Excretion of para‐aminohippurate by the kidney of the cat

Eggleton, M. Grace; Habib, Y. A.

doi:10.1113/jphysiol.1949.sp004453

Cited by 21 publications

(9 citation statements)

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“…There is no chemical resemblance between M. GRACE EGGLETON AND Y. A. HABIB the two substances, so that any suggestion of its action as an enzyme inhibitor in depressing secretion cannot be entertained, as was possible in the case of creatinine and p-aminohippuric acid (Eggleton & Habib, 1949b). The phenomenon is, in fact, inexplicable at present.…”

Section: Discussionmentioning

confidence: 84%

“…The general experimental technique has been already described (Eggleton & Habib, 1949a). A few experiments were performed under chloralose and urethane anaesthesia, but nembutal was used in the majority and in the few experiments performed on dogs.…”

Section: Methodsmentioning

confidence: 99%

“…creatinine and ferrocyanide in the dog, but not in man, and creatinine has been used for measurement of G.F.R. in the cat (Eggleton & Habib, 1949a), since its clearance was found to be independent of changing creatinine concentration in the plasma. This clearance has now been compared with that of inulin over as wide a range of concentrations of the two substances as possible and with the concentrations both increasing and decreasing.…”

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confidence: 99%

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The mode of excretion of creatinine and inulin by the kidney of the cat

Eggleton¹,

Habib²

1951

The Journal of Physiology

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Inulin is used at present for the measurement of glomerular filtration rate (G.F.R.) in most species of laboratory animal, including man, and the evidence in favour of the hypothesis that it is freely filtered at the glomerulug and neither secreted nor reabsorbed by the tubule cells is apparently strong (Smith, 1937). Certain other substances have the same clearance as that of inulin in certain species, e.g. creatinine and ferrocyanide in the dog, but not in man, and creatinine has been used for measurement of G.F.R. in the cat (Eggleton & Habib, 1949a), since its clearance was found to be independent of changing creatinine concentration in the plasma. This clearance has now been compared with that of inulin over as wide a range of concentrations of the two substances as possible and with the concentrations both increasing and decreasing.The two clearances were identical under certain conditions, but under others the creatinine/inulin clearance ratio varied between 0-65 and 1-7. When the two are not identical, the evidence suggests that creatinine clearance is the more probable measure of filtration rate. METHODSThe general experimental technique has been already described (Eggleton & Habib, 1949a). A few experiments were performed under chloralose and urethane anaesthesia, but nembutal was used in the majority and in the few experiments performed on dogs. In the latter, urine was collected from one kidney only, by cannulation of the ureter near its upper end, and blood obtained from a femoral artery. Inulin (Kerfoot) was administered intravenously in concentrations ranging from 1 to 20%, either in a single dose, or by constant infusion from a motor-driven syringe following a small initial dose. Creatinine was injected intravenously when a high concentration was required; when a low steady concentration was needed, it was given by the subcutaneous route.Creatinine was estimated as the alkaline picrate, inulin by Cole's modification (unpublished) of Roe's method (1934). In both, colour comparison was made by use of a photoelectric colorimeter.

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Section: Discussionmentioning

confidence: 84%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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The mode of excretion of creatinine and inulin by the kidney of the cat

Eggleton¹,

Habib²

1951

The Journal of Physiology

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“…Studies in Necturus have shown definite tubular reabsorption of PAH (Kinter, 1959). Infusion of PAH into intact dogs and cats with plasma concentrations of PAH exceeding 5-6 mm led to a clearance of PAH less than inulin clearance in some studies, consistent with net reabsorption (Eggleton & Habib, 1950;Schacter & Freinkel, 1951;Josephson et al 1954). In addition, microinjection of PAH into rat proximal tubules and ureteral retrograde perfusion in dogs showed a small amount of PAH absorption (Sonnenberg, Oelert & Baumann, 1965;Baines et al 1968;Cho & Cafruny, 1970).…”

Section: Introductionmentioning

confidence: 88%

“…Studies in isolated perfused proximal rabbit tubules have shown a strong dependence of net secretion on the peritubular concentration of PAH and maximal transport rates have been defined M. L. MACDOUGALL AND T. B. WIEGMANN for the three anatomically distinct segments of the proximal tubule (Shimomura, Chonko & Grantham, 1981). PAH secretion in intact animals is also seen to become maximal with rising plasma concentrations (Eggleton & Habib, 1950; Josephson, Grieg, Kakossaios & Kallas, 1954;Tanner & Isenberg, 1970). This has been attributed to self-suppression of secretion in the postulated absence of PAH reabsorption (Schacter & Freinkel, 1951; Baines, Gottschalk & Lassiter, 1968).…”

Section: Introductionmentioning

confidence: 99%

Excretion of para‐aminohippurate in the isolated perfused rat kidney: net secretion and net reabsorption.

MacDougall

Wiegmann

1988

The Journal of Physiology

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SUMMARY1. The excretion of para-aminohippurate (PAH) in the isolated perfused rat kidney was examined over a wide range of perfusate PAH concentrations (15 /lM to 6 mM). PAH excretion increased steadily over the range of perfusate concentrations, reaching a maximal excretion rate of 3-28 jsmol/min at a free-PAH concentration of 6 mM.2. Tubular transport of PAH was evaluated from the difference between ultrafiltered PAH and excreted PAH. Net PAH secretion was observed at low perfusate free PAH concentrations. Net PAH transport was zero at a perfusate free PAH concentration of 2'1 mm. Above this level there was progressive net reabsorption.3. Probenecid (2-5 mM) decreased PAH secretion to 18% of the initial value at 129 ,tM-free PAH (P < 0 05). Probenecid had no effect on net reabsorption of PAH at high perfusate levels of the anion.4. Alanine (5 mM) decreased net PAH secretion by 50% at low free PAH concentrations (P < 0 05) and decreased net PAH reabsorption by 50% at a free PAH concentration of 6 mm (P < 0-05). These effects could not be related to effects of PAH, probenecid or alanine on glomerular filtration rate (GFR), vascular resistance or electrolyte excretion.5. The results confirm the existence and integrity of the proximal tubular organic anion secretory system in the isolated kidney. In addition, net PAH reabsorption occurs at high perfusate levels.

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Renal Excretion and Tubular Transport of Organic Anions and Cations

Roch-Ramel

Besseghir

Murer

1992

Comprehensive Physiology

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The sections in this article are: Organic Anions Introduction Renal Excretion and Tubular Transport of Organic Anions Mechanisms Involved in Tubular Transport of Organic Anions Conclusions on the Renal Transport of Organic Anions Organic Cations Introduction Renal Excretion and Tubular Transport of Organic Cations Mechanisms Involved in Tubular Transport of Organic Cations General Conclusions

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Excretion of para‐aminohippurate by the kidney of the cat

Cited by 21 publications

References 5 publications

The mode of excretion of creatinine and inulin by the kidney of the cat

The mode of excretion of creatinine and inulin by the kidney of the cat

Excretion of para‐aminohippurate in the isolated perfused rat kidney: net secretion and net reabsorption.

Renal Excretion and Tubular Transport of Organic Anions and Cations

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