Exercise training affects age-induced changes in SOD and heat shock protein expression in rat heart

Rinaldi, Barbara; Corbi, Graziamaria; Boccuti, Silvia; Filippelli, Walter; Rengo, Giuseppe; Leosco, Dario; Rossi, Francesco; Filippelli, Amelia; Ferrara, Nicola

doi:10.1016/j.exger.2006.05.008

Cited by 83 publications

(71 citation statements)

References 47 publications

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“…Moreover, the three weeks of aerobic training and DOX administration (20mg.kg-1) significantly increased HSP 70 and SOD, while causing a significant decrease in MDA compared to the control+DOX group (20mg.kg-1). However, the findings demonstrated that the exercise could protect the heart against DOX-induced damage (3,31). These findings are consistent with the findings of our previous study that showed the cardioprotective role of endurance training in DOX-treated rats (22).…”

Section: Discussionsupporting

confidence: 83%

“…Physical activity is able to reduce the generation of oxidants during ischemiareperfusion injury and plays a calciumprotective role via activation of the ROS scavenger enzyme (MnSOD). This improved oxidative status consequent to a correct physical activity program is partially responsible for some benefits such as decreased arterial stiffness, improved endothelial function, metabolic and clotting setting, and reduced body weight (31,34). An interesting finding that may provide further insight into the effects of DOX on the myocardium was a slight decrease in HSP 70 protein content in the heart of DOX-treated rats (36).…”

Section: Discussionmentioning

confidence: 89%

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Age-Related Changes in Doxorubicin-Induced Oxidative Damage: Protective and Pretreatment Effects of Short-Term Aerobic Exercise

Chashmi¹,

Roshan²,

Azizi³

2016

mljgoums

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Background and Objective: Doxorubicin (DOX) is an effective anticancer drug. It has been shown that a short-term exercise performed prior to DOX-treatment has no effect on cardiotoxicity in young rats. In the present study, old and young rats were evaluated to determine the protective effects of pre-treatment with short-term exercise on DOX-induced oxidative damage in cardiac tissue.Methods: Forty-eight male Wistar rats were randomly divided into two groups of young and old, and later divided into three sub-groups of young+DOX, young+training+DOX, young+training+salin, old+DOX, old+training+DOX and old+training+salin. The training protocol included treadmill running for 25-39 min/day at 15-17 m/min, 5 days/week for three weeks. All treatments were carried out 24h after the last exercise bout. The rats were sacrificed 48h after DOX administration.Results: Although DOX injection significantly affected the cardiac tissue of old rats compared to young rats, pretreatment with endurance training in DOX-treated rats caused an increase in Heat shock protein (3.02% vs. 23.36) and superoxide dismutase (30.12% vs. 31.12), and a decrease in malondialdehyde (10.92% vs. 19.60) in both old and young rats.Conclusion: Although DOX-induced production of free radicals and cardiotoxicity in aged rats is more than that in young rats, the short-term aerobic exercise reduced the damaging effects of free radicals in the old rats more than in young ones. The concentration of antioxidant enzymes also increases with exercise in the old rats compared to young rats.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 89%

Age-Related Changes in Doxorubicin-Induced Oxidative Damage: Protective and Pretreatment Effects of Short-Term Aerobic Exercise

Chashmi¹,

Roshan²,

Azizi³

2016

mljgoums

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“…Given the previously reported time period needed for the translation of the HSP70 transcript, our results suggest that regular exercise itself could lead to an elevation in constitutive levels of HSP70 within the myocardium (Melling et al, 2007). Moreover, it has been previously demonstrated that endurance training increases the protein expression of HSP70 in control aged hearts (Rinaldi et al, 2006). Thus, increased HSP70 content in aged trained hearts could participate to an augmented intrinsic resistance to ischemia-reperfusion.…”

