Exhaled nitric oxide as a marker of airway inflammation for an epidemiologic study in schoolchildren

Saito, Junpei; Inoue, Keiichi; Sugawara, Akiko; Yoshikawa, Motoko; Watanabe, Kana; Ishida, Takashi; Ohtsuka, Yoshinori; Munakata, Mitsuru

doi:10.1016/j.jaci.2004.05.033

Cited by 79 publications

(68 citation statements)

References 31 publications

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“…BUCHVALD et al [7] have also noted significantly higher FeNO levels in non-Caucasian children (Asians, AfricanAmericans and Hispanics). Consistent with the current study, FeNO levels in a relatively small group of Japanese children aged 10-12 yrs who were nonatopic and had never experienced wheeze [17], were also higher than those of Caucasian children [6][7][8].…”

Section: Discussionsupporting

confidence: 89%

“…is an important determinant of FeNO, even in healthy children who have no history of allergic diseases and have no current or past symptoms suggestive of allergic diseases. This finding not only confirms the previously reported association of allergic sensitisation with FeNO [6,17,29,32] but further indicates that the association is quantitative and independent of symptoms. As such, a hypothesis is proposed here that FeNO elevation may denote a constitutional feature of allergic sensitisation.…”

Section: Exhaled Biomarkers T-c Yao Et Alsupporting

confidence: 91%

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Reference values of exhaled nitric oxide in healthy Asian children aged 5 to 18 years

Yao

Wi²,

Ls³

et al. 2011

Eur Respir J

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This study was undertaken to establish reference values of exhaled nitric oxide fraction (FeNO) and its determinants in healthy Asian children.693 healthy Asian children aged 5-18 yrs were assessed using a single-breath online FeNO measurement (exhaled flow 50 mL?s -1 ), questionnaires, anthropometric measurements, spirometry and total and specific immunoglobulin (Ig) E.Geometric mean FeNO and the upper 95% CI were 13.7 ppb and 49.7 ppb, respectively, for healthy children, and 11.2 ppb and 30.2 ppb, respectively, for those without allergic sensitisation. FeNO was positively associated with age, allergic sensitisation, total IgE, ambient nitric oxide, measurement in the afternoon, and drinking water within 1 h before testing, and was negatively associated with weight. In healthy children without allergic sensitisation, age was the single best explanatory variable. The FeNO predicted values were 1-2 ppb higher in Asian than in Caucasian children in earlier studies, while the upper 95% CI were 9-10 ppb higher.In conclusion, the upper limits of normal FeNO in Asian children depend on age, from 21 ppb in young children to 39 ppb in adolescents. Ethnicity, age, allergic sensitisation, total IgE, ambient nitric oxide, time of testing, drinking water and weight are important determinants.

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Section: Discussionsupporting

confidence: 89%

Section: Exhaled Biomarkers T-c Yao Et Alsupporting

confidence: 91%

Reference values of exhaled nitric oxide in healthy Asian children aged 5 to 18 years

Yao

Wi²,

Ls³

et al. 2011

Eur Respir J

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“…Fractional concentration of NO (FeNO) is measurably higher in children with eosinophilic airway inflammation and active asthma or allergic airway diseases, conditions in which airway inflammation plays a prominent role (1)(2)(3). Based on the findings in children, FeNO has been suggested as a biomarker that reflects important aspects of airway inflammation that may be useful in the management of some children with asthma (4).…”

mentioning

confidence: 99%

DNA Methylation in the Arginase–Nitric Oxide Synthase Pathway Is Associated with Exhaled Nitric Oxide in Children with Asthma

