Exercise training has beneficial anti-atrophy effects by inhibiting oxidative stress-induced MuRF1 upregulation in rats with diabetes

Mou, Cai-Ying; Yang, Yue; Wang, Song; Zhao, Zhiwei

doi:10.1016/j.lfs.2011.04.018

Cited by 40 publications

(32 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One of the major targets of PI-3K, the serine threonine kinase Akt, was found to play a central role in β-cell growth and survival (21,22). An in vivo study revealed that overexpression of constitutively active Akt in mice pancreatic β cells resulted in marked expansion of β-cell mass by increasing the β-cell proliferation and size (23).…”

Section: Discussionmentioning

confidence: 99%

The Akt/FoxO1/p27 pathway mediates the proliferative action of liraglutide in β cells

et al. 2011

View full text Add to dashboard Cite

Abstract. Numerous studies have shown that liraglutide, a modified form of human glucagon-like peptide-1 (GLP-1), increases β-cell mass. However, the underlying molecular mechanisms remain unclear. In the present study, we investigated the role of Akt/FoxO1/p27 signaling in liraglutide-induced β-cell proliferation. INS-1 rat insulinoma cells were exposed to two different concentrations of liraglutide. MTT assay was performed to evaluate β-cell proliferation. The expression of Akt/FoxO1/p27 was detected by quantitative real-time PCR and Western blotting. The results revealed that in comparison to the non-treatment group, stimulating INS-1 cells with 10 and 100 nM liraglutide caused β-cell proliferation to be significantly enhanced. The mRNA levels of p27 in INS-1 cells declined upon treatment with liraglutide compared to the non-treatment group. Western blot analysis revealed that the phosphorylation of Akt and FoxO1 was markedly elevated following exposure to liraglutide. Moreover, LY294002, a phosphatidylinositol 3-kinase (PI-3K) inhibitor, significantly abrogated liraglutide-induced effects. Therefore, we conclude that liraglutide increased the β-cell mass by upregulating β-cell proliferation and that the proliferative action of liraglutide in β cells was mediated by activation of PI-3K/Akt, which resulted in inactivation of FoxO1 and decreased p27.

show abstract

Section: Discussionmentioning

confidence: 99%

The Akt/FoxO1/p27 pathway mediates the proliferative action of liraglutide in β cells

et al. 2011

View full text Add to dashboard Cite

show abstract

“…So we aimed to evaluate the impact of the anaerobic training on the biomarkers of proteolysis in the gastrocnemius mus cle of diabetic rats. For this the expression of different genes Ubb, E2-14kDa, Trim63 (12,29) related to ubi quitinproteasome proteolytic pathway was analyzed. We detected that diabetes caused an elevation in the Trim63 mRNA level (Figure 2C), but the anaerobic training was efficient to reduce this level, suggesting that this gene could be modulated by regular exercise.…”

Section: Discussionmentioning

confidence: 99%

“…The protocol of jump in the liquid medium have been widely used with ex perimental models, however, the literature still needs further studies regarding its influence on the protein degradation. The exercise can modulate the pattern of expression of the proteins involved in the proteolysis process (12). It is known that the conjugating enzyme E214kDa, when expressed above basal levels, is con sidered a biomarker of the activation of UPS system (4).…”

mentioning

confidence: 99%

Six-week anaerobic training improves proteolytic profile of diabetic rats

Oliveira-Batista

Silva

Passos

et al. 2015

Arch. Endocrinol. Metab.

View full text Add to dashboard Cite

Objective: To evaluate the effect of six-week anaerobic training on the mRNA expression of genes related to proteolysis Ubb (Ubiquitin), E2-14kDa, Trim63 (MuRF1 protein) and Nfkb1 in the skeletal muscle of diabetic rats. Materials and methods: Four groups were established: DE (Diabetes Exercised), DS (Diabetes Sedentary), CE (Control Exercised) and CS (Control Sedentary). The training consisted of 3 sets of 12 jumps in the liquid mean with load equivalent to 50% of BW for 6 weeks. Euthanasia occurred under ip anesthesia, and blood, adipose tissue and skeletal muscles were collected. Gene expression was quantified by RT-PCR in the gastrocnemius muscle. ANOVA one-way was used for comparison among groups, with post-hoc (Tukey) when necessary, considering p < 0.05. Results: We observed reduction in the body weight and adipose tissue in the diabetic groups. The muscle mass was reduced in DS, which could be reversed by training (DE). Although DS and DE have presented similar body weight, the training protocol in DE promoted reduction in the adipose tissue, and increase of muscle mass. Anaerobic training was efficient to reduce glycaemia only in the diabetic animals until 6 hours after the end of training. The Trim63 gene expression was increased in DS; decreased Ubb gene level was observed in trained rats (CE and DE) compared to sedentary (CS and DS), and DE presented the lowest level of E2-14kDa gene expression. Conclusion: Six-week anaerobic training promoted muscle mass gain, improved glycemic control, and exerted inhibitory effect on the proteolysis of gastrocnemius muscle of diabetic rats. Arch Endocrinol Metab. 2015;59(5):400-6

show abstract

“…Regular exercise training has shown beneficial anti-atrophy effects [11] in diabetic rats and improved skeletal muscle insulin resistance in obese Zucker rat rats [12]. Animal models were used to study the exercise-mediated effects on the regulation of lipid metabolism using guinea pigs [13].…”

Section: Lessons From Pre-clinical and Clinical Researchmentioning

confidence: 99%

Physical activity and health: Shifting focus from modern era to Stone Age life style

Mohan¹,

Selvarajan²

2017

Phys Med Rehabil Res

View full text Add to dashboard Cite

Exercise training has beneficial anti-atrophy effects by inhibiting oxidative stress-induced MuRF1 upregulation in rats with diabetes

Cited by 40 publications

References 32 publications

The Akt/FoxO1/p27 pathway mediates the proliferative action of liraglutide in β cells

The Akt/FoxO1/p27 pathway mediates the proliferative action of liraglutide in β cells

Six-week anaerobic training improves proteolytic profile of diabetic rats

Physical activity and health: Shifting focus from modern era to Stone Age life style

Contact Info

Product

Resources

About