Excretion pattern investigation of urinary normal and modified nucleosides of breast cancer patients by RP-HPLC and factor analysis method

Xu, Guowang; Schmid, Hannes; Lu, Xianbo; Liebich, H.M.; Lu, Ping

doi:10.1002/1099-0801(200011)14:7<459::aid-bmc7>3.0.co;2-k

Cited by 51 publications

(29 citation statements)

References 14 publications

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“…The following seven were the most significant ones: cytidine, N2,N2-dimethylguanine, PCNR, 2-methylguanosine, 1-methylguanosine, pseudouridine and 1-methyladenosine. These nucleosides have already been reported as useful biochemical 'indicators' in malignant diseases (Lakings et al, 1977;Koshida et al, 1985;Holstege et al, 1986;Oerlemans and Lange, 1986;Sander et al, 1986;Rasmuson et al, 1987;Boos et al, 1988;McEntire et al, 1989;Gehrke and Kuo, 1990;Schöch et al, 1990a, b;Kvist et al, 1993;Nakano et al, 1993;Topp et al, 1993;Rasmuson and Björk, 1995;Martinow et al, 1998;Xu et al, 2000;Dudley et al, 2004;Yang et al, 2004;Zheng et al, 2005). Cytidine and uridine were found to be much more increased than reported in other publications.…”

Section: Discussionmentioning

confidence: 99%

“…Before the samples could be analysed by HPLC, a treatment is necessary to remove the proteins. Other authors reported on using phenyl boronate affinity column for nucleoside isolations (Koshida et al, 1985;Holstege et al, 1986;Oerlemans and Lange, 1986;Sander et al, 1986;Rasmuson et al, 1987;McEntire et al, 1989;Gehrke and Kuo, 1990;Schöch et al, 1990a;Kvist et al, 1993;Nakano et al, 1993;Topp et al, 1993;Rasmuson and Björk, 1995;Martinow et al, 1998;Xu et al, 2000;Yang et al, 2004) or using the Baker SPE system with aromatic sulphonic acid (Schöch et al, 1990b). These fractionation methods of nucleosides from urine were found to be too complex and time consuming to allow the analyses of large numbers of urine samples.…”

Section: Discussionmentioning

confidence: 99%

“…Table 1 shows a sensitivity of CEA and CA 15 -3 as 12.5 and 29.2%, so the urinary nucleosides had higher diagnostic sensitivity than serum CEA and CA 15 -3. In another study on women with breast cancer, the modified nucleosides had a higher diagnostic sensitivity than CEA and CA 15 -3 (Rasmuson et al, 1987;Xu et al, 2000;Zheng et al, 2005). This is of particular interest, because breast cancer is the major cancer among women all over the world, and the used markers have low diagnostic sensitivity.…”

Section: Discussionmentioning

confidence: 99%

“…Urinary excretion of methylated nucleosides, which results from increased turnover and degradation of RNA, especially from transfer ribonucleic acid (tRNA), has been shown to be excreted in increased amounts in the urine of patients with different types of tumours and AIDS (Nakano et al, 1993;Xu et al, 2000;Yang et al, 2004;Zheng et al, 2005). Elevated levels of modified nucleosides have been found in urine from patients with leukaemia and lymphoma (Rasmuson and Björk, 1995), cancer of the lung (McEntire et al, 1989), oesophagus (Masuda et al, 1993), breast (Rasmuson et al, 1987;Nakano et al, 1993;Sasco et al, 1996;Zheng et al, 2005), renal cell carcinoma (Koshida et al, 1985), ovarian cancer (Oerlemans and Lange, 1986), liver cancer (Yang et al, 2004), tumours of the bladder (Kvist et al, 1993), colon cancer (Holstege et al, 1986) and Hodgkin's disease (Martinow et al, 1998).…”

mentioning

confidence: 99%

“…However, when a broad spectrum of modified nucleosides has to be determined, the preferred method is high-performance liquid chromatography (HPLC) (Lakings et al, 1977;Koshida et al, 1985;Oerlemans and Lange, 1986;Sander et al, 1986;Rasmuson et al, 1987;McEntire et al, 1989;Gehrke and Kuo, 1990;Schöch et al, 1990a, b;Kvist et al, 1993;Nakano et al, 1993;Topp et al, 1993;Rasmuson and Björk, 1995;Martinow et al, 1998;Xu et al, 2000;Yang et al, 2004).…”

