Excreted/Secreted Proteins from Trypanosome Procyclic Strains

Nten, Celestine Michelle Atyame; Sommerer, Nicolas; Rofidal, Valérie; Hirtz, Christophe; Rossignol, Michel; Cuny, G; Peltier, Jean‐Benoǐt; Geiger, Anne

doi:10.1155/2010/212817

Cited by 49 publications

(40 citation statements)

References 53 publications

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“…using proteomic tool has uncovered a range of proteins which include unfolding and degradation classes of proteins, such as serine, cysteine proteases, and metallopeptidases. These proteases play a part in the physiological and pathological functions that favour invasion of the parasite, its growth in hostile host conditions, evasion of the host immune defence, and hydrolysis of host proteins [19]. …”

Section: Introductionmentioning

confidence: 99%

Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF

et al. 2016

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BackgroundExcretory-secretory (ES) proteins of E. histolytica are thought to play important roles in the host invasion, metabolism, and defence. Elucidation of the types and functions of E. histolytica ES proteins can further our understanding of the disease pathogenesis. Thus, the aim of this study is to use proteomics approach to better understand the complex ES proteins of the protozoa.Methods E. histolytica ES proteins were prepared by culturing the trophozoites in protein-free medium. The ES proteins were identified using two mass spectrometry tools, namely, LC–ESI–MS/MS and LC–MALDI–TOF/TOF. The identified proteins were then classified according to their biological processes, molecular functions, and cellular components using the Panther classification system (PantherDB).ResultsA complementary list of 219 proteins was identified; this comprised 201 proteins detected by LC–ESI–MS/MS and 107 proteins by LC–MALDI–TOF/TOF. Of the 219 proteins, 89 were identified by both mass-spectrometry systems, while 112 and 18 proteins were detected exclusively by LC–ESI–MS/MS and LC–MALDI–TOF/TOF respectively. Biological protein functional analysis using PantherDB showed that 27% of the proteins were involved in metabolic processes. Using molecular functional and cellular component analyses, 35% of the proteins were found to be involved in catalytic activity, and 21% were associated with the cell parts.ConclusionThis study showed that complementary use of LC–ESI–MS/MS and LC–MALDI–TOF/TOF has improved the identification of ES proteins. The results have increased our understanding of the types of proteins excreted/secreted by the amoeba and provided further evidence of the involvement of ES proteins in intestinal colonisation and evasion of the host immune system, as well as in encystation and excystation of the parasite.Electronic supplementary materialThe online version of this article (doi:10.1186/s12014-016-9135-8) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF

et al. 2016

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“…Trypanosome secretome was also investigated by Atyama Nten, et al. and proteins secreted by T. b. gambiense and T. b. brucei were compared . A relatively high number (up to 40%) of secreted proteins specific to one or the other subspecies was detected, suggesting that different mechanisms of interaction with the host might occur.…”

Section: Omics and Hat Biomarkersmentioning

confidence: 99%

Sleeping Sickness in the ‘Omics Era

Tiberti

Sanchez

2018

Proteomics Clinical Apps

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Sleeping sickness is a neglected tropical disease caused by Trypanosoma brucei parasites, affecting the poorest communities in sub-Saharan Africa. The great efforts done by the scientific community, local governments, and non-governmental organizations (NGOs) via active patients' screening, vector control, and introduction of improved treatment regimens have significantly contributed to the reduction of human African trypanosomiasis (HAT) incidence during the last 15 years. Consequently, the WHO has announced the objective of HAT elimination as a public health problem by 2020. Studies at both parasite and host levels have improved our understanding of the parasite biology and the mechanisms of parasite interaction with its mammalian host. In this review, the impact that 'omics studies have had on sleeping sickness by revealing novel properties of parasite's subcellular organelles are summarized, by highlighting changes induced in the host during the infection and by proposing potential disease markers and therapeutic targets.

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“…Trypanosomes were shown to release peptidases both in vitro and in vivo . To improve our knowledge, we attempted to challenge recent transcriptomics and proteomics data from T. brucei . Among the secreted proteins identified, 134 were specific of the parasite bloodstream form, among which 10% were proteases .…”

Section: Proteases Of Trypanosome Secretome: Possible New Therapeuticmentioning

confidence: 99%

“…Two trypanosome peptidases, oligopeptidase B and prolyl oligopeptidase , were insensitive to host inhibitors and will therefore keep their catalytic activity in the infected hosts. Both have been identified in T. brucei and in Trypanosoma cruzi (Chagas disease) . The inability to inhibit these two peptidases is of importance since, in the bloodstream, they will be in contact with host peptide hormones.…”

Section: Proteases Of Trypanosome Secretome: Possible New Therapeuticmentioning

confidence: 99%

Secreted proteases of Trypanosoma brucei gambiense: Possible targets for sleeping sickness control?

2013

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Human African trypanosomiasis (HAT) is caused by trypanosomes of the species Trypanosoma brucei and belongs to the neglected tropical diseases. Presently, WHO has listed 36 countries as being endemic for sleeping sickness. No vaccine is available, and disease treatment is difficult and has life-threatening side effects. Therefore, there is a crucial need to search for new therapeutic targets against the parasite. Trypanosome excreted-secreted proteins could be promising targets, as the total secretome was shown to inhibit, in vitro, host dendritic cell maturation and their ability to induce lymphocytic allogenic responses. The secretome was found surprisingly rich in various proteins and unexpectedly rich in diverse peptidases, covering more than ten peptidase families or subfamilies. Given their abundance, one may speculate that they would play a genuine role not only in classical "housekeeping" tasks but also in pathogenesis. The paper reviews the deleterious role of proteases from trypanosomes, owing to their capacity to degrade host circulating or structural proteins, as well as proteic hormones, causing severe damage and preventing host immune response. In addition, proteases account for a number of drug targets, such drugs being used to treat severe diseases such AIDS. This review underlines the importance of secreted proteins and especially of secreted proteases as potential targets in HAT-fighting strategies. It points out the need to conduct further investigations on the specific role of each of these various proteases in order to identify those playing a central role in sleeping sickness and would be suitable for drug targeting.

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Excreted/Secreted Proteins from Trypanosome Procyclic Strains

Cited by 49 publications

References 53 publications

Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF

Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF

Sleeping Sickness in the ‘Omics Era

Secreted proteases of Trypanosoma brucei gambiense: Possible targets for sleeping sickness control?

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