Excretory over-expression of Bacillus sp. G1 cyclodextrin glucanotransferase (CGTase) in Escherichia coli: Optimization of the cultivation conditions by response surface methodology

Optimization for the maximum bacteriocin production of Lactobacillus brevis DF01 using response surface methodology

“…If the difference was significant between the mean of the center points and that -, no antimicrobial activity of the factorial points (p<0.05), the optimum would be near or within the experimental design space. Otherwise the optimum would be outside the experimental design space, next experiment that carried out along the path of steepest ascent is necessary (22,23).…”

Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli

“…Response surface methodology (RSM) has some advantages that include less experiment numbers, suitability for multiple factor experiments, search for relativity between factors, and finding of the most suitable condition and forecast response [2]. Recent years, fractional factorial designs (FFD) and RSM, which are statistical techniques for designing experiments, building models, evaluating the effects of factors and searching for the optimum conditions, have successfully been employed for screening significant factors and optimization the medium composition in many bioprocess [1,3,8,9,12,14,16,17].…”

Excretory overexpression of Paenibacillus pabuli US132 cyclodextrin glucanotransferase (CGTase) in Escherichia coli: gene cloning and optimization of the culture conditions using experimental design

“…Indeed, Lo et al (2007) optimized the culture conditions for the production of a recombinant CGTase by RSM, with soluble fusion protein (bacteriocin release protein) as the response data. From their findings, postinduction temperature significantly influences the pro- duction of recombinant CGTase.…”

“…Production of recombinant CGTase is mainly dependent on the cultivation conditions such as postinduction temperature, induction-starting time that was determined by the cell optical density at 600 nm, concentration of isopropyl-β-D-thiogalactopyranoside (IPTG) and post-induction time (Lo et al 2007). Furthermore, the optimization of some physical and environmental parameters is of primary importance due to its impact on the enhancing of production yield.…”

“…Excretory overexpression of CGTase from Bacillus sp. G1 was successfully carried out by co-expression with bacteriocin release protein gene in E. coli (Lo et al 2007). However, during such recombinant CGTase expression, caution must be taken in order to avoid the full-lysis or undesired cell death, which is caused by the overinduction of the recombinant bacteriocin release protein.…”