Background: Obesity is a public health problem worldwide. To widely disseminate weightloss interventions across the target population, a cost-effective approach is needed. Objective: We aimed to test whether a single motivational lecture could promote weight loss. Methods: Our study was a 3-month randomized controlled trial, and we recruited participants via local newspaper advertisements in 3 cities in Ibaraki Prefecture, Japan, and randomly assigned them to a control group (no intervention) and an intervention group, who attended a single motivational lecture lasting approximately 2 h. No other lectures or textbooks were provided. The eligibility criteria included an age of 40-64 years, a body mass index (BMI) of 25-40 kg/ m 2 , and the presence of at least 1 component of metabolic syndrome. The primary outcome was body weight change at 3 months. Results: We enrolled 145 eligible participants with a mean age of 53.8 ± 7.1 years and a BMI of 28.5 ± 3.1 kg/m 2 . The 3-month body weight change in the control and intervention groups was -0.65 kg (95% confidence interval [CI] -1.09 to -0.20) and -2.48 kg (95% CI -3.01 to -1.95), respectively. The between-group difference was 1.83 kg (95% CI 1.15-2.51). Conclusions: The significant difference suggested that a single motivational lecture is an effective option to promote modest weight loss in the short term. motivational lecture was given by an experienced researcher. Standardization of the lecture would be needed to disseminate the information to the public. Finally, we calculated the sample size based on the effect size from our previous RCT [7] and not based on the minimally clinically significant difference between groups. The default difference of 1.5 kg was equivalent to about 2% of baseline weight. Therefore, a single lecture might not be adequate to acquire clinically significant (3%-5%) weight loss.In conclusion, our 3-month RCT tested whether a single motivational lecture could promote weight loss for overweight Japanese adults. Our results suggest that a single lecture can be a cost-effective option to promote modest weight loss in the short term.