Exclusive quantification of methyl-branched fatty acids and minor 18:1-isomers in foodstuff by GC/MS in the SIM mode using 10,11-dichloroundecanoic acid and fatty acid ethyl esters as internal standards

Thurnhofer, Saskia; Lehnert, Katja; Vetter, Walter

doi:10.1007/s00217-007-0619-7

Cited by 56 publications

(71 citation statements)

References 31 publications

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“…A 37-component fatty acid methyl ester (FAME) mix for structural assignment, myristic acid (14:0), and pyridine (purity >99.8 %) were from Sigma-Aldrich (Steinheim, Germany). The recovery and syringe standards 10,11-dichloroundecanoic acid (10,11-DC) and myristic acid ethyl ester (14:0-EE) were synthesized as shown elsewhere [23]. The trimethylsilylating reagent which consisted of 99 % N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1 % trimethylchlorosilane (TMCS) was from Supelco (Bellefonte, PA, USA).…”

Section: Experimental Chemicalsmentioning

confidence: 99%

Overcoming the equivalent-chain-length rule with pH-zone-refining countercurrent chromatography for the preparative separation of fatty acids

Englert

Vetter

2015

Anal Bioanal Chem

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Purification of individual fatty acids from vegetable oils by preparative liquid chromatography techniques such as countercurrent chromatography (CCC) is a challenging task due to the equivalent-chain-length (ECL) rule. It implies that one double bond equals two carbon atoms in the alkyl chain of a fatty acid and therefore causes co-elutions of saturated and unsaturated fatty acids. Accordingly, existing methods for the purification of individual fatty acids are cumbersome and time-consuming as two or more steps with different conditions are required. To avoid additional purification steps, we report a method utilizing pH-zone-refining CCC which enabled the purification of all major fatty acids from sunflower oil (purities >95 %) in one step by circumventing co-elutions caused by the ECL rule. This method is based on the involvement of acid strength and hydrophobicity of fatty acids during the separation process. By exploiting the preparative character of the pH-zone-refining mode, a tenfold sample amount of free fatty acids from sunflower oil could be separated in comparison to regular CCC.

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Section: Experimental Chemicalsmentioning

confidence: 99%

Overcoming the equivalent-chain-length rule with pH-zone-refining countercurrent chromatography for the preparative separation of fatty acids

Englert

Vetter

2015

Anal Bioanal Chem

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“…In addition, we recorded the molecular ions [M] þ for the identification of the saturated FAME as well as [M-74] þ and [M-32] þ for the peak assignment and quantification of methyl esters of MUFA according to Hauff and Vetter [12]. Furthermore, the method included m/z 88 and m/z 101 for the determination of the ethyl esters used as internal standards [13,14]. Identification and quantification of the IS d 70 -DSPC was based on m/z 77 and m/z 91 [10,12].…”

Section: Gas Chromatography In Combination With Mass Spectrometry (Gcmentioning

confidence: 99%

Fatty acids and polar lipid content of cheese and mould‐contaminated cheese

Schlosser¹,

Vetter²

2011

Euro J Lipid Sci & Tech

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Unintended infestation of food with viable mould irreversibly leads to spoilage of food. However, little data existed on the fatty acid composition and polar lipid content of mould grown on cheese and the potential influence of these parameters in mould-infested cheese. In this study, we thus focused on the fatty acids composition and polar lipid content of cheese, mould isolated from mould-infested cheese, as well as inoculation of cheese with cultures of isolated moulds. For this purpose, we stored three cheese samples (gouda, brown cheese, and parmesan) over extended periods in order to force mould infestation from air. The moulds grown on gouda and brown cheese were identified as Penicillium brevicompactum and Penicillium roqueforti, respectively, while a mixed culture was obtained from parmesan. From these contaminated samples, mould spores were isolated and cultivated. Finally, the (pure) cheeses were inoculated with mould spores. Three subsamples, (1) cheeses, (2) moulds isolated from cheese, and (3) cheeses infested with the isolated moulds were studied with regards to their fatty acid distribution in the neutral and the polar lipids. The different amounts of polar lipids of cheese (0.39-2%), moulds (11-30.7%), and mould-infested cheese (1.5-5%) allowed calculating the mould content of the mouldinfested cheeses.

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“…[192] 10,11-Dichloroundecanoic acid, synthesized by electrophilic addition of chlorine to UA, was used as an internal standard for recovery checks in quantification of minor fatty acids in food stuff by GC. [193] 7 Surface Chemistry…”

Section: Analytical Aspectsmentioning

confidence: 99%

Undecylenic Acid: A Valuable and Physiologically Active Renewable Building Block from Castor Oil

2009

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A lot of attention is currently being paid to the transition to a biobased economy. In this movement, most efforts concentrate on the development of bioenergy applications including bioethanol, biodiesel, thermochemical conversion of biomass, and others. However, in the energy sector other nonbiomass alternatives are known, whereas no valuable alternatives are available when thinking about chemical building blocks. Therefore, it is also essential to develop new routes for the synthesis of bio-based chemicals and materials derived thereof. Such intermediates can originate either from plants or from animals. Castor oil is a non-edible oil extracted from the seeds of the castor bean plant Ricinus communis (Euphorbiaceae), which grows in tropical and subtropical areas. Globally, around one million tons of castor seeds are produced every year, the leading producing areas being India, PR China, and Brazil.2 10-Undecenoic acid or undecylenic acid is a fatty acid derived from castor oil that, owing to its bifunctional nature, has many possibilities to develop sustainable applications.

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Exclusive quantification of methyl-branched fatty acids and minor 18:1-isomers in foodstuff by GC/MS in the SIM mode using 10,11-dichloroundecanoic acid and fatty acid ethyl esters as internal standards

Cited by 56 publications

References 31 publications

Overcoming the equivalent-chain-length rule with pH-zone-refining countercurrent chromatography for the preparative separation of fatty acids

Overcoming the equivalent-chain-length rule with pH-zone-refining countercurrent chromatography for the preparative separation of fatty acids

Fatty acids and polar lipid content of cheese and mould‐contaminated cheese

Undecylenic Acid: A Valuable and Physiologically Active Renewable Building Block from Castor Oil

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