Exo-Metabolites of Phaseolus vulgaris-Nodulating Rhizobial Strains

Montes-Grajales, Diana; Esturau‐Escofet, Nuria; Esquivel, Baldomero; Martı́nez-Romero, Esperanza

doi:10.3390/metabo9060105

Cited by 10 publications

(3 citation statements)

References 73 publications

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“…In Salmonella, YigM upregulates the cysteine regulon (VanDrisse and Escalante-Semerena, 2018) and it would be interesting to further determine if Ch24-10 produces and excretes cysteine in plant roots. An exo-metabolome analysis revealed that Ch24-10 can excrete amino acids such as aspartic acid, alanine, tyrosine, and phenylalanine when it grows in MM (Montes-Grajales et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Responses of Rhizobium phaseoli to Root Exudates Reflect Its Capacity to Colonize Maize and Common Bean in an Intercropping System

et al. 2021

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Corn and common bean have been cultivated together in Mesoamerica for thousands of years in an intercropping system called “milpa,” where the roots are intermingled, favoring the exchange of their microbiota, including symbionts such as rhizobia. In this work, we studied the genomic expression of Rhizobium phaseoli Ch24-10 (by RNA-seq) after a 2-h treatment in the presence of root exudates of maize and bean grown in monoculture and milpa system under hydroponic conditions. In bean exudates, rhizobial genes for nodulation and degradation of aromatic compounds were induced; while in maize, a response of genes for degradation of mucilage and ferulic acid was observed, as well as those for the transport of sugars, dicarboxylic acids and iron. Ch24-10 transcriptomes in milpa resembled those of beans because they both showed high expression of nodulation genes; some genes that were expressed in corn exudates were also induced by the intercropping system, especially those for the degradation of ferulic acid and pectin. Beans grown in milpa system formed nitrogen-fixing nodules similar to monocultured beans; therefore, the presence of maize did not interfere with Rhizobium–bean symbiosis. Genes for the metabolism of sugars and amino acids, flavonoid and phytoalexin tolerance, and a T3SS were expressed in both monocultures and milpa system, which reveals the adaptive capacity of rhizobia to colonize both legumes and cereals. Transcriptional fusions of the putA gene, which participates in proline metabolism, and of a gene encoding a polygalacturonase were used to validate their participation in plant–microbe interactions. We determined the enzymatic activity of carbonic anhydrase whose gene was also overexpressed in response to root exudates.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Responses of Rhizobium phaseoli to Root Exudates Reflect Its Capacity to Colonize Maize and Common Bean in an Intercropping System

et al. 2021

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“…Some plants have enhanced concentrations as a result of colonization by AMF ( Schubert et al, 1992 ) and N-fixing rhizobia ( Streeter, 1985 ). By recording the 1 HNMR spectra of extracellular cultures, Montes-Grajales et al (2019) identified trehalose in a culture supernatant of N-fixing rhizobial strains R. etli CFN42 and Rhizobium leguminosarum phaseoli Ch24-10. Under symbiotic conditions, trehalose is synthesized by the bacteroid in the nodule; however, the majority of trehalose is localized in the cytoplasm of host plant cells ( Farias-Rodriguez et al, 1998 ).…”

Section: Rhizobia-mediated Trehalose Metabolism and Its Role In Abiotmentioning

confidence: 99%

Deciphering the Role of Trehalose in Tripartite Symbiosis Among Rhizobia, Arbuscular Mycorrhizal Fungi, and Legumes for Enhancing Abiotic Stress Tolerance in Crop Plants

et al. 2020

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Drought is a critical factor limiting the productivity of legumes worldwide. Legumes can enter into a unique tripartite symbiotic relationship with root-nodulating bacteria of genera Rhizobium, Bradyrhizobium, or Sinorhizobium and colonization by arbuscular mycorrhizal fungi (AMF). Rhizobial symbiosis provides nitrogen necessary for growth. AMF symbiosis enhances uptake of diffusion-limited nutrients such as P, Zn, Cu, etc., and also water from the soil via plant-associated fungal hyphae. Rhizobial and AMF symbioses can act synergistically in promoting plant growth and fitness, resulting in overall yield benefits under drought stress. One of the approaches that rhizobia use to survive under stress is the accumulation of compatible solutes, or osmolytes, such as trehalose. Trehalose is a non-reducing disaccharide and an osmolyte reported to accumulate in a range of organisms. High accumulation of trehalose in bacteroids during nodulation protects cells and proteins from osmotic shock, desiccation, and heat under drought stress. Manipulation of trehalose cell concentrations has been directly correlated with stress response in plants and other organisms, including AMF. However, the role of this compound in the tripartite symbiotic relationship is not fully explored. This review describes the biological importance and the role of trehalose in the tripartite symbiosis between plants, rhizobia, and AMF. In particular, we review the physiological functions and the molecular investigations of trehalose carried out using omics-based approaches. This review will pave the way for future studies investigating possible metabolic engineering of this biomolecule for enhancing abiotic stress tolerance in plants.

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“…Phaseolus vulgaris L. (Fabaceae) is a widely used legume for human nutrition and can form symbiotic associations with arbuscular mycorrhizal fungi (AMF) and nitrogenfixing rhizobacteria (NFR) [1]. Among the bacterial species that form root nodules in P. vulgaris, Rhizobium etli, Rhizobium phaseoli and Rhizobium tropici have been extensively studied [2,3]. R. tropici has outstanding stress resistance [4] and has been successfully used as a P. vulgaris inoculant in South America [5].…”

Section: Introductionmentioning

confidence: 99%

Rhizobium tropici and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in Phaseolus vulgaris L.

et al. 2023

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Phaseolus vulgaris L. (Leguminosae) forms symbioses with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing rhizobia (NFB). The tripartite relationship uses molecular singals to establish intracellular symbioses in roots. The goal of this study was to determine if Rhizobium tropici CIAT 899 and exogenous riboflavin (vitamin B2) have an effect on AMF species selection and root colonization of P. vulgaris. Using SSU rRNA fragment amplification of DNA extracted from P. vulgaris roots, we found that the presence of R. tropici altered the relative distribution of AMF species. Dominikia bernensis (Ohel) was the most abundant AMF species in P. vulgaris roots but when R. tropici was co-inoculated, Glomus species dominated. Rhizobacteria such as R. tropici, secrete riboflavin and could affect AMF symbiosis. Addition of 50 μM riboflavin to P. vulgaris, increased plant growth (28%), dry nodule weight (18%), AMF colonization (248%) and mycorrhizal vesicle frequency (56%) in bean roots. 3.12 and 12.5 µM riboflavin favored the presence of Glomus macrocarpum in P. vulgaris roots. This work provides de basis for further investigation of rhizobial and mycorrhizal co-inoculation of Phaseolus vulgaris bean.

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Exo-Metabolites of Phaseolus vulgaris-Nodulating Rhizobial Strains

Cited by 10 publications

References 73 publications

Transcriptomic Responses of Rhizobium phaseoli to Root Exudates Reflect Its Capacity to Colonize Maize and Common Bean in an Intercropping System

Transcriptomic Responses of Rhizobium phaseoli to Root Exudates Reflect Its Capacity to Colonize Maize and Common Bean in an Intercropping System

Deciphering the Role of Trehalose in Tripartite Symbiosis Among Rhizobia, Arbuscular Mycorrhizal Fungi, and Legumes for Enhancing Abiotic Stress Tolerance in Crop Plants

Rhizobium tropici and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in Phaseolus vulgaris L.

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