Breton¹,

Byun

Wang³

et al. 2011

Am J Respir Crit Care Med

100

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Rationale: Genetic variation in arginase (ARG) and nitric oxide synthase (NOS) has been associated with exhaled nitric oxide (FeNO) levels in children. Little is known about whether epigenetic variation in these genes modulates FeNO. Objectives: To evaluate whether DNA methylation in ARG and NOS genes is associated with FeNO. Methods: A subset of 940 participants in the Children's Health Study were selected for this study. Children were eligible if they had FeNO measurements and buccal cells collected on the same day. CpG loci located in the promoter regions of NOS1, NOS2A, NOS3, ARG1, and ARG2 genes were analyzed. Multiple loci in each gene were evaluated individually and averaged together. DNA methylation was measured using a bisulfite-polymerase chain reaction pyrosequencing assay. Linear regression models were used to investigate the association between DNA methylation and FeNO and whether associations differed by asthma status. Measurements and Main Results: DNA methylation in ARG2 was significantly associated with FeNO. A 1% increase in average DNA methylation of ARG2 was associated with a 2.3% decrease in FeNO (95% confidence interval, 24 to 20.6). This association was significantly larger in children with asthma (%diff ¼ 28.7%) than in children with no asthma (%diff ¼ 21.6%; p int ¼ 0.01). Differences in FeNO by asthma status were also observed for ARG1 (%diff asthma ¼ 24.4%; %diff non-asthma ¼ 0.3%; p int ¼ 0.02). DNA methylation in NOS genes was not associated with FeNO. Conclusions: DNA methylation in ARG1 and ARG2 is associated with FeNO in children with asthma and suggests a possible role for epigenetic regulation of nitric oxide production.Keywords: arginase; nitric oxide synthase; FeNO; asthma Nitric oxide (NO) is involved in the pathophysiology of allergic airway diseases. Fractional concentration of NO (FeNO) is measurably higher in children with eosinophilic airway inflammation and active asthma or allergic airway diseases, conditions in which airway inflammation plays a prominent role (1-3). Based on the findings in children, FeNO has been suggested as a biomarker that reflects important aspects of airway inflammation that may be useful in the management of some children with asthma (4).NO is synthesized from L-arginine by three NO synthase (NOS) isoforms: (1) neuronal NOS (nNOS encoded by NOS1); (2) endothelial NOS (eNOS encoded by NOS3); and (3) inducible NOS (iNOS encoded by NOS2A). The availability of intracellular L-arginine is a rate-limiting factor in NO production (5). Arginase (ARG) competes with NOS for the common substrate L-arginine. In humans, two isoforms of ARG (encoded by ARG1 and ARG2) are recognized. All three NOS isoforms are expressed in airway epithelium (6-8). Among ARG isoforms, ARGI is located in the cytosol, whereas ARGII is located in the mitochondrial matrix (8). Both isoforms are expressed in the airway epithelium, smooth muscle, and peribronchial and perivascular connective tissues (9, 10). Therefore, differences in ARG and NOS expression levels or function caused ...

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“…(1)(2)(3) NO concentration in human breath is associated with airway inflammatory disorders such as asthma and bronchiectasis. (4)(5)(6) Although monitoring NO concentration in human breath is one of the best noninvasive screening tests for the early diagnosis of these inflammatory disorders, this screening process requires an analytic device with sufficient accuracy at the ppb level.…”

Section: Introductionmentioning

confidence: 99%

Effect of Noble Metal Addition on Co3O4-Based Gas Sensors for Selective NO Detection

2016

Sensors and Materials

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Keywords: oxide, semiconductor, noble metal, transmission electron microscopy, gas sensor P-type semiconductor-based gas sensors have been fabricated for the selective detection of 50-200 ppb nitric oxide (NO). To fabricate the sensors, cobalt oxide (Co 3 O 4 ) powders containing a noble metal (Pd, Ag, Pt, or Au) at 10 wt% were prepared by a colloidal mixing method and deposited on interdigitated Pt electrodes. The sensing responses to 50 and 200 parts per billion (ppb) NO and 25 and 100 parts per million (ppm) H 2 in air were investigated. The sensors with 10 wt% Pd, Ag, Pt, or Au loaded on the Co 3 O 4 powder demonstrated a greater response to NO than Co 3 O 4 with no noble metal content. The addition of Ag was found to suppress the H 2 response, a phenomenon that is attributed to the surface modification effects of Ag on the Co 3 O 4 surface. Furthermore, the sensor with 5 wt% Pd and 5 wt% Ag loaded on the Co 3 O 4 showed a high response in the presence of 50 ppb NO, as well as good selectivity against H 2 .

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Exhaled nitric oxide as a marker of airway inflammation for an epidemiologic study in schoolchildren

Cited by 79 publications

References 31 publications

Reference values of exhaled nitric oxide in healthy Asian children aged 5 to 18 years

Reference values of exhaled nitric oxide in healthy Asian children aged 5 to 18 years

DNA Methylation in the Arginase–Nitric Oxide Synthase Pathway Is Associated with Exhaled Nitric Oxide in Children with Asthma

Effect of Noble Metal Addition on Co3O4-Based Gas Sensors for Selective NO Detection

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