Exogenous and Endogenous Mediators of Oxygen Metabolism: Alternatives for Chemical and Biological Activity

Fedorova, G. F.; Kancheva, V. D.; Menshov, Valery A.; Naumov, Vladimir V.; Vasil’ev, R. F.; Veprintsev, Timur L.; Trofimov, Aleksei V.; Tsaplev, Yu. B.; Yablonskaya, Olga I.

doi:10.1016/b978-0-444-63603-4.00011-5

Cited by 11 publications

(22 citation statements)

References 102 publications

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“…Evaluation of antioxidant activity of bioactive compounds is a novel and prospective field in biosensor application [33] , [34] . The assay systems based on luminous marine bacteria and/or their enzyme reactions are proper candidates in this field.…”

Section: Introductionmentioning

confidence: 99%

On mechanism of antioxidant effect of fullerenols

Sachkova

Kovel

Чурилов

et al. 2017

Biochemistry and Biophysics Reports

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Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic toxicants of oxidative type – 1,4-benzoquinone and potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 and mixture of two types of fullerenols С60О2–4(ОН)20–24+С70О2–4(ОН)20–24. Bacteria-based and enzyme-based bioluminescent assays were used to evaluate a decrease in cellular and biochemical toxicities, respectively. Additionally, the enzyme-based assay was used for the direct monitoring of efficiency of the oxidative enzymatic processes. The bacteria-based and enzyme-based assays showed similar peculiarities of the detoxification processes: (1) ultralow concentrations of fullerenols were active (ca 10–17–10−4 and 10–17–10−5 g/L, respectively), (2) no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and (3) detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effect of highly diluted fullerenol solutions on bacterial cells was attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. Sequence analysis of 16S ribosomal RNA was carried out; it did not reveal mutations in bacterial DNA. The suggestion was made that hydrophobic membrane-dependent processes are involved to the detoxifying mechanism. Catalytic activity of fullerenol (10−8 g/L) in NADH-dependent enzymatic reactions was demonstrated and supposed to contribute to adaptive bacterial response.

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Section: Introductionmentioning

confidence: 99%

On mechanism of antioxidant effect of fullerenols

Sachkova

Kovel

Чурилов

et al. 2017

Biochemistry and Biophysics Reports

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“…upon the abrupt change of oxygen concentration from 0 to 0.00236 m at the moment t = 0. Curves 1‐6 represent the results of modeling the chemiluminescence kinetics at the values of rate constant k 13 of reaction stage 13 (Scheme ) equal to 2.8x10 6 m −1 s −1 () and 2.8x10 7 m −1 s −1 ( 2 and 6 ) and at different ratios φ * 13 / φ * 16 of the chemiexcitation yields φ * 13 and φ * 16 in stages 13 and 16: 100 ( 1 and 2 ), 1 (), 0.1 ( 4 ), 10 (5 and 6). Symbols ○ and ■ placed on the computed curves correspond to the two sets of experimental data acquired with the two different samples of AIBN .…”

Section: Resultsmentioning

confidence: 99%

“…Mechanistic and kinetic details of the excited‐state generation in oxidation processes and peroxide reactions and of the subsequent light emission (chemi‐ and/or bioluminescence) derived from them are of prime importance from both fundamental and applied points of view. Indeed, oxidative interactions account for the occurrence of light in numerous biological systems, from ultraweak spontaneous glow of cells and tissues to bright bioluminescence of fireflies, worms and a number of marine organisms . It should also be mentioned that the effects of diverse physiologically active substances, including ingredients of drugs, are directly or indirectly related to the regulation of oxygen metabolism, which, in turn, affects the appearance of light in biologically significant oxidative processes.…”

Section: Introductionmentioning

confidence: 99%

“…However, in fact, it is more convenient to use for these purposes not the “glowing” biological processes themselves, but their chemical models and analogs, namely the chemiluminescence emission in more simple and controlled chemical reactions, which mimic in salient features the generation of light in their biochemical prototypes, but not requiring for their implementation easily deteriorating biological materials. In this context, the use of oxidation of hydrocarbon substrates followed by the light emission is very suitable and efficient . In particular, these reactions provide the most relevant basis for chemiluminescent methods for studying the activity of bioantioxidants , prominent modulