Exclusion of host cells during spheroid formation from disaggregated solid tumours

Pr, Twentyman

doi:10.1038/bjc.1983.85

Cited by 8 publications

(6 citation statements)

References 6 publications

(10 reference statements)

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“…In addition to cell lines of rodent origin, a great number of human cell lines have been grown as spheroids. The growth characteristics and morphology of these human spheroids have been examined in detail (Carlsson et al, 1983).Recently the spheroid culture system has been approached from the clinical side, by growing as spheroids human cells from solid tumours in immunodepnved mice (Jones et al, 1982;Twentyman, 1983) or human cells directly from surgical specimens (Darling et al, 1983). Darling and coworkers confined their studies to brain tumour cells.…”

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Multicellular spheroids grown directly from human tumour material

Wibe

Berg

Nesland

et al. 1984

Intl Journal of Cancer

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Human tumour cells from surgical material were grown as multicellular spheroids. In 16 out of 20 cultures spheroids with a diameter of more than 250 microns could be observed. In 6 out of 20 cultures more than 30 spheroids with a diameter of at least 300 microns were obtained, i.e. 30% of the cultures fulfilled the criterion for a possible chemosensitivity test on primary cell spheroids. A study of stained sections from the spheroids and the respective tumours, showed that the morphology of the spheroid was very similar that of the tumour from which the cells were derived. Samples from 9 malignant melanomas, 4 bladder carcinomas, 2 renal-cell carcinomas, 2 ovarian carcinomas, 1 lymphoma, 1 colon carcinoma, and 1 schwannoma were tested for spheroid growth. Spheroids were obtained from at least one representative of all these tumour types. However, investigations involving larger numbers of tumours are needed to find out which tumour types are most suitable for further biological characterization of primary cell spheroids and tests for therapy response.

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“…Recently the spheroid culture system has been approached from the clinical side, by growing as spheroids human cells from solid tumours in immunodepnved mice (Jones et al, 1982;Twentyman, 1983) or human cells directly from surgical specimens (Darling et al, 1983). Darling and coworkers confined their studies to brain tumour cells.…”

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confidence: 99%

Multicellular spheroids grown directly from human tumour material

Wibe

Berg

Nesland

et al. 1984

Intl Journal of Cancer

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“…MCTS have been widely used as a model system for solid turnours (Sutherland, 1988). Despite a variety of methods for developing MCTS, little information is available regarding the possible study of tumour-stroma interaction in MCTS established from tumour cells co-cultured with fibroblasts (Twentyman, 1983;Sasaki et at., 1984). At P4 (when fibroblasts were present in the culture), MCTS of HOK-1 appeared to be more tightly packed than MCTS grown from cells of passage 31 (no fibroblasts).…”

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confidence: 99%

Characterization of the new bladder cancer cell line HOK‐I: Expression of transitional, squamous and glandular differentiation patterns

Offner¹,

Ott²,

Povey³

et al. 1991

Intl Journal of Cancer

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The new continuous cell line HOK-1 derived from a grade-III transitional-cell bladder carcinoma with foci of squamous and glandular differentiation was shown to retain this phenotypical heterogeneity for more than 45 passages in vitro. Electron microscopy revealed transitional as well as a considerable proportion of squamous carcinoma and adenocarcinoma cells. PAS-positive mucus was detected in numerous cells. These features were principally maintained when grown as multicellular spheroids and in nude mice. More pronounced signs of differentiation (i.e., expression of cytokeratins 10 and 11, formation of glandular structures) were found in xenograft tumours. Independently, cytokeratins 13, 18 and 19 were detected in vitro and in vivo, reflecting the urothelial origin. The line forms distinct colonies in soft agar, expresses Lewis-x and Lewis-y antigens and reacts with monoclonal antibodies (MAbs) against CEA, beta-HCG and URO-5. Cytogenetic analysis revealed several related clones with a rearrangement at chromosome 1 and loss of one X chromosome as common karyotypic changes in all clones. DNA content, as quantified by image analysis, showed a DNA stemline close to 2c. The new cell line HOK-1 can be used as an in vitro model to study the mechanisms of heterogeneous differentiation patterns in bladder cancer.

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“…Generally, spheroids have been initiated from animal or human cell lines established as monolayer cultures Carlsson et al, 1983) or from disaggregated human tumour xenografts (Jones et al, 1982;Twentyman, 1983;West et al, 1984;Rofstad et al, 1986a). Recently, there has been some interest in growing spheroids directly from human tumour surgical specimens (Darling et al, 1983;Wibe et al, 1984).…”

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Growth and radiosensitivity of malignant melanoma multicellular spheroids initiated directly from surgical specimens of tumours in man

Rofstad¹

1987

Br J Cancer

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Summary The growth and radiosensitivity of multicellular spheroids initiated directly from disaggregated surgical specimens of four human malignant melanomas were studied. The spheroids were grown in liquidoverlay culture for up to 6 passages. Cell survival following irradiation was measured by using the Courtenay soft agar colony assay. The four melanomas formed spherical, densely packed spheroids. The volumetric growth rate as well as the plating efficiency in soft agar usually increased with increasing passage number. The radiosensitivity differed significantly among the melanomas. The survival curves for single cells from disaggregated spheroids in the first passage were always similar to those for single cells isolated directly from the surgical specimens. Two of the melanomas showed a significant contact effect as spheroids whereas the other two did not. The spheroids of two of the melanomas showed lower Do in the third and the sixth passage than in the first passage, whereas the spheroids of the other two melanomas showed similar survival curves in the first and the third passage. There was no clear relationship between the changes in radiosensitivity and the changes in growth rate or plating efficiency. It is concluded that spheroids in the first passage, but not spheroids in later passages, may have the potential to identify differences in clinical radioresponsiveness among tumours.

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Exclusion of host cells during spheroid formation from disaggregated solid tumours

Cited by 8 publications

References 6 publications

Multicellular spheroids grown directly from human tumour material

Multicellular spheroids grown directly from human tumour material

Characterization of the new bladder cancer cell line HOK‐I: Expression of transitional, squamous and glandular differentiation patterns

Growth and radiosensitivity of malignant melanoma multicellular spheroids initiated directly from surgical specimens of tumours in man

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