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confidence: 99%

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Modified nucleosides: an accurate tumour marker for clinical diagnosis of cancer, early detection and therapy control

et al. 2006

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Modified nucleosides, regarded as indicators for the whole-body turnover of RNAs, are excreted in abnormal amounts in the urine of patients with malignancies. To test their usefulness as tumour markers and to compare them with the conventional tumour markers, fractionated urine samples were analysed using chromatography. The excretion patterns of nucleosides of 68 cancer patients with malignant and benign tumours and 41 healthy controls have been studied. Significant elevations in the total sum and the concentrations of at least three (or four) of indicator nucleosides cytidine, pseudouridine, 2-pyridone-5-carboxamide-N1-ribofuranoside, N2,N2-dimethylguanine, 1-methylguanosine, 2-methylguanosine and 1-methyladenosine indicate a tumour with a sensitivity of 54% (77%) and a specificity of 86% (98%). Using an artificial neural network analysis, a sensitivity of 97% and a specificity of 85% were achieved in differentiating between tumour and control volunteers. The comparison with carcinoembryonic antigen, cancer antigen 15-3 und tissue polypeptide antigen indicates that urinary nucleosides may be useful tumour markers. This study suggests that the simultaneous determination of modified nucleosides and creatinine in urine samples of patients with cancer leads to an advantage to current methods and is a useful method to detect cancer early and to control the success of therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Modified nucleosides: an accurate tumour marker for clinical diagnosis of cancer, early detection and therapy control

et al. 2006

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Metabolomic approach for determination of urinary nucleosides as potential tumor markers using electromigration techniques

Markuszewski¹,

Struck²,

Waszczuk-Jankowska³

et al. 2010

Electrophoresis

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In the postgenome-sequencing era, several large projects have been running recently. Proteomics and other analysis or structural biology are the most active today. Since the late 1990 s, metabolomics has been gaining importance in systems biology, as it provides real-world end points that complement and help in the interpretation of genomic and proteomic data. Comprehensive information about the level changes of numerous metabolites present in the analyzed samples is essential in metabolomic studies. Therefore, the applied analytical techniques must be suitable for the simultaneous analysis of a diverse range of low-molecular-mass endogenous metabolites such as nucleosides at various concentrations and in different matrices, in particular, in urine and serum. In the view of metabolomic study, this domain is obviously significant to understand specific humans' reactions and it can be perceived as a diagnostic and predictive tool in pathological reactions. Since the term "metabolom" has occurred in common scientific use, there have been many publications about possible ways of analysis of nucleosides as metabolites of either oxidative DNA damage or RNA's turnover that are used as the potential tumor markers. Besides, the availability of fast, reproducible and easy to apply analytical techniques that would allow the identification of a large number of metabolites is highly desirable since they may provide detailed information about the progression of a pathological process. This paper, which describes the most relevant electromigration techniques, covers the period starting from the review of Karl H. Schram (Mass Spectrom. Rev. 1998, 17, 131-251) up to the beginning of 2009.

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Urinary profiling investigation of metabolites with cis‐diol structure from cancer patients based on UPLC‐MS and HPLC‐MS as well as multivariate statistical analysis

Zhao

Wang

et al. 2006

J of Separation Science

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Nucleosides are an important class of metabolites and have been investigated as potential tumor biomarkers. A method based on ultra peerformance liquid chromatography (UPLC)-TOF MS was developed to analyze urinary nucleosides and other metabolites with cis-diol structure to distinguish between cancer patients and healthy persons and compare the results with those obtained by HPLC. The data showed that the UPLC method used about one third of the time required by HPLC and achieved a much better chromatographic resolution and increased sensitivity, the number of peaks detected by UV being 79 and 94 for HPLC and UPLC, respectively. With UPLC-TOF MS, more information was obtained about metabolites, the separation of cancer patients from healthy persons was significantly improved, and more potential biomarkers were found. The method based on UPLC-TOF MS is a powerful technique for the study of metabolite profiles.

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Excretion pattern investigation of urinary normal and modified nucleosides of breast cancer patients by RP-HPLC and factor analysis method

Cited by 51 publications

References 14 publications

Modified nucleosides: an accurate tumour marker for clinical diagnosis of cancer, early detection and therapy control

Modified nucleosides: an accurate tumour marker for clinical diagnosis of cancer, early detection and therapy control

Metabolomic approach for determination of urinary nucleosides as potential tumor markers using electromigration techniques

Urinary profiling investigation of metabolites with cis‐diol structure from cancer patients based on UPLC‐MS and HPLC‐MS as well as multivariate statistical analysis

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