Exogenous abscisic acid reduces saikosaponin accumulation by inhibiting saikosaponin synthesis pathway gene expression under drought stress in Bupleurum chinense DC.

Yang, Linlin; Yang, Li; Lan, Yi-ming; Yu, Zujiang; Han, Mei; Yang, Limin

doi:10.1016/j.indcrop.2020.112686

Cited by 15 publications

(9 citation statements)

References 37 publications

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“…A qRT-PCR analysis was completed using an Mx 3000P instrument (Agilent, Santa Clara, CA, USA). The primers for each gene reaction procedure have been previously reported [ 17 ]. Each 20 μL PCR contained 10 μL of 2 × SYBR Premix Ex Taq, and the optimum amounts of primers and cDNA were 0.8 μL and 1.0 μL, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Saikosaponin biosynthesis includes three stages: (1) the glycolytic product acetyl-CoA is converted into the precursors 3-isoprene pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) via the mevalonic acid pathway (MVA) [ 12 ]; (2) IPP and DMAPP are catalyzed into 2,3-oxidized squalene via various enzymes and intermediates [ 13 ]; (3) various monomer saikosaponins are synthesized via cyclization, hydroxylation, and glycosylation of 2,3-oxidized squalene [ 14 , 15 ]. However, the genes involved in saikosaponin biosynthesis are not fully characterized, and their regulation by external ecological factors remains unclear [ 16 , 17 ]. Therefore, understanding the secondary metabolic process of saikosaponin synthesis and the mechanism underlying its molecular regulation in terms of the intrinsic gene expression and external ecological factors (drought) remains essential.…”

Section: Introductionmentioning

confidence: 99%

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Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in Bupleurum chinense DC. Roots

Yang

Qiao

et al. 2022

Molecules

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Bupleurum chinense is an important medicinal plant in China; however, little is known regarding how this plant transcribes and synthesizes saikosaponins under drought stress. Herein, we investigated how drought stress stimulates the transcriptional changes of B. chinense to synthesize saikosaponins. Short-term drought stress induced the accumulation of saikosaponins, especially from the first re-watering stage (RD_1 stage) to the second re-watering stage (RD_2 stage). Saikosaponin-a and saikosaponin-d increased by 84.60% and 75.13%, respectively, from the RD_1 stage to the RD_2 stage. Drought stress also stimulated a rapid increase in the levels of the hormones abscisic acid, salicylic acid, and jasmonic acid. We screened 49 Unigenes regarding the terpenoid backbone and triterpenoid biosynthesis, of which 33 differential genes were significantly up-regulated during drought stress. Moreover, one P450 and two UGTs are possibly involved in the synthesis of saikosaponins, while some transcription factors may be involved in regulating the expression of key enzyme genes. Our study provides a reference for the cultivation of B. chinense and a practical means to ensure the quality (safety and effectiveness) of B. chinense for medicinal use, as well as insights into the modernization of the China Agriculture Research System.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in Bupleurum chinense DC. Roots

Yang

Qiao

et al. 2022

Molecules

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“…A complex signaling network integrating phytohormones and metabolites regulates the biosynthesis of secondary metabolites in plants (Wang and Wu, 2005;Karppinen et al, 2016). Jasmonate (JA), abscisic acid (ABA), salicylic acid (SA), and gibberellic acids (GAs) are important plant hormones involved in the biosynthesis and accumulation of bioactive secondary metabolites in medicinal plants (Gao et al, 2012;Liang et al, 2013;Afrin et al, 2015;Yang et al, 2020). The exogenous application of these stimuli regulated the expression of genes/proteins in the biosynthetic pathways of secondary metabolites (Lee et al, 2008;Kiselev et al, 2010;Yang et al, 2020).…”

Section: Signal Molecules Involved In Sulfur-regulated Andrographolide Biosynthesismentioning

confidence: 99%

“…Jasmonate (JA), abscisic acid (ABA), salicylic acid (SA), and gibberellic acids (GAs) are important plant hormones involved in the biosynthesis and accumulation of bioactive secondary metabolites in medicinal plants (Gao et al, 2012;Liang et al, 2013;Afrin et al, 2015;Yang et al, 2020). The exogenous application of these stimuli regulated the expression of genes/proteins in the biosynthetic pathways of secondary metabolites (Lee et al, 2008;Kiselev et al, 2010;Yang et al, 2020). It has been reported that MeJA induced andrographolide biosynthesis by the upregulation of genes in the MVA and MEP pathways (Sharma et al, 2015;Gao et al, 2019;Sun et al, 2019).…”

