1979
DOI: 10.1007/bf00498020
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Excretion of metabolites by hydrogen bacteria

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Cited by 29 publications
(7 citation statements)
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“…3HB would be the reaction product and could be excreted. Excretion of considerable amounts of 3HB by R. eutropha was described many years ago (62). Alternatively, PHB could be thiolytically cleaved and dehydrated to crotonyl-CoA, as was shown for PhaZa1 (61), and subsequently reduced to butyryl-CoA via FabI2 or via an acylCoA dehydrogenase, many of which are present in the R. eutropha genome.…”
Section: Discussionmentioning
confidence: 94%
See 1 more Smart Citation
“…3HB would be the reaction product and could be excreted. Excretion of considerable amounts of 3HB by R. eutropha was described many years ago (62). Alternatively, PHB could be thiolytically cleaved and dehydrated to crotonyl-CoA, as was shown for PhaZa1 (61), and subsequently reduced to butyryl-CoA via FabI2 or via an acylCoA dehydrogenase, many of which are present in the R. eutropha genome.…”
Section: Discussionmentioning
confidence: 94%
“…As a consequence of oxygen limitation, fermentation products, such as ethanol, 2,3-butanediol, and lactate, are formed. It has also long been known that R. eutropha can produce and excrete acids, in particular, 3-hydroxybutyrate and butyrate (62,65), and this finding could have its explanation in the expression of the B1632-to-B1629 operon. The other two new PHB granule-specific proteins (A2001 and A0225) were present in comparably low abundance (less than the phasins PhaP1 to Pha5, the PHB synthase PhaC2, and PHB depolymerases) but had a higher abundance than PhaP6, PhaP7, and the second PHB synthase, PhaC2.…”
Section: Discussionmentioning
confidence: 99%
“…Oxygen limitation was also mentioned in a fermentation of C. necator producing PHB from hydrogen and carbon dioxide instead of glucose Tanaka, 1990, 1991;Vollbrecht et al, 1979, and as high as 62 g/L of P(3HB) was produced in a specially designed fermentor (Ishizaki et al, 1992). While the oxygen limitation mentioned in these studies was based on the relative condition compared with their previous works.…”
Section: Introductionmentioning
confidence: 99%
“…Elimination of the enzymes responsible for the production of PHA in R. eutropha resulted in a strain capable of secreting a large amount of pyruvate and stored more NADH reducing equivalent compared to the wild type (Lu et al 2012;Raberg et al 2014). Additionally, other reduced metabolites such as 2,3-butanediol, ethanol, lactate, butanol, and 3-hydroxybutyrate are also secreted Vollbrecht and Schlegel 1979). There is a growing interest in repurposing these secreted pyruvate and extra energy compounds in these PHA-negative strains into valueadded chemicals, such as biofuels, pharmaceutical precursors, and flavoring agents .…”
Section: Introductionmentioning
confidence: 99%