Excitatory synaptic transmission in hippocampal area CA1 is enhanced then reduced as chronic epilepsy progresses

Owen, Benjamin; Bichler, Edyta K.; Benveniste, Morris

doi:10.1016/j.nbd.2021.105343

Cited by 9 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of miniature excitatory postsynaptic currents (mEPSCs) was performed to observe neuronal synaptic transmission. 40 After being anesthetized by isoflurane, mice were subjected to perfusion with an oxygenated (95% O 2 /5% CO 2 ) cutting solution consisting of the following reagents: 2.5 mM KCl, 120 mM choline chloride, 7 mM MgCl 2 , 1.25 mM NaH 2 PO 4 , 0.5 mM CaCl 2 , 5 mM sodium ascorbate, 26 mM NaHCO 3 , 3 mM sodium pyruvate, and 25 mM glucose. Then, brains were prepared into 300 μm hippocampal slices using a VT1200S Vibratome (Leica Microsystems).…”

Section: Methodsmentioning

confidence: 99%

Reprogramming astrocytic NDRG2/NF-κB/C3 signaling restores the diabetes-associated cognitive dysfunction

Jiang

et al. 2023

eBioMedicine

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Reprogramming astrocytic NDRG2/NF-κB/C3 signaling restores the diabetes-associated cognitive dysfunction

Jiang

et al. 2023

eBioMedicine

View full text Add to dashboard Cite

“…Animals were divided into five groups ( n = 6 in each group); [ 1 ] control group (sterile normal saline; 0.9% w/v NaCl), [ 2 ] KA-treated group (KA-group), [ 3 ] 40 mg/kg RUF-treated group (KA + low-RUF group), [ 4 ] 80 mg/kg RUF-treated group (KA + medium-RUF group), and [ 5 ] 120 mg/kg RUF-treated group (KA + high-RUF group). During the experiment, one mouse died after KA injection.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, KA is often used to induce recurrent animal models of spontaneous epilepsy. In addition, since the subzone of the hippocampal area is vulnerable to damage induced by KA, the KA-induced disease model is widely applied in the related scientific research of neurodegenerative diseases, such as epilepsy [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Rufinamide (RUF) suppresses inflammation and maintains the integrity of the blood–brain barrier during kainic acid-induced brain damage

Han

et al. 2021

Open Life Sciences

View full text Add to dashboard Cite

Rufinamide (RUF) is a structurally unique anti-epileptic drug, but its protective mechanism against brain injury remains unclear. In the present study, we validated how the RUF protected mice with kainic acid (KA)-induced neuronal damage. To achieve that, a mouse epilepsy model was established by KA intraperitoneal injection. After Nissl staining, although there was a significant reduction in Nissl bodies in mice treated with KA, 40, 80, and 120 mg/kg, RUF significantly reduced KA-induced neuronal damage, in a dose-dependent manner. Among them, 120 mg/kg RUF was most pronounced. Immunohistochemistry (IHC) and western blot analysis showed that RUF inhibited the IBA-1 overexpression caused by KA to block microglia cell overactivation. Further, RUF treatment partially reversed neuroinflammatory cytokine (IL-1β, TNFα, HMGB1, and NLRP3) overexpression in mRNA and protein levels in KA mice. Moreover, although KA stimulation inhibited the expression of tight junctions, RUF treatment significantly upregulated expression of tight junction proteins (occludin and claudin 5) in both mRNA and protein levels in the brain tissues of KA mice. RUF inhibited the overactivation of microglia, suppressed the neuroinflammatory response, and reduced the destruction of blood–brain barrier, thereby alleviating the excitatory nerve damage of the KA-mice.

show abstract

“…In particular, we identified some GO terms related to the synaptic transmission. Dysfunction in the regulation of synaptic transmission plays a major role in epileptogenesis [26], especially in the CA1 subregion [27] which is the biological material analyzed in the GSE136913 dataset. In this context, similarly to the hippocampal subregion comparisons, finding biological processes related to calcium ion ("positive regulation of calcium ion-dependent exocytosis") also makes sense.…”

Section: Biological Interpretation Of Differential Expression Analysismentioning

confidence: 99%

Rattus norvegicus reference genome evaluation for hippocampus RNA-seq data analysis

Priol

Delahaye‐Duriez

2023

Preprint

View full text Add to dashboard Cite

Background:One of the first steps of a usual RNA-seq data analysis workflow consists in quantifying gene expression by aligning the sequencing reads to a reference genome and counting the aligned reads in its annotated regions. Downstream analysis, such as the identification of differentially expressed genes, strongly rely on the quality of this process. In addition to the performance of alignment methods, the choice of reference genome, when several of them are available, may strongly impact this step.Results:Here, we propose to evaluate the effect of widely used \Rn reference genomes consisting of Ensembl and RefSeq annotations of the Rnor\_6.0 assembly and the ones based on the mRatBN7.2 genome assembly recently published by RefSeq, on a classical differential expression workflow. We re-analyzed published RNA-seq datasets from different hippocampal subregions and revealed that the Ensembl and Refseq reference genome based on the mRatBN7.2 assembly provide an improvement of read mapping statistics. We showed that the RefSeq annotations of this assembly make gene expression quantification and differentially expressed gene identification more reliable thanks to overall longer exon length in comparison with Ensembl annotations. Moreover, we identified specific biologically relevant results using RefSeq annotations of the new genome assembly.Conclusion:Overall, the biological interpretation of the differential expression analysis of the analyzed datasets may be dramatically impacted by the choice of reference genome. Therefore, we believe that this choice should be more carefully addressed and that our approach could extend to other tissues and species.

show abstract

Excitatory synaptic transmission in hippocampal area CA1 is enhanced then reduced as chronic epilepsy progresses

Cited by 9 publications

References 40 publications

Reprogramming astrocytic NDRG2/NF-κB/C3 signaling restores the diabetes-associated cognitive dysfunction

Reprogramming astrocytic NDRG2/NF-κB/C3 signaling restores the diabetes-associated cognitive dysfunction

Rufinamide (RUF) suppresses inflammation and maintains the integrity of the blood–brain barrier during kainic acid-induced brain damage

Rattus norvegicus reference genome evaluation for hippocampus RNA-seq data analysis

Contact Info

Product

Resources

About