Exercise affects biological characteristics of mesenchymal stromal cells derived from bone marrow and adipose tissue

Liu, Shengyao; He, Yongbin; Deng, Songyun; Zhu, Wenting; Xu, Shao‐Yong; Ni, Guo‐Xin

doi:10.1007/s00264-017-3441-2

Cited by 18 publications

(15 citation statements)

References 43 publications

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“…5′-GGT TCC GAG TGA AGG AGA CTG-3′ and 5′-TAC CAG GCG CAT CAC AAC TG-3′ for rCRAMP, and 5′-ATC ACC ATC TTC CAG GAG CG-3′ and 5′-CCT TCT CCA TGG TGG TGA AG-3′ for rat glyceraldehyde-3-phosphate dehydrogenase (Gapdh)53,54 . Target mRNA levels were normalized to that of Gapdh.…”

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confidence: 99%

Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats

Yoshitani

Kabata

Arakawa

et al. 2020

Sci Rep

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Implant-related infection is difficult to treat without extended antibiotic courses. However, the long-term use of antibiotics has led to the development of multidrug-and methicillin-resistant Staphylococcus aureus. Thus, alternatives to conventional antibiotic therapy are needed. Recently, mesenchymal stem cells have been shown to have antimicrobial properties. This study aimed to evaluate the antimicrobial activity and therapeutic effect of local treatment with antibiotic-loaded adipose-derived stem cells (ADSCs) plus an antibiotic in a rat implant-associated infection model. Liquid chromatography/tandem mass spectrometry revealed that ADScs cultured in the presence of ciprofloxacin for 24 h showed time-dependent antibiotic loading. Next, we studied the therapeutic effects of ADSCs and ciprofloxacin alone or in combination in an implant-related infection rat model. The therapeutic effects of ADSCs plus antibiotics, antibiotics, and ADSCs were compared with no treatment as a control. Rats treated with ADSCs plus ciprofloxacin had the lowest modified osteomyelitis scores, abscess formation, and bacterial burden on the implant among all groups (P < 0.05). Thus, local treatment with ADSCs plus an antibiotic has an antimicrobial effect in implantrelated infection and decrease abscess formation. Thus, our findings indicate that local administration of ADSCs with antibiotics represents a novel treatment strategy for implant-associated osteomyelitis. Periprosthetic infections are a tremendous burden to patients and healthcare institutions worldwide 1. With the increase in arthroplasty procedures and the ongoing development of drug-resistant microorganisms, the incidence of such infections has been increasing 1. To address this challenge, novel treatments are necessary 1. Staphylococcus aureus (S. aureus) is one of the primary pathogens responsible for implant-associated osteomyelitis 2. The ability of S. aureus to establish chronic, implant-associated infections and our inability to cure them are directly associated with its capacity to form biofilms, creating an environment where the bacteria can grow and persist while being protected from the patient's immune response and antibiotics 3. At present, systemic administration of antibiotics is the standard therapy for implant-associated infections. However, the long-term use of antibiotics has led to the development of multidrug-resistant and methicillin-resistant S. aureus 4. Strategies

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mentioning

confidence: 99%

Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats

Yoshitani

Kabata

Arakawa

et al. 2020

Sci Rep

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“…As a result, comparison and interpretation of the various scientific researches are restricted. Standardization of these parameters may increase reliability and repeatability of results, but there are also factors affecting the quality of ASC, which cannot be standardized, i.e., age, ethnicity, medical history, and BMI index [5, 23, 26–29].…”

Section: Discussionmentioning

confidence: 99%

Characterization and Optimization of the Seeding Process of Adipose Stem Cells on the Polycaprolactone Scaffolds

Kurzyk

Ostrowska

Święszkowski

et al. 2019

Stem Cells International

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The purpose of the current study was to evaluate the usefulness of adipose-derived stem cells (ASCs) for bone injury therapy. Lipoaspirates were collected from the abdomen regions of 17 healthy female donors (mean age 49 ± 6 years) using Coleman technique or Body-jet liposuction. In the present study, the primary objective was the in vitro characteristics of human ASCs. The secondary objective was the optimization of the cell seeding process on 3D-printed scaffolds using polycaprolactone (PCL) or polycaprolactone covered with tricalcium phosphate (PCL + 5% TCP). Biological evaluation of human ASC showed high efficiency of isolation obtaining a satisfying amount of homogeneous cell populations. Results suggest that ASCs can be cultured in vitro for a long time without impairing their proliferative capacity. Growth kinetics shows that the highest number of cells can be achieved in passage 5 and after the 16th passage; there is a significant decrease of cell numbers and their proliferative potential. The percentage of colony forming units from the adipose stem cells is 8% ± 0.63% (p<0.05). It was observed that the accumulation of calcium phosphate in the cells in vitro, marked with Alizarin Red S, was increased along with the next passage. Analysis of key parameters critically related to the cell seeding process shows that volume of cell suspension and propagation time greatly improve the efficiency of seeding both in PCL and PCL + 5% TCP scaffolds. The cell seeding efficiency did differ significantly between scaffold materials and cell seeding methods (p<0.001). Increased seeding efficiency was observed when using the saturation of cell suspension into scaffolds with additional incubation. Alkaline phosphatase level production in PCL + 5% TCP scaffold was better than in PCL-only scaffold. The study results can be used for the optimization of the seeding process and quantification methods determining the successful implementation of the preclinical model study in the future tissue engineering strategies.

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“…This study was approved by the Animal Ethics Committee of Nanfang Hospital, Southern Medical University. The BMSCs were isolated from 4-week-old male Sprague-Dawley rats using a method described previously [21]. The morphology of the cells was observed by optic and inverted phase contrast microscope.…”

Section: Methodsmentioning

confidence: 99%

“…Flow cytometry analysis and cell cycle assay were conducted using a method described in our previous study [21]. Briefly, the isolated cells were collected and incubated with antibodies of CD11b, CD45, CD79, and CD90 according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin

Liu

Deng

et al. 2019

Stem Cells International

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Introduction. The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. Material and Methods. The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. Thereafter, BMSCs were subjected to 10% elongation using a Flexcell strain unit, combined with 20 IU/ml EPO. The proliferation of BMSCs was detected by Cell Counting Kit-8, colony formation assay, and cell cycle assay; meanwhile, the mRNA expression levels of Ets-1, C-myc, Ccnd1, and C-fos were detected by reverse transcription and real-time quantitative PCR (qPCR). The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. The role of the extracellular signal-regulated kinases 1/2 (ERK1/2) in the osteogenesis of BMSCs stimulated by mechanical stretch combined with 20 IU/ml EPO was examined by Western blot. Results. Our results showed that effects of EPO on BMSCs included a dose-response relationship, with the 20 IU/ml EPO yielding the largest. Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. The increase in ALP, mineral deposition, and osteoblastic genes induced by the mechanical stretch–EPO combination was inhibited by U0126, an ERK1/2 inhibitor. Conclusion. EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. The underlying mechanism may be related to the activation of the ERK1/2 signaling pathway.

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Exercise affects biological characteristics of mesenchymal stromal cells derived from bone marrow and adipose tissue

Cited by 18 publications

References 43 publications

Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats

Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats

Characterization and Optimization of the Seeding Process of Adipose Stem Cells on the Polycaprolactone Scaffolds

Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin

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