Minimizing the environmental impacts is a challenging task to achieve sustainability in road constructions. Although they are only temporary, the environmental burdens of building activities can have a great impact on the environment and communities, and must be properly assessed and mitigated. A comprehensive evaluation of the impacts requires the consideration of all construction activities, construction sites and the type and operation time of off-road machines and plants that will be used in each site. In this paper, a case study relating to the project of a motorway was carried out with the following objectives: (i) to estimate the dust and gases arising from the whole construction process and identify the most critical pollutants in terms of emitted quantity; (ii) to investigate the worksites, activities and processes with the greatest impact from an emissive standpoint, and (iii) to propose a rational approach for designing and putting in place effective mitigation measures. Carbon oxide (CO), nitrogen oxides (NOx), and fine particulate matter (PM10) emissions have been estimated by applying different models, methodologies and databases, depending on the construction process under analysis, and an emissive balance sheet has been produced. Results showed that CO is the pollutant released in the greatest quantity, followed by NOx. The emission of PM10, mainly due to the movement of trucks on unpaved roads, is one order of magnitude less with respect to CO and NOx, but produces the most perceived and undesired effects of the construction process in the interested communities. Tunnels and bridge are the components of a road with the greatest impact in terms of air emissions.