2007
DOI: 10.1002/bmc.814
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Existence of low‐molecular‐weight thiols in Caenorhabditis elegans demonstrated by HPLC‐fluorescene detection utilizing 7‐chloro‐N‐[2‐(dimethylamino)ethyl]‐2,1,3‐benzoxadiazole‐4‐sulfonamide

Abstract: A highly sensitive and simple method using HPLC-fluorescence detection with 7-chloro-N-[2-(dimethylamino)ethyl]-2,1,3-benzoxadiazole-4-sulfonamide (DAABD-Cl) as a fluorogenic reagent demonstrated the existence of the low-molecular-weight thiols in the extract of Caenorhabditis elegans (C. elegans). The method includes derivatization of the thiols with DAABD-Cl at 40 degrees C for 10 min in borate buffer (pH 9.0) containing TCEP, CHAPS and EDTA, separation of the derivatives on an ODS column and fluorometric de… Show more

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Cited by 11 publications
(13 citation statements)
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“…Imai et al synthesized similar reagents having the benzofurazan structure, i.e., 4-(N-acetylaminosulfonyl)-7-fluoro-2,1, 3-benzoxadiazole (AcABD-F), 4-(N-trichloroacetylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (TCAcABD-F) [121] and 7-chloro-N-[2-(dimethylamino)ethyl]-2,1,3-benzoxadiazole-4-sulfonamide (DAABD-Cl) [122]. Both the reactivity to the thiol and the fluorescence property of the derivative are almost equivalent to ABD-F. DAABD-Cl was used for the determination of thiols in Caenorhabditis elegans [122].…”
Section: Halogeno-benzofurazansmentioning
confidence: 99%
See 1 more Smart Citation
“…Imai et al synthesized similar reagents having the benzofurazan structure, i.e., 4-(N-acetylaminosulfonyl)-7-fluoro-2,1, 3-benzoxadiazole (AcABD-F), 4-(N-trichloroacetylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (TCAcABD-F) [121] and 7-chloro-N-[2-(dimethylamino)ethyl]-2,1,3-benzoxadiazole-4-sulfonamide (DAABD-Cl) [122]. Both the reactivity to the thiol and the fluorescence property of the derivative are almost equivalent to ABD-F. DAABD-Cl was used for the determination of thiols in Caenorhabditis elegans [122].…”
Section: Halogeno-benzofurazansmentioning
confidence: 99%
“…Both the reactivity to the thiol and the fluorescence property of the derivative are almost equivalent to ABD-F. DAABD-Cl was used for the determination of thiols in Caenorhabditis elegans [122].…”
Section: Halogeno-benzofurazansmentioning
confidence: 99%
“…Previously, we have developed a novel method of proteome analysis, called fluorogenic derivatization liquid chromatography/tandem mass spectrometry (FD-LC-MS/MS), which includes the first fluorogenic derivatization (FD) of protein mixtures, isolation of the target proteins with high-performance liquid chromatography (HPLC) separation, followed by enzymatic digestion and identification of the derivatized proteins by LC-tandem mass spectrometry (MS/MS) with the probability-based protein identification algorithm (Asamoto et al, 2007;Masuda et al, 2004 and. This method has high selectivity, sensitivity and repeatability utilizing DAABD-Cl as a fluorogenic reagent and HPLC as a separation technique.…”
Section: Introductionmentioning
confidence: 99%
“…24,25) 5) In the post-genome era, a sensitive quantitative method for differential profiling analyses of clinical proteomics is required to understand the disease progress. Therefore, as a preliminary step, we adopted an FD-LC-MS/ MS method using DAABD-Cl to clarify disease-related liver proteins in hepatocarcinogenesis in transgenic (Tg) and non-transgenic (NTg) mice at three different developmental stages.…”
Section: Application Of the Methodsmentioning
confidence: 99%