Excitatory Action of Synthetic Prostaglandin E2 on the Electrical Activity of Pregnant Mouse Myometrium in Relation to Temperature Changes and External Sodium and Calcium Concentrations

Osa, Takuro; Suzuki, Hiroyoshi; Katase, Takashi; Kuriyama, Hirosi

doi:10.2170/jjphysiol.24.233

Cited by 19 publications

(9 citation statements)

References 20 publications

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“…The recordings showed that the electrical activity in the smooth muscle was composed of intermittent bursts of spike‐like action potentials (Marshall , Osa et al . , Kuriyama & Suzuki ). The single spikes could initiate brief submaximal phasic contractions, but bursts of multiple spikes were needed for the generation of strong long‐lasting contractions with plateaus (Marshall , Coleman et al .…”

Section: Electrophysiological Studies Of Myometriummentioning

confidence: 94%

“…When successful, these impalements recorded the changes in electrical potential between the inside and outside of the cell and showed the spontaneous changes in this potential, due to the myogenic nature of the myometrium. The recordings showed that the electrical activity in the smooth muscle was composed of intermittent bursts of spike-like action potentials (Marshall 1962, Osa et al 1974, Kuriyama & Suzuki 1976. The single spikes could initiate brief submaximal phasic contractions, but bursts of multiple spikes were needed for the generation of strong long-lasting contractions with plateaus (Marshall 1962, Coleman et al 2000.…”

Section: Electrophysiological Studies Of Myometriummentioning

confidence: 99%

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Progress in understanding electro-mechanical signalling in the myometrium

et al. 2014

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In this review, we give a state-of-the-art account of uterine contractility, focussing on excitation-contraction (electro-mechanical) coupling (ECC). This will show how electrophysiological data and intracellular calcium measurements can be related to more modern techniques such as confocal microscopy and molecular biology, to advance our understanding of mechanical output and its modulation in the smooth muscle of the uterus, the myometrium. This new knowledge and understanding, for example concerning the role of the sarcoplasmic reticulum (SR), or stretch-activated K channels, when linked to biochemical and molecular pathways, provides a clearer and better informed basis for the development of new drugs and targets. These are urgently needed to combat dysfunctions in excitation-contraction coupling that are clinically challenging, such as preterm labour, slow to progress labours and post-partum haemorrhage. It remains the case that scientific progress still needs to be made in areas such as pacemaking and understanding interactions between the uterine environment and ion channel activity.

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Section: Electrophysiological Studies Of Myometriummentioning

confidence: 94%

Section: Electrophysiological Studies Of Myometriummentioning

confidence: 99%

Progress in understanding electro-mechanical signalling in the myometrium

et al. 2014

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“…Exogenously applied prostaglandins (mostly E and F series) depolarize the uterine smooth muscle cells of pregnant mouse and rat (Osa, Suzuki, Katase & Kuriyama, 1974;Suzuki & Kuriyama, 1975a, b;Kuriyama & Suzuki, 1976b). Involvement of endogenous prostaglandins in controlling the electrical and mechanical properties of uterus has also been demonstrated (Anderson, Kawarabayashi & Marshall, 1981).…”

Section: Atp-induced Depolarization Of the Membranementioning

confidence: 99%

“…showed potent excitatory actions to the pregnant mouse uterus (Osa, Suzuki, Katase & Kuriyama, 1974;Suzuki & Kuriyama, 1975 a, b). These observations suggest that endogenous prostaglandins probably do not contribute to the ATP-induced depolarization of the mouse myometrium.…”

Section: Atp-induced Depolarization Of the Membranementioning

confidence: 99%

Electrical responses of smooth muscle cells of the mouse uterus to adenosine triphosphate.

Ninomiya¹,

Suzuki²

1983

The Journal of Physiology

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SUMMARY1. Electrical responses of the smooth muscle cells to ATP were recorded in the longitudinal muscle of mouse myometrium, using intracellular micro-electrodes.2. ATP (> 10-7 M) dose-dependently produced a biphasic change in the membrane potential, an initial hyperpolarization (20-30 see) and then a depolarization. This effect of ATP was observed in all stages of gestation. The initial hyperpolarization was more quickly desensitized than the depolarization.3. Application of ATP for a short period (10 see) produced only the initial hyperpolarization; the amplitude was dose-dependently increased.4. During the ATP-induced hyperpolarization and the depolarization, generation of spike potentials was suppressed and enhanced, respectively. Strong outward current restored the spike generation during hyperpolarization.5. During the ATP-induced hyperpolarization, the membrane resistance was decreased. The amplitude of the hyperpolarization was increased in low [K]o solution and decreased in high [K]o solutions.6. Pre-treatment with TEA (1 mM), procaine (1 mM), 4-aminopyridine (0 5 mM) or apamin (2 x 10-7 M) did not, but TEA (5-10 mM) did suppress the ATP-induced hyperpolarization. Involvement of endogenous catecholamines, cyclic AMP, prostaglandins or acetylcholine in the ATP responses was ruled out.7. During the ATP-induced depolarization, the membrane resistance was reduced. In low [Na]0 solutions, the muscle membrane was depolarized and the amplitude of ATP-induced depolarization was reduced. In sodium-free solution, ATP produced only the initial hyperpolarization.8. It was concluded that the electrical responses ofthe smooth muscle cells of mouse myometrium to ATP consist of two components: an initial hyperpolarization with increase in the potassium conductance and a depolarization with increase in the sodium conductance.

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“…Attempts to induce labor with prostaglandins (PGs) have been made in some mammals [1,3,10,[13][14][15] including man [4,6] and monkeys [5,12]. In most of the studies in primates, intrauterine pressure recording was used as an indicator of uterine activity.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Prostaglandins on the Electromyogram and Motility of the Monkey Uterus and Oviduct in Late Pregnancy and Postpartum

Oshima¹,

Takenaka²

1977

J Med Primatol

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The electromyogram of the monkey (Macaca fuscata fuscata and Macaco mulatta) uterus in labor presents spike bursts which accompany the rising intrauterine pressure. Asynchronous localized electrical activity develops into synchronous activity during the course of labor. The early postpartum uterus shows an irregular pattern or continuous small bursts of spikes. During labor it responds markedly to prostaglandins (PGs); this declines in postpartum. Direct intrauterine injection of PGF2a is effective in the early postpartum. Intravaginal application of PGE2 (5 mg/kg) in late pregnancy induces regular, frequent bursts of spikes and successful delivery. Electrical activity of the oviduct was not affected by PGs except in early postpartum.

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Excitatory Action of Synthetic Prostaglandin E2 on the Electrical Activity of Pregnant Mouse Myometrium in Relation to Temperature Changes and External Sodium and Calcium Concentrations

Abstract: Influences of ionsand temperature on the excitatory action

Cited by 19 publications

References 20 publications

Progress in understanding electro-mechanical signalling in the myometrium

Progress in understanding electro-mechanical signalling in the myometrium

Electrical responses of smooth muscle cells of the mouse uterus to adenosine triphosphate.

The Effect of Prostaglandins on the Electromyogram and Motility of the Monkey Uterus and Oviduct in Late Pregnancy and Postpartum

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