Exogenous 3,3′-diindolylmethane increases Brassica napus L. seedling shoot growth through modulation of superoxide and hydrogen peroxide content

Gokul, Arun; Roode, Enrico; Klein, Ashwil; Keyster, Marshall

doi:10.1016/j.jplph.2016.03.013

Cited by 18 publications

(20 citation statements)

References 43 publications

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“…•− plays a crucial role in the downstream signalling mechanism of p-CA in chia seedlings. This hypothesis was also observed by [19] where increases in O 2…”

Section: Discussionsupporting

confidence: 71%

“…The shoots from each treatment were scored for length (SL), fresh weight (FW) and dry weight (DW). The DW was determined by drying the seedlings in an oven at 55 • C for 48 h as described by [19].…”

Section: Measurement Of Plant Growthmentioning

confidence: 99%

“…•− ) content in the shoots of chia seedlings was quantified using a method previously described in [19]. Superoxide concentrations were determined by submerging intact seedling shoots in a solution containing; 10 mM KCN (to inhibit Cu/Zn SODs), 10 mM H 2 O 2 (to inhibit Mn and Cu/Zn SODs), 2% (w/v) SDS (to inhibit Mn and Fe SODs), 80 mM Nitroblue tetrazolium chloride (NBT) and 50 mM potassium phosphate (pH 7.0) and incubated for 20 min.…”

Section: Superoxide (Omentioning

confidence: 99%

“…[17,18]. Furthermore, a study by Gokul et al [19] showed that O 2 •− and H 2 O 2 regulate physio-biochemical responses in Brassica napus plants via the modulation of SOD and APX activity, which in turn led to increased seedling growth. This evidence suggests that the mechanism controlling redox homeostasis of ROS molecules can be exploited to improve plant growth.…”

Section: Introductionmentioning

confidence: 99%

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Exogenous p-Coumaric Acid Improves Salvia hispanica L. Seedling Shoot Growth

Nkomo

Gokul

Keyster

et al. 2019

Plants

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: p-Coumaric acid (p-CA) belongs to a family of natural esters of hydroxycinnamic acid compounds that have been shown to modulate plant growth and metabolism. In this study, we investigated the effect of exogenous p-CA on plant growth, reactive oxygen species (ROS)-induced oxidative damage, photosynthetic metabolism, osmolyte content and changes in superoxide dismutase (SOD) enzymatic activity. Exogenous p-CA improved Salvia hispanica (chia) growth by significantly enhancing shoot length, fresh and dry weights coupled with augmented levels of total chlorophyll and carotenoid contents. Furthermore, p-CA also triggered an induction in proline, glycine betaine (GB) and superoxide (O2∙−) levels while no changes were observed for hydrogen peroxide (H2O2) and downstream malondialdehyde (MDA) content. Also, no change in SOD activity was observed in the p-CA treatment relative to the control. Therefore, the results suggest that exogenous p-CA improves chia seedling growth possibly via activation of a ROS-signalling pathway involving O2∙− under the control of proline accumulation.

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“…•− plays a crucial role in the downstream signalling mechanism of p-CA in chia seedlings. This hypothesis was also observed by [19] where increases in O 2…”

Section: Discussionsupporting

confidence: 71%

Section: Measurement Of Plant Growthmentioning

confidence: 99%

Section: Superoxide (Omentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exogenous p-Coumaric Acid Improves Salvia hispanica L. Seedling Shoot Growth

Nkomo

Gokul

Keyster

et al. 2019

Plants

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“…On the other hand, there is the possibility of enhanced cellular responses due to the applied AGE and ASA treatments, which may have activated the antioxidant enzymes' activity. The involvement of ROS in the plant developmental processes, particularly in the polysaccharide metabolism and cell wall loosening and elongation [62][63][64], further indicates the possibility of growth promotion in the current treated seedlings. ROS have been shown to promote root development [65], and in the current study, it was observed that upon application of the treatments, plant roots were significantly improved, particularly in the AGE treated plants.…”

Section: Application Of Age-improved Photosynthetic Pigments Solublementioning

confidence: 86%

Aqueous Garlic Extract as a Plant Biostimulant Enhances Physiology, Improves Crop Quality and Metabolite Abundance, and Primes the Defense Responses of Receiver Plants

et al. 2018

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Biostimulants are the next-generation choice for sustainable agricultural production and are gradually becoming an alternative to synthetic chemicals. Various botanicals are proposed to exert stimulatory effects, and garlic allelochemicals are among such botanicals; however, a peer-reviewed scientific evaluation is required to understand garlic-derived substances such as biostimulants. Current studies were therefore performed to identify the bioactivity of garlic extract as a biostimulant to improve crop quality, alter its physiological potential, and prime its defense responses against pathogenic fungal infections. 100 µg mL−1 aqueous garlic extracts (AGE) in consort with 1 mM of acetyl salicylic acid (ASA) and distilled water as a control treatment were applied to eggplant and pepper seedlings as foliar application and fertigation methods. The results revealed stimulatory responses in the growth of the vegetables with improved plant height, number of leaves, root growth, fresh and dry weight, etc., due to AGE and ASA applications. Moreover, significant alterations were indicated in plant metabolites such as chlorophyll, carotenoids, and soluble sugars. Additionally, stimulation of the antioxidant enzymes such as superoxide dismutase (SOD) and peroxidase (POD), as well as the root activity of these plants, was observed after treatment. Application of AGE and ASA also exerted priming effects on pepper plants, inducing defense responses prior to Phytopthora capsici inoculation, and the treated plants therefore successfully resisted infection through activated antioxidant systems, and probably carotenoid and other protectory metabolites. Stress-induced H2O2 content was extremely low in the treated plants, indicating successful resistance against pathogenic infection.

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