Section: Discussionsupporting

confidence: 59%

“…We show here that regular physical exercise strongly decreases protein carbonylation, the most widely studied modification induced by ROS, in senescent hearts after IR. Such a limitation in cellular oxidative damage could be due to an increase in antioxidant status Quindry et al, 2005;Rinaldi et al, 2006) and/or a reduced production of ROS (Judge et al, 2005). These results suggest that an augmented resistance to oxidative stress by training would participate to the improvement in post-ischemic recovery regarding, respectively, contractile function by decreasing protein oxidation and coronary flow by attenuating the premature breakdown of NO by ROS and/or limiting oxidative damage in coronary endothelial and smooth muscle cells (Linke et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Exercise training improves functional post-ischemic recovery in senescent heart

Page

Noirez

Courty

et al. 2009

Experimental Gerontology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. and eNOS myocardial contents. ACCEPTED MANUSCRIPT

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“…The expression of MnSOD, Cu/ZnSOD or CAT by NRK-52E cells incubated with DMEM containing 1 or 30 mM paraquat for 24 and 4 hours respectively was determined by Western blot analysis as described previously (Rinaldi et al, 2006). At the end of the incubation period the cell monolayers were rinsed 3-5 times with ice cold PBS followed by the addition of "RIPA buffer" (150mM NaCl, 50mM Tris-HCl (pH 7.4), 1mM ethylenediaminetetraacetic acid, 1mM phenylmethylsulfonylfluoride, 1% Triton-X100, 1% deoxycholic sodium salt and 0.1% SDS) containing cocktail inhibitor and the cells were kept on ice for 30 minutes.…”

Section: Measurement Of the Expression Of Antioxidant Enzymesmentioning

confidence: 99%

Modulation of Antioxidant Enzyme Expression and Activity by Paraquat in Renal Epithelial NRK-52E Cells

Samai¹,

Boccuti²,

Samai³

et al. 2011

Sierra Leone j. biomed. res.

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Renal toxicity produced by paraquat involves the generation of reactive oxygen species (ROS) which can overwhelm antioxidant defences, leading to oxidant injury. However, there are conflicting reports regarding the activity and/or expression of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) during oxidative stress injury. This study investigated the activity and expression of these enzymes in a renal epithelial cell line following exposure to paraquat. Confluent NRK-52E cells were incubated with increasing concentrations of paraquat (1-100mM) for up to 24 hours. Renal cell death was determined by measurement of lactate dehydrogenase release. Oxidant damage was determined via measurement of malondialdehyde formation and DNA strand breaks. The effects of paraquat on DNA and de novo protein synthesis were determined using radio-labelled thymidine and leucine respectively. ROS generation (superoxide anion and hydroxyl radical formation) was measured using nitrobluetetrazolium and deoxyribose assays. Antioxidant enzyme activities and expression were measured using established biochemical assays and Western blot analysis. Exposure of confluent NRK-52E cells to paraquat resulted in significant cell death involving increased lipid peroxidation, DNA damage and inhibition of DNA and de novo protein synthesis. Renal cell injury and death were secondary to increased ROS generation. Incubation with paraquat reduced SOD and CAT activities; in contrast, GSH-Px activity increased significantly. Although SOD expression was significantly reduced, catalase expression was unaffected. These results indicate that paraquat mediates renal toxicity via oxidative stress involving both an increase in ROS generation and reductions in SOD and CAT activities with a concomitant reduction in SOD expression.

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Exercise training affects age-induced changes in SOD and heat shock protein expression in rat heart

Cited by 83 publications

References 47 publications

Age-Related Changes in Doxorubicin-Induced Oxidative Damage: Protective and Pretreatment Effects of Short-Term Aerobic Exercise

Age-Related Changes in Doxorubicin-Induced Oxidative Damage: Protective and Pretreatment Effects of Short-Term Aerobic Exercise

Exercise training improves functional post-ischemic recovery in senescent heart

Modulation of Antioxidant Enzyme Expression and Activity by Paraquat in Renal Epithelial NRK-52E Cells

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