Existence of the Rdl mutant alleles among the Anopheles malaria vector in Indonesia

Asih, Puji B. S.; Syahrani, Lepa; Rozi, Ismail Ekoprayitno; Pratama, Nandha R; Marantina, Sylvia Sance; Arsyad, Dian Sidik; Mangunwardoyo, Wibowo; Hawley, William A.; Laihad, Ferdinand; Shinta, Shinta; Supratman, Supratman; Lobo, Neil F.; Syafruddin, Din

doi:10.1186/preaccept-1876233368639637

Cited by 4 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…; Asih et al . ), and the previous suggestion of recent independent selective sweeps centred on rdl in sympatric populations of An. gambiae and An.…”

Section: Resultssupporting

confidence: 59%

Chromosomal inversions and ecotypic differentiation in Anopheles gambiae: the perspective from whole‐genome sequencing

et al. 2016

View full text Add to dashboard Cite

The molecular mechanisms and genetic architecture that facilitate adaptive radiation of lineages remain elusive. Polymorphic chromosomal inversions, due to their recombination-reducing effect, are proposed instruments of ecotypic differentiation. Here we study an ecologically diversifying lineage of An. gambiae, known as the Bamako chromosomal form based on its unique complement of three chromosomal inversions, to explore the impact of these inversions on ecotypic differentiation. We used pooled and individual genome sequencing of Bamako, typical (non-Bamako) An. gambiae, and the sister species An. coluzzii to investigate evolutionary relationships and genome-wide patterns of nucleotide diversity and differentiation among lineages. Despite extensive shared polymorphism and limited differentiation from the other taxa, Bamako clusters apart from the other taxa, and forms a maximally supported clade in neighbor-joining trees based on whole genome data (including inversions) or solely on collinear regions. Nevertheless, FST outlier analysis reveals that the majority of differentiated regions between Bamako and typical An. gambiae are located inside chromosomal inversions, consistent with their role in the ecological isolation of Bamako. Exceptionally differentiated genomic regions were enriched for genes implicated in nervous system development and signaling. Candidate genes associated with a selective sweep unique to Bamako contain substitutions not observed in sympatric samples of the other taxa, and several insecticide resistance gene alleles shared between Bamako and other taxa segregate at sharply different frequencies in these samples. Bamako represents a useful window into the initial stages of ecological and genomic differentiation from sympatric populations in this important group of malaria vectors.

show abstract

“…; Asih et al . ), and the previous suggestion of recent independent selective sweeps centred on rdl in sympatric populations of An. gambiae and An.…”

Section: Resultssupporting

confidence: 59%

Chromosomal inversions and ecotypic differentiation in Anopheles gambiae: the perspective from whole‐genome sequencing

et al. 2016

View full text Add to dashboard Cite

show abstract

“…For rdl, the 296G mutation was detected in Guinea and Ivory Coast and is the target site for cyclodiene insecticides (e.g., dieldrin), which have been discontinued from use in malaria control programmes. Despite the ban on dieldrin decades ago, molecular analysis studies have shown that despite the A296G substitution declining in some mosquito populations, it has persisted at high frequencies in others [35][36][37][38][39] . Here we show higher allele frequency of A296G mutation in mosquitoes from Ivory Coast when compared with those from Guinea, corroborating a previous report 40 .…”

Section: Discussionmentioning

confidence: 99%

High-throughput barcoding method for the genetic surveillance of insecticide resistance and species identification in Anopheles gambiae complex malaria vectors

Campos

Phelan

Spadar

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Surveillance of malaria vector species and the monitoring of insecticide resistance are essential to inform malaria control strategies and support the reduction of infections and disease. Genetic barcoding of mosquitoes is a useful tool to assist the high-throughput surveillance of insecticide resistance, discriminate between sibling species and to detect the presence of Plasmodium infections. In this study, we combined multiplex PCR, custom designed dual indexing, and Illumina next generation sequencing for high throughput single nucleotide polymorphism (SNP)-profiling of four species from the Anopheles (An.) gambiae complex (An. gambiae sensu stricto, An. coluzzii, An. arabiensis and An. melas). By amplifying and sequencing only 14 genetic fragments (500 bp each), we were able to simultaneously detect Plasmodium infection; insecticide resistance-conferring SNPs in ace1, gste2, vgsc and rdl genes; the partial sequences of nuclear ribosomal internal transcribed spacers (ITS1 and ITS2) and intergenic spacers (IGS), Short INterspersed Elements (SINE), as well as mitochondrial genes (cox1 and nd4) for species identification and genetic diversity. Using this amplicon sequencing approach with the four selected An. gambiae complex species, we identified a total of 15 non-synonymous mutations in the insecticide target genes, including previously described mutations associated with resistance and two new mutations (F1525L in vgsc and D148E in gste2). Overall, we present a reliable and cost-effective high-throughput panel for surveillance of An. gambiae complex mosquitoes in malaria endemic regions.

show abstract

“…Resistance to dieldrin has somehow been maintained in natural mosquito populations (Asih et al 2012, Kwiatkowska et al 2013, Wondji et al 2011, although the selective forces causing its persistence remain a puzzle. One possibility is that cyclodienes continue to be used as insecticides in private agriculture, but another is that the Rdl-296 mutations confer crossresistance to other insecticides.…”

Section: Discussionmentioning

confidence: 99%

A high throughput multi-locus insecticide resistance marker panel for tracking resistance emergence and spread inAnopheles gambiae

Lucas

Rockett

Lynd

et al. 2019

Preprint

View full text Add to dashboard Cite

The spread of resistance to insecticides in the mosquito vectors of diseases such as malaria and dengue poses a threat to the effectiveness of control programmes, which rely largely on insecticide-based interventions. Monitoring the resistance status of mosquito populations is therefore essential, but obtaining direct phenotypic measurements of resistance is laborious and error-prone. In contrast, high-throughput genotyping offers the prospect of quick and repeatable estimates of resistance, while also allowing the genotypic markers of resistance to be tracked and studied. We developed a panel of 28 known or putative markers of resistance in the major malaria vector Anopheles gambiae, which we use to test the association of these markers with resistance and to study their geographic distribution. We screened resistancephenotyped An. gambiae from populations from a wide swathe of Sub-Saharan Africa (Burkina Faso, Ghana, Democratic Republic of Congo (DRC) and Kenya), and found evidence of resistance association for four mutations, including a novel mutation in the detoxification gene Gste2 (Gste2-119V). We also identified a gene duplication in Gste2 which combines a resistance-associated mutant form of the gene with its wild-type counterpart, potentially alleviating the costs of resistance. Finally, we describe the distribution of the multiple evolutionary origins of kdr resistance, finding unprecedented levels of diversity in the DRC. This panel represents the first step towards developing a quantitative predictive genotypic model of insecticide resistance that can be used to screen An. gambiae populations and predict resistance status. . revealed a heterologous duplication in Gste2 that combines resistance-associated and wild-type alleles, 2. identified an unprecedented diversity of kdr genetic backgrounds in the DRC, 3. reported the first evidence of a role for the Gste2-119V mutation in insecticide resistance in An. gambiae. 4. found evidence that a mutation found alongside kdr reduces insecticide resistance, possibly indicating that this mutation serves to compensate for physiological costs of kdr.

show abstract

Existence of the Rdl mutant alleles among the Anopheles malaria vector in Indonesia

Cited by 4 publications

References 3 publications

Chromosomal inversions and ecotypic differentiation in Anopheles gambiae: the perspective from whole‐genome sequencing

Chromosomal inversions and ecotypic differentiation in Anopheles gambiae: the perspective from whole‐genome sequencing

High-throughput barcoding method for the genetic surveillance of insecticide resistance and species identification in Anopheles gambiae complex malaria vectors

A high throughput multi-locus insecticide resistance marker panel for tracking resistance emergence and spread inAnopheles gambiae

Contact Info

Product

Resources

About