Excretion of glutamate from Corynebacterium glutamicum triggered by amine surfactants

Duperray, Florence; Jezequel, D.; Ghazi, Alexandre; Letellìer, Lucienne; Shechter, Emanuel

doi:10.1016/0005-2736(92)90094-3

Cited by 52 publications

(24 citation statements)

References 19 publications

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“…At pH 6.50, the Ô\j1 was 158 mV, a value close to the Ô\j1 determined for other Grampositive bacteria (162 mV for S aureus and 113 mV for S Jaetis (Kashket, 1981) and 190 mV for Corynebacterium glutamicum (Duperray et al, 1992)). The cell membrane also became more permeable to H+ after addition of linoleic acid since a decrease of the Ô\j1 was observed (fig 3).…”

Section: Action Of Ffa On the Transmembrane Electrical Potentialsupporting

confidence: 76%

“…Total cellular K+ initially present in the Propionibacterium cells was evaluated at 1650 nmol mg-1 dry weight, a value close to the 1800 nmol mg-1 determined by Duperray et al (1992) for the closely related species of Corynebacterium glutamicum. Figure 3 represents the effect of increasing concentrations of linoleic acid on the initial rate of K+ efflux.…”

Section: Alterations Of the Cell Membrane Integrity: Potassium Effluxmentioning

confidence: 87%

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Effects of free fatty acids on propionic acid bacteria

Boyaval

Corre

Dupuis

et al. 1995

Lait

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Summary -The seasonal variations in milk fat composition, especially du ring the grazing period, often lead to poor eye formation in Swiss-type cheese. The influence of free fatty acids on the grow1h and metabolism of the dairy propionibacteria has been studied in this work. Linoleic (C 1B : 2 ), laurie (C 12 : 0 ), myristic (C14:0) and oleic acids (C1B:1) inhibited the growth and acid production of P treudenreichii subsp shermanii in the reference medium. The antibacterial activity of Iinoleic acid can be overcome by additions of cholesterol and soya lecithin to the medium. The four species and !WO subspecies of dairy Propionibacterium could be divided into !WO groups according to their high susceptibility to unsaturated fatty acids (P freudenreichii subsp shermanii and subsp freudenreichit) or low susceptibility (P acidipropionici, P jensenii and P thoeniJ). Nevertheless, this inhibitory action of free fatty acids was not observed in milk, retentate media or lactic curd. A total extraction of the milk lipids led to the recovery of this inhibitory effect on Propionibacterium. The possible modes of action of these molecules are discussed on the basis of the observed potassium effluxes and disturbances in the cell membranes.

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Section: Action Of Ffa On the Transmembrane Electrical Potentialsupporting

confidence: 76%

Section: Alterations Of the Cell Membrane Integrity: Potassium Effluxmentioning

confidence: 87%

Effects of free fatty acids on propionic acid bacteria

Boyaval

Corre

Dupuis

et al. 1995

Lait

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“…If membrane integrity were compromised, then the vesicles would be expected to become permeable to H+ and lose their ability to maintain the stable ApH needed to drive acetate and Ala accumulation. Duperray et al (1992) found that glutamate and K+ efflux from cells of a bacterial strain of Corynebacterium glutamicum resulted from treatment with cationic aliphatic amine surfactants. These treatments also resulted in a 34% reduction in the proton-motive force, which is similar to the reduction of ApH-dependent Ala transport that we observed in the presente of 0.01% MON 0818 (Table IV).…”

Section: Discussionmentioning

confidence: 99%

Surfactant-Increased Glyphosate Uptake into Plasma Membrane Vesicles Isolated from Common Lambsquarters Leaves

Riechers¹,

Wax²,

Liebl³

et al. 1994

Plant Physiol.

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“…The tube was cut within the upper layer of oil, and the denatured cells were resuspended in 45 p1 water together with 10 pi of silicone oil with a density of 1.07 g cm-3 to absorb membrane material. After breaking up the cells by sonication, neutralizing the extract by addition of 25 pl buffer (5 M-KOH, 1 Mtriethanolamine) and removing precipitated protein and cell wall material by centrifugation, the extract was used for amino acid analysis as described above (see also Ebbighausen et al, 1989).…”

Section: Methodsmentioning

confidence: 99%

Lysine secretion by wild-type Corynebacterium glutamicum triggered by dipeptide uptake

Erdmann

Weil

Krämer

1993

Journal of General Microbiology

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In Cuvynebactevium glutamicum peptide uptake increases the internal concentration of amino acids and thus triggers amino acid secretion. The peptide uptake system is stimulated by a factor of two in cells grown on pure peptone medium in comparison to peptone media with additional carbon sources. Uptake depends on the protonmotive force and shows a broad substrate spectrum. Peptide uptake is characterized by a K,,, of about 230 PM and a Ymax of 12 nmol min-' (mg dry wt)-' for the peptide lysyl-alanine (Lys-Ala). Lysine secretion in the wild-type of C. glutarnicum does not show Michaelis-Menten-type kinetics as reported for the producing strains DG 52-5 and MH 20-22B. The secretion of lysine depends on the composition of the medium in which the cells were grown prior to the initiation of secretion by peptide uptake. The lack of secretion activity when the cells are shifted to peptone medium in the presence of chloramphenicol indicates that protein synthesis is necessary for this regulatory process.

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Excretion of glutamate from Corynebacterium glutamicum triggered by amine surfactants

Cited by 52 publications

References 19 publications

Effects of free fatty acids on propionic acid bacteria

Effects of free fatty acids on propionic acid bacteria

Surfactant-Increased Glyphosate Uptake into Plasma Membrane Vesicles Isolated from Common Lambsquarters Leaves

Lysine secretion by wild-type Corynebacterium glutamicum triggered by dipeptide uptake

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