Exogenous addition of indole acetic acid and kinetin under nitrogen-limited medium enhances lipid yield and expression of glycerol-3-phosphate acyltransferase &amp; diacylglycerol acyltransferase genes in indigenous microalgae: A potential approach for biodiesel production

Mandal, Mrinal Kanti; Chanu, Ng. Kunjarani; Chaurasia, Neha

doi:10.1016/j.biortech.2019.122439

Cited by 38 publications

(6 citation statements)

References 43 publications

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“…In this study, 5 mg/L IAA, 10 mg/L IAA, 10 mg/L ABA and a combination of IAA and ABA (each 5 mg/L) were found to promote the FACHB-8 cell growth. The effects of IAA and ABA concentrations on microalgae cell growth are controversial [ 28 , 29 , 30 ]. This may be caused by the differences in microalgae species, strains, and culture conditions, including light intensity, light period, and medium element concentrations.…”

Section: Discussionmentioning

confidence: 99%

The Growth, Lipid Accumulation and Fatty Acid Profile Analysis by Abscisic Acid and Indol-3-Acetic Acid Induced in Chlorella sp. FACHB-8

Lin

Dai

et al. 2022

IJMS

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Microalgae are considered a promising source for biodiesel. The addition of plant hormone can exert a significant impact on the production of microalgae biomass and lipid accumulation. Nevertheless, the response of microalgae cells to hormones is species- or strain-dependent. It remains controversial which genes involved in strong increase of fatty acids production in response to abscisic acid (ABA) in Chlorella sp. FACHB-8 strain. We investigated cell growth, lipid accumulation, and fatty acid composition when ABA and indol-3-acetic acid (IAA) were used in the growth medium of Chlorella sp. FACHB-8. The four treatments, including 5 mg/L IAA (E1), 10 mg/L IAA (E2), 10 mg/L ABA (E3), the combination of 5 mg/L IAA and 5 mg/L ABA (E4), were found to increase cell growth, but only 10 mg/L ABA treatment could enhance the lipid accumulation. The fatty acid profile was changed by the addition of ABA, making fatty acids afflux from polyunsaturated fatty acids to monounsaturated and saturated fatty acids, which were suitable for diesel application. Furthermore, a transcriptome analysis was conducted, unraveling the differentially expressed genes enriched in fatty acid biosynthesis, fatty acid metabolism, and biosynthesis of the unsaturated fatty acid pathway in response to ABA. Our results clarified the correlation of fatty acid synthesis-related genes and fatty acid profiles, helping understand the potential response mechanism of Chlorella sp. FACHB-8 strain respond to ABA treatment.

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Section: Discussionmentioning

confidence: 99%

The Growth, Lipid Accumulation and Fatty Acid Profile Analysis by Abscisic Acid and Indol-3-Acetic Acid Induced in Chlorella sp. FACHB-8

Lin

Dai

et al. 2022

IJMS

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“…In another work, Mandal et al (2020) induced the expression of acyltransferases in Graesiella emersonii NC-M1 and Chlorophyta sp. NC-M5 by the addition of phytohormones under nitrogenlimited (NL) conditions.…”

Section: Biodiesel Production Using Other Classes Of Enzymesmentioning

confidence: 92%

Novel concepts for the biocatalytic synthesis of second-generation biodiesel

Spanou,

Moschona,

Theodosiou

et al. 2024

Front. Catal.

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Biodiesel is synthesized by the transesterification of triglycerides of oils with short-chain alcohols, such as methanol and ethanol. According to the Renewable Energy Directive guidelines (RED II 2018/2001/EU) the contribution of advanced biofuels, which do not include edible oils, towards the overall EU target, is at 1% in 2025 and at least 3.5% in 2030. Bioprocesses that valorize non-edible oils for the production of second-generation biodiesel could play a critical role in achieving this goal. Immobilized lipases, as well as other enzyme classes, such as cutinases and acyltransferases, are utilized as biocatalysts for this process. For the sustainability of the process, renewable materials can be used as immobilization matrices, or even enzymes anchored on the cells as whole-cell biocatalysts. Membrane reactors can also be employed to facilitate the enzymatic transesterification by conducting a continuous enzymatic reaction and simultaneously separate the products in a single operation. The advances on the aforementioned fast-pacing fields are presented in this work.

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“…Plant regulators were usually used for lipid induction. Treatment with salicylic acid induced significantly higher lipid and EPA production in Nannochloropsis oceanica [64], while a combination of indole acetic acid and kinetin achieved a 2.3-and 2.5-fold increase in biomass and lipid yield for Graesiella emersonii [65]. On the other hand, algae-associated bacteria can significantly enhance lipid production.…”

Section: Lipid Induction Strategies In Microalgaementioning

confidence: 95%

Towards Lipid from Microalgae: Products, Biosynthesis, and Genetic Engineering

Xin,

Wu,

Miao

et al. 2024

Life

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Microalgae can convert carbon dioxide into organic matter through photosynthesis. Thus, they are considered as an environment-friendly and efficient cell chassis for biologically active metabolites. Microalgal lipids are a class of organic compounds that can be used as raw materials for food, feed, cosmetics, healthcare products, bioenergy, etc., with tremendous potential for commercialization. In this review, we summarized the commercial lipid products from eukaryotic microalgae, and updated the mechanisms of lipid synthesis in microalgae. Moreover, we reviewed the enhancement of lipids, triglycerides, polyunsaturated fatty acids, pigments, and terpenes in microalgae via environmental induction and/or metabolic engineering in the past five years. Collectively, we provided a comprehensive overview of the products, biosynthesis, induced strategies and genetic engineering in microalgal lipids. Meanwhile, the outlook has been presented for the development of microalgal lipids industries, emphasizing the significance of the accurate analysis of lipid bioactivity, as well as the high-throughput screening of microalgae with specific lipids.

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Exogenous addition of indole acetic acid and kinetin under nitrogen-limited medium enhances lipid yield and expression of glycerol-3-phosphate acyltransferase & diacylglycerol acyltransferase genes in indigenous microalgae: A potential approach for biodiesel production

Cited by 38 publications

References 43 publications

The Growth, Lipid Accumulation and Fatty Acid Profile Analysis by Abscisic Acid and Indol-3-Acetic Acid Induced in Chlorella sp. FACHB-8

The Growth, Lipid Accumulation and Fatty Acid Profile Analysis by Abscisic Acid and Indol-3-Acetic Acid Induced in Chlorella sp. FACHB-8

Novel concepts for the biocatalytic synthesis of second-generation biodiesel

Towards Lipid from Microalgae: Products, Biosynthesis, and Genetic Engineering

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