Existence of yeast genomic fragments hybridizing with the sea urchin histone H1 gene.

Miloshev, George; Zlatanova, Jordanka

doi:10.2323/jgam.40.359

Cited by 4 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, a differential effect on transcription was also observed by Linder and Thoma (67) who reported that the overexpression of the sea urchin H1␣ protein in yeast repressed polymerase I-transcribed rRNA genes and the polymerase II-transcribed ACT1 and URA3 genes but not the polymerase II-transcribed Ty gene. Two previous studies have shown that expression of an exogenous H1 in yeast at a stoichiometry well below that of the core histones resulted in a marked decrease in cell viability (23,67). The evident interpretation of this lethality is that the overexpression of H1 at moderate levels results in an excess of linker histones in yeast that already contains Hho1p.…”

Section: Discussionmentioning

confidence: 99%

The Biochemical and Phenotypic Characterization of Hho1p, the Putative Linker Histone H1 of Saccharomyces cerevisiae

Patterton¹,

Landel²,

Landsman³

et al. 1998

Journal of Biological Chemistry

143

135

View full text Add to dashboard Cite

There is currently no published report on the isolation and definitive identification of histone H1 in Saccharomyces cerevisiae. We showed that purified recombinant Hho1p (rHho1p) has electrophoretic and chromatographic properties similar to linker histones. The protein forms a stable ternary complex with a reconstituted core di-nucleosome in vitro at molar rHho1p:core ratios up to 1. Reconstitution of rHho1p with H1-stripped chromatin confers a kinetic pause at ϳ168 base pairs in the micrococcal nuclease digestion pattern of the chromatin. These results strongly suggest that Hho1p is a bona fide linker histone. We deleted the HHO1 gene and showed that the strain is viable and has no growth or mating defects. Hho1p is not required for telomeric silencing, basal transcriptional repression, or efficient sporulation. Unlike core histone mutations, a hho1⌬ strain does not exhibit a Sin or Spt phenotype. The absence of Hho1p does not lead to a change in the nucleosome repeat length of bulk chromatin nor to differences in the in vivo micrococcal nuclease cleavage sites in individual genes as detected by primer extension mapping.

show abstract

Section: Discussionmentioning

confidence: 99%