Section: Signal Molecules Involved In Sulfur-regulated Andrographolide Biosynthesismentioning

confidence: 99%

Sulfur Regulates the Trade-Off Between Growth and Andrographolide Accumulation via Nitrogen Metabolism in Andrographis paniculata

et al. 2021

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Nitrogen (N) and sulfur (S) are essential mineral nutrients for plant growth and metabolism. Here, we investigated their interaction in plant growth and andrographolide accumulation in medicinal plant Andrographis paniculata grown at different N (4 and 8 mmol·L−1) and S concentration levels (0.1 and 2.4 mmol L−1). We found that increasing the S application rate enhanced the accumulation of andrographolide compounds (AGCs) in A. paniculata. Simultaneously, salicylic acid (SA) and gibberellic acid 4 (GA4) concentrations were increased but trehalose/trehalose 6-phosphate (Tre/Tre6P) concentrations were decreased by high S, suggesting that they were involved in the S-mediated accumulation of AGCs. However, S affected plant growth differentially at different N levels. Metabolite analysis revealed that high S induced increases in the tricarboxylic acid (TCA) cycle and photorespiration under low N conditions, which promoted N assimilation and S metabolism, and simultaneously increased carbohydrate consumption and inhibited plant growth. In contrast, high S reduced N and S concentrations in plants and promoted plant growth under high N conditions. Taken together, the results indicated that increasing the S application rate is an effective strategy to improve AGC accumulation in A. paniculata. Nevertheless, the interaction of N and S affected the trade-off between plant growth and AGC accumulation, in which N metabolism plays a key role.

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“…For example, exogenous ABA at an appropriate concentration can promote the accumulation of the main bioactive components (glycyrrhizin, liquiritin, isoliquiritin, liquiritigenin and isoliquiritigenin) in Glycyrrhiza uralensis [ 22 ]. Exogenous ABA can also regulate saikosaponin synthesis of Bupleurum chinense under drought stress [ 23 ]. Other researchers found that after induction by JA, callus cultures of Hibicus sabdariffa accumulated more bioactive compounds and showed stronger antioxidant activity [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Study on the Regulation of Exogenous Hormones on the Absorption of Elements and the Accumulation of Secondary Metabolites in the Medicinal Plant Artemisia argyi Leaves

et al. 2022

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As an important medicinal plant, we still do not know the effect of exogenous hormones on absorption of elements and accumulation of secondary metabolites in Artemisia argyi leaves. In this work, we analyzed the difference in 21 elements absorbed by A. argyi leaves under three exogenous hormone (MeJA, SA and ABA) treatments, and also clarified the correlation between 21 elements and eight bioactive components. Different hormone treatments changed the absorption and enrichment of elements, and the composition also changed significantly. The contents of eight bioactive components changed significantly under different hormone treatments. When A. argyi was stimulated by exogenous hormones, the content of secondary metabolites was adjusted in the leaves through changes in the absorption and enrichment of elements. The widely untargeted metabolomic analysis further confirmed that ABA changes the metabolic direction of secondary metabolites in A. argyi leaves and stimulates the biosynthesis of multiple secondary metabolites including phenylpropanoids, flavonoids, terpenoids, alkaloids and others. These results provide a new perspective for the changes in element absorption and the mechanism of secondary metabolic components in A. argyi leaves under exogenous hormone treatments, and also deepen people’s understanding of the interaction mechanism between medicinal plants and hormones.

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Exogenous abscisic acid reduces saikosaponin accumulation by inhibiting saikosaponin synthesis pathway gene expression under drought stress in Bupleurum chinense DC.

Cited by 15 publications

References 37 publications

Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in Bupleurum chinense DC. Roots

Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin in Bupleurum chinense DC. Roots

Sulfur Regulates the Trade-Off Between Growth and Andrographolide Accumulation via Nitrogen Metabolism in Andrographis paniculata

Study on the Regulation of Exogenous Hormones on the Absorption of Elements and the Accumulation of Secondary Metabolites in the Medicinal Plant Artemisia argyi Leaves

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