Exhaustive swimming differentially inhibits P2X1 receptor- and α1-adrenoceptor-mediated vasoconstriction in isolated rat arteries

Abstract

Aim: To investigate the effects of exhaustive swimming exercise on P2X1 receptor-and α1-adrenoceptor-mediated vasoconstriction of different types of arteries in rats. Methods: Male Wistar rats were divided into 2 groups: the sedentary control group (SCG) and the exhaustive swimming exercise group (ESEG). The rats in the ESEG were subjected to a swim to exhaustion once a day for 2 weeks. Internal carotid, caudal, pulmonary, mesenteric arteries and aorta were dissected out. Isometric vasoconstrictive responses of the arteries to α,β-methylene ATP (α,β-MeATP) or noradrenaline (NA) were recorded using a polygraph. Results: The exhaustive swimming exercise did not produce significant change in the EC 50 values of α,β-MeATP or NA in vasoconstrictive response of most of the arteries studied. The exhaustive swimming exercise inhibited the vasoconstrictive responses to P2X1 receptor activation in the internal carotid artery, whereas it reduced the maximal vasoconstrictive responses to α1-adrenoceptor stimulation in the caudal, pulmonary, mesenteric arteries and aorta. The rank order of the reduction of the maximal vasoconstriction was as follows: mesenteric, pulmonary, caudal, aorta. Conclusion: Exhaustive swimming exercise differentially affects the P2X1 receptor-and α1-adrenoceptor-regulated vasoconstriction in internal carotid artery and peripheral arteries. The ability to preserve purinergic vasoconstriction in the peripheral arteries would be useful to help in maintenance of the basal vascular tone during exhaustive swimming exercise.

(−)Doxazosin is a necessary component for the hypotensive effect of (±)doxazosin during long-term administration in conscious rats

“…Another similarly inserted tungsten wire was connected to an isometric tension transducer coupled to a polygraph (ERT-884, Youlin Electron Co, Kaifeng, China) [19] to record the change in tension of the preparation. A preload of 0.75 g was applied to the arterial ring preparations, and the preparations were allowed to equilibrate for 1 h in Krebs-Henseleit (K-H) solution with the following composition (mmol/L) [19,20] : NaCl 133, KCl 4.7, NaH 2 PO 4 1.35, NaHCO 3 16.3, MgSO 4 0.61, glucose 7.8, and CaCl 2 2.52 with a pH 7.4. The K-H solution was maintained at 37 °C and aerated with 95% O 2 and 5% CO 2 .…”