Exercise before or after refeeding prevents refeeding-induced recovery of cell size after fasting with a different pattern of metabolic gene expressions in rat epididymal adipocytes

Effects of exercise training on adipogenesis of stromal‐vascular fraction cells in rat epididymal white adipose tissue

“…Adipocyte number per fat pad and adipocyte size were determined using a Coulter counter equipped with a 400‐μm orifice tube, a stirred sample chamber, and a multichannel particle analyzer (Multisizer‐3, Coulter Counter; Beckman Coulter, Fullerton, CA, USA), according to the modified method (Sakurai et al. 2007).…”

“…Adipocyte number per fat pad and adipocyte size were determined using a Coulter counter equipped with a 400-lm orifice tube, a stirred sample chamber, and a multichannel particle analyzer (Multisizer-3, Coulter Counter; Beckman Coulter, Fullerton, CA, USA), according to the modified method (Sakurai et al 2007). Briefly, a portion (1 g) of the original fat pad was digested according to the method described above (Bjö rntorp et al 1978), and isolated adipocytes were exactly suspended in a total volume of 5 mL of buffer A.…”

“…plasticity is greatly sensitive to various physiological, nutritional and pathological stimuli. For example, fasting and refeeding induce the reduction and enlargement of WAT respectively (Sakurai et al 2007). Moreover, chronic exercise training has been well known to reduce WAT mass.…”

Effect of exercise training on adipocyte-size-dependent expression of leptin and adiponectin

“…Mean adipocyte diameters were measured in aliquots taken from the original, unfractionated sample, and each separate, filtered fraction using a Coulter counter equipped with a 400-µm-orifice tube, a sample-stirring chamber, and a multichannel particle analyzer (Multisizer-3; Coulter Counter, Beckman Coulter, Inc., CA, USA) according to a method described previously (Sakurai et al 2007). Prepared intact adipocytes were diluted in a 10-ml aliquot of electrolyte solution (Isoton II containing 10% glycerol) and stirred to maintain a homogeneous suspension at 37 ºC.…”

Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation

“…The clinical relevance of lipid handling by the liver has been enlightened by the finding that, when rats are starved and subsequently refed, liver lipids rapidly accumulate, especially when fed carbohydrates [4]. This phenomenon is closely associated to repeated bouts of weight loss and regain, also known as weight cycling or “yo-yo” dieting [5]. At the gene expression level, a food deprivation/refeeding regimen using high-carbohydrate diets results in the upregulation of several hepatic lipogenic enzymes such as fatty acid synthetase (FAS) [6–8] and stearoyl-CoA desaturase1 (SCD1) [9, 10].…”