Excretion of 5'-Nucleotides from Bacterial Cells

Abstract

Incubating the dried cells of Brevibacterium sojae No.425-40 in alkaline buffer, the ex-Then the determination of the optimum condition of the excretion and the investigation on the enzyme responsible for the degradation of endogenous RNA were carried out. In the experiments using sonicate and disrupted cells, it appeared that orthophosphate and Mg++ might be accelerative or essential for the degradation of endogenous RNA phates were not detected. Although it was not clear whether phosphodiesterase concerned with the degradation of intracellular RNA or not, it was suggested that polynucleotide phosphorylase acted mainly on the degradation. to 2% of dried cells in 0.05M carbonate-bicarbonate buffer (pH 10) and incubating it at