2004
DOI: 10.1677/joe.1.05656
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Exocytosis sensitivity to growth hormone-releasing hormone in subsets of GH cells in rats under different corticosterone conditions. Ultrastructural study using microwave irradiation for fixation and immunocytochemistry

Abstract: Growth hormone (GH) cells in the rat anterior pituitary have been morphologically classified into three subtypes: type I (mature) containing large secretory granules about 350 nm in diameter, type II (intermediate) containing a mixture of large and small granules, and type III (immature) containing small granules about 150 nm in diameter. However, the functional implications of morphological heterogeneity, especially the different sensitivities to growth hormone-releasing hormone (GRH) under different corticos… Show more

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Cited by 10 publications
(4 citation statements)
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“…Interestingly, in pituitaries from PICK1-deficient mice, the GH staining appeared less intense and less well organized, with no or only very few cells showing the WT pattern (Figure 5A). Electron microscopy confirmed the difference in intracellular structure of the GH-producing cells (identified by their characteristic architecture [30]). In the PICK1-deficient GH cells, we observed a reduction in the number of secretory dense core vesicles (DCVs) compared to WT cells (Figure 5B–C).…”
Section: Resultsmentioning
confidence: 60%
“…Interestingly, in pituitaries from PICK1-deficient mice, the GH staining appeared less intense and less well organized, with no or only very few cells showing the WT pattern (Figure 5A). Electron microscopy confirmed the difference in intracellular structure of the GH-producing cells (identified by their characteristic architecture [30]). In the PICK1-deficient GH cells, we observed a reduction in the number of secretory dense core vesicles (DCVs) compared to WT cells (Figure 5B–C).…”
Section: Resultsmentioning
confidence: 60%
“…Specimens for electron microscopic observation were prepared as described previously with slight modification ( 46 ). Briefly, under deep anesthesia, mice were perfused through the left cardiac ventricle with 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) followed by physiological saline.…”
Section: Methodsmentioning
confidence: 99%
“…Recent technological advances in microwave irradiation devices have also allowed greater control of the irradiation level, irradiation time, and intermittent microwave irradiation (Kumada et al 2004, Temel et al 2005, 2006; therefore, microwave irradiation should be applicable for many histological techniques including immunostaining and electron microscopy (Cavuşog lu et al 1998, Kahveci et al 2003, Koga et al 2003, Ozawa et al 2004). We established a method for rapid fixation and immunofluorescence microscopy using intermittent microwave irradiation.…”
Section: Discussionmentioning
confidence: 99%