Siskind

Paul

Benacerraf

1966

The Journal of Experimental Medicine

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The carrier to which a hapten is coupled can influence both the nature and the immunological specificity of the immune response to the hapten. Thus, guinea pigs genetically capable of an immune response to haptens attached to the T.-lysine homopolymer (1, 2) fail to respond to the same haptens coupled to poly-D-lysine (3, 4). Evidence for definite "carrier specificity," that is, immunologically specific interactions involving portions of the carrier molecule adjacent to the attached hapten, has been clearly demonstrated with regard to the ability of hapten-protein conjugates to stimulate a secondary response in rabbits (5) or to elicit delayed hypersensitivity reactions in sensitized guinea pigs (6, 7). In addition, various degrees of carrier specificity have also been observed for several antigen-antibody reactions: wheal and flair reactions in man (8), passive cutaneous anaphylaxis in guinea pigs with early immunization sera (9), some Arthus reactions (10), and precipitin reactions in liquid media in selected systems (11-13).The present studies were undertaken to define, in quantitative terms, the effect of the carrier upon the antibody response to the 2,4-dinitrophenyl (DNP) determinant. In this paper, the effect of varying the carrier molecule upon the immunoglobulin class, the amount, and the average intrinsic association constant (K0) of the antibody produced, has been investigated in the guinea pig. In a second paper (14), the specificity of guinea pig anti-2,4-dinitrophenylpoly-L-lysine antibodies for the poly-L-lysine carrier is demonstrated and the amount of energy contributed to the antibody-antigen interaction by specific binding of the carrier molecule is estimated.

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Section: Measurement Of Antibody-binding A~inity By Fluorescence Quesupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Measurement Of Antibody-binding A~inity By Fluorescence Quementioning

confidence: 99%

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Studies on the Effect of the Carrier Molecule on Antihapten Antibody Synthesis

Siskind

Paul

Benacerraf

1966

The Journal of Experimental Medicine

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“…In the derivation of equations (2) (Berson & Yalow, 1958), e-N-dinitrophenyl-lysine (Velick, Parker & Eisen, 1960) and fluorescein-ovalbumin (Dandliker & Feigen, 1961) and their respective antibodies.…”

Section: Vol 85 471 Discussionmentioning

confidence: 99%

The kinetics of the reaction between the blood-group antibody anti-c and erythrocytes

Hughes‐Jones¹,

Gardner²,

Telford³

1962

Biochemical Journal

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“…Subsequently, Velick, together with Parker and Eisen (Velick et al, 1960), utilized the Aminco-Bowman spectrophotofluorometer to investigate antigen-antibody interactions.…”

Section: Monochromator Calibrated Wavelength P O T E~t I O M E T E~ mentioning

confidence: 99%

Development of the spectrophotofluorometer and its commercialization

Udenfriend¹

1995

Protein Science

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Excitation Energy Transfer and the Quantitative Study of the Antibody Hapten Reaction

Cited by 162 publications

References 5 publications

Studies on the Effect of the Carrier Molecule on Antihapten Antibody Synthesis

Studies on the Effect of the Carrier Molecule on Antihapten Antibody Synthesis

The kinetics of the reaction between the blood-group antibody anti-c and erythrocytes

Development of the spectrophotofluorometer and its commercialization

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