Exogenous 20-hydroxyecdysone induces epidermal carbonic anhydrase but inhibits exoskeletal calcification in the post-ecdysial blue crab, Callinectes sapidus

Ostrowski, Andria; Zou, Enmin

doi:10.1016/j.ygcen.2018.07.018

Cited by 7 publications

(3 citation statements)

References 30 publications

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“…For instance, Pb could act through swaying hormonal signaling that regulates postecdysial mineralization, thereby suppressing exoskeletal calcification. Currently, two hormones have been implicated in the regulation of postecdysial shell hardening in crustaceans, with the neurohormone bursicon controlling sclerotization (Chung et al, 2012), while the ecdysteroid molting hormone appears to play a role in regulating mineralization (Ostrowski & Zou, 2018). The fact that Pb treatment had no effect on carbonic anhydrase activity in the epidermis suggests that Pb does not impact ecdysteroid signaling because Ostrowski and Zou (2018) demonstrated that the expression of epidermal carbonic anhydrase is under the control of the molting hormone.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, two hormones have been implicated in the regulation of postecdysial shell hardening in crustaceans, with the neurohormone bursicon controlling sclerotization (Chung et al, 2012), while the ecdysteroid molting hormone appears to play a role in regulating mineralization (Ostrowski & Zou, 2018). The fact that Pb treatment had no effect on carbonic anhydrase activity in the epidermis suggests that Pb does not impact ecdysteroid signaling because Ostrowski and Zou (2018) demonstrated that the expression of epidermal carbonic anhydrase is under the control of the molting hormone. However, Pb could still act through impacting bursicon signaling in the epidermis, presuming that bursicon is involved in controlling the expression of epidermal calcium transporters in addition to regulating sclerotization.…”

Section: Discussionmentioning

confidence: 99%

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Lead Inhibits Postecdysial Exoskeletal Calcification in the Blue Crab (Callinectes sapidus)

Osayande

Zou

2022

Enviro Toxic and Chemistry

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Postecdysial mineralization in crustaceans involves the deposition of carbonate salts, such as calcium carbonate, to the organic matrix. Because of the resemblance between Pb2+ and Ca2+, the present study was carried out to investigate whether Pb is incorporated into the new shell during postecdysial mineralization using the blue crab (Callinectes sapidus) as the model crustacean. It was hypothesized that injected Pb would be deposited in the shell via calcium transporters in the epidermis during the mineralization process. Postecdysial blue crabs were injected with two doses of 5 µg Pb/g wet weight each in lead acetate, and then Pb, Ca, and Mg contents were analyzed in the exoskeleton, while only Pb bioaccumulation was quantified for the hepatopancreas, gills, muscles, and hemolymph. The results showed a statistically nonsignificant increase in exoskeletal Pb content in Pb‐treated crabs compared to control, suggesting that exoskeletal Pb may not be a sensitive proxy for aquatic Pb pollution. There was a significant decrease in Ca content in Pb‐treated crabs, suggesting that Pb hindered the deposition of Ca to crab exoskeleton, thereby obstructing calcification. A trend of a decrease in exoskeletal Mg was also observed in Pb‐treated crabs. There was a significant increase in Pb content found in the gills, hepatopancreas, muscle, and hemolymph in Pb‐treated crabs. The rank of the Pb level among three soft tissues in a decreasing order is hepatopancreas > gill > muscle. This is the first study to present evidence that Pb disrupts postecdysial exoskeletal calcification in a crustacean. Environ Toxicol Chem 2022;41:474–482. © 2021 SETAC

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Lead Inhibits Postecdysial Exoskeletal Calcification in the Blue Crab (Callinectes sapidus)

Osayande

Zou

2022

Enviro Toxic and Chemistry

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“…The protocols for metal quantification essentially followed those of Ostrowski & Zou (2018), Butler & Zou (2021), and Osayande & Zou (2022). Briefly, each carapace sample was prepared by drying the carapace in a Fisherbrand Scientific Isotemp oven at 50 °C until a constant weight was measured.…”

Section: Methodsmentioning

confidence: 99%

How Does Carbon Dioxide–Induced Acidification Affect Postecdysial Exoskeletal Mineralization in the Blue Crab (Callinectes sapidus)?

Soorya

Zou

2022

Enviro Toxic and Chemistry

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Carbon dioxide (CO2) enrichment in seawater because of increased use of fossil fuels can possibly cause detrimental effects on the physiological processes of marine life, especially shell builders, due to CO2‐induced ocean acidification. We investigated, for the first time, specifically the effect of CO2 enrichment on postecdysial shell mineralization in Crustacea using the blue crab, Callinectes sapidus, as the model crustacean. It was hypothesized that CO2 enrichment of seawater would adversely affect exoskeletal mineralization in the blue crab. We used two groups of postecdysial crabs, with one group exposed to seawater at a pH of 8.20 and the other group treated with CO2‐acidified seawater with a pH of 7.80–7.90. After a period of 7 days, samples of exoskeleton and hemolymph were collected from the survivors. Enrichment was found to significantly increase exoskeletal magnesium content by 104% relative to control, whereas a statistically nonsignificant elevation of 31% in exoskeletal calcium was registered. Because CO2 treatment did not change the content of magnesium and calcium in the hemolymph, we postulate that increased exoskeletal mineralization in postecdysial blue crabs must stem from an increased influx of bicarbonate ions from the medium through the gill, to the hemolymph, and across the epidermis. In addition, the observed significant increase in the mass of exoskeleton following CO2 treatment must be at least partly accounted for by enhanced postmolt carbonate salt deposition to the shell. Environ Toxicol Chem 2022;41:2950–2954. © 2022 SETAC

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Aquatic invertebrate endocrine disruption

Zou

2024

Reference Module in Life Sciences

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Exogenous 20-hydroxyecdysone induces epidermal carbonic anhydrase but inhibits exoskeletal calcification in the post-ecdysial blue crab, Callinectes sapidus

Cited by 7 publications

References 30 publications

Lead Inhibits Postecdysial Exoskeletal Calcification in the Blue Crab (Callinectes sapidus)

Lead Inhibits Postecdysial Exoskeletal Calcification in the Blue Crab (Callinectes sapidus)

How Does Carbon Dioxide–Induced Acidification Affect Postecdysial Exoskeletal Mineralization in the Blue Crab (Callinectes sapidus)?

Aquatic invertebrate endocrine disruption

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