Exhaled nitric oxide and biomarkers in exhaled breath condensate indicate the presence, severity and control of childhood asthma

Robroeks, Charlotte M. H. H. T.; Kant, Kim D. G. van de; Jöbsis, Quirijn; Hendriks, Hanneke; Gent, René van; Wouters, Emiel F.M.; Damoiseaux, Jan; Bast, Aalt; Wodzig, Will K. W. H.; Dompeling, Edward

doi:10.1111/j.1365-2222.2007.02788.x

Cited by 118 publications

(93 citation statements)

References 38 publications

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“…EBC lyophilisation can concentrate samples up to a factor of 30 times, thus improving sensitivity [ 61 ]. There was no relationship between EBC cytokine concentrations and storage times up to 1 year in samples stored at − 80 ° C [ 157 ]. With newer technologies, EBC cytokine measurement may become useful in assessing airway inflammatory status for treatment monitoring and investigating disease pathophysiology [ 156 ].…”

Section: Cytokines Chemokines and Growth Factorsmentioning

confidence: 99%

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

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Since the late 1990s, a surge in interest in the analysis of exhaled breath condensate (EBC) resulted in the American Thoracic Society and European Respiratory Society (ATS/ERS) organising a Task Force in 2001 to develop guidelines on EBC collection and measurement of biomarkers. This Task Force published their guidelines in 2005 based on literature and expert opinions at that time, and multiple shortcomings and knowledge deficits were also identified. The clinical application of EBC collection and its biomarkers are currently still limited by several of these knowledge gaps, hence further guidelines for standardisation are required to ensure external validity. Using related articles produced since the publication of the ATS/ERS Task Force report, this paper attempts to provide a comprehensive update to the original guideline and review the methodological shortcomings identified. This review can hopefully serve as a yardstick for future studies involving this emerging clinical tool.

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Section: Cytokines Chemokines and Growth Factorsmentioning

confidence: 99%

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

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show abstract

“…FeNO levels did not significantly differ in women before and after they lost asthma control. 84 In a prospective study that followed patients who were mostly on ICS (age 10 and over) for 12 weeks, FeNO did not correlate with ACQ or with shortened ACQ (without FEV 1 ). 86 In terms of the use of FeNO to predict asthma exacerbations, several studies showed higher FeNO values in patients who had had exacerbations prior to the test (retrospective analysis) or had developed exacerbations after the test (prospective analysis).…”

Section: Kq1b Risk Of Bias Assessment Of Observational Studiesmentioning

confidence: 99%

The Clinical Utility of Fractional Exhaled Nitric Oxide (FeNO) in Asthma Management

Wang¹,

Pianosi²,

Keogh³

et al. 2017

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“…The ability to non-invasively characterize airway tissue by repeated measurements of biomarkers in EBC would be invaluable for studying the time-course of dynamic inflammatory pathways that are involved in asthma exacerbation. Ultimately, EBC biomarkers may contribute to the assessment of different asthma phenotypes and the development of individualized rational approaches to asthma management at the point of care [6][7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Toward point-of-care management of chronic respiratory conditions: Electrochemical sensing of nitrite content in exhaled breath condensate using reduced graphene oxide

et al. 2017

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We present a portable non-invasive approach for measuring indicators of inflammation and oxidative stress in the respiratory tract by quantifying a biomarker in exhaled breath condensate (EBC). We discuss the fabrication and characterization of a miniaturized electrochemical sensor for detecting nitrite content in EBC using reduced graphene oxide. The nitrite content in EBC has been demonstrated to be a promising biomarker of inflammation in the respiratory tract, particularly in asthma. We utilized the unique properties of reduced graphene oxide (rGO); specifically, the material is resilient to corrosion while exhibiting rapid electron transfer with electrolytes, thus allowing for highly sensitive electrochemical detection with minimal fouling. Our rGO sensor was housed in an electrochemical cell fabricated from polydimethyl siloxane (PDMS), which was necessary to analyze small EBC sample volumes. The sensor is capable of detecting nitrite at a low over-potential of 0.7 V with respect to an Ag/AgCl reference electrode. We characterized the performance of the sensors using standard nitrite/buffer solutions, nitrite spiked into EBC, and clinical EBC samples. The sensor demonstrated a sensitivity of 0.21 μA μM − 1 cm − 2 in the range of 20-100 μM and of 0.1 μA μM − 1 cm − 2 in the range of 100-1000 μM nitrite concentration and exhibited a low detection limit of 830 nM in the EBC matrix. To benchmark our platform, we tested our sensors using seven pre-characterized clinical EBC samples with concentrations ranging between 0.14 and 6.5 μM. This enzyme-free and label-free method of detecting biomarkers in EBC can pave the way for the development of portable breath analyzers for diagnosing and managing changes in respiratory inflammation and disease.

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Exhaled nitric oxide and biomarkers in exhaled breath condensate indicate the presence, severity and control of childhood asthma

Abstract: Different markers in condensate are of an additional value to exhaled nitric oxide, and are needed in non-invasive inflammometry. They could be useful to diagnose asthma and to indicate asthma control and severity in childhood.

Cited by 118 publications

References 38 publications

Exhaled breath condensate: a comprehensive update

Exhaled breath condensate: a comprehensive update

The Clinical Utility of Fractional Exhaled Nitric Oxide (FeNO) in Asthma Management

Toward point-of-care management of chronic respiratory conditions: Electrochemical sensing of nitrite content in exhaled breath condensate using reduced graphene oxide

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