Exercise serum regulate uric acid transporters in normal rat kidney cells

Abstract

Objective: In this study, we tested the hypothesis that circulating factors produced during exercise regulate the expression of ABCC4, ABCG2, URAT1, and GLUT9 in normal rat kidney and NRK-52E cells and their relationship with NF-κB and NRF-2. Method: NRK-52E cells were separately cultured by serum from 10 healthy SD rats who did not exercise (CON) and 10 healthy SD rats who did aerobic treadmill exercise for 6 weeks, and cells cultured by serum from rats who did aerobic treadmill exercise for 6 weeks were separated by without NRF-2 inhibitor (EXE) and with NRF-2 inhibitor (EXE+ML). results: Aerobic exercise reduced serum uric acid level in rats, but had no significant effect on liver xanthine oxidase. It also increased the expression of some uric acid transporters in kidney and NRK-52E cells and increased the ability of cells in uric acid excretion. When the NRF-2 were inhibited, the expression of NF-κB and ABCG2 increased, and the expression of ABCC4, URAT1 and GLUT9 decreased. Conclusion: this study suggested that 6 weeks of aerobic treadmill exercise intervention may help to improve the excretion of uric acid in renal cells, suggesting that long-term aerobic exercise may be a means to prevent or treat hyperuricemia.