This paper aims to investigate the existence and uniqueness of solutions for a sixth‐order differential equation involving nonlocal and integral boundary conditions. Firstly, we obtain the properties of the relevant Green's functions. The existence result of at least one nontrivial solution is obtained by applying the Krasnoselskii–Zabreiko fixed point theorem. Moreover, we also establish the existence of unique solution to the considered problem via Hölder and Minkowski inequalities and Rus's theorem. Finally, two numerical examples are included to show the applicability of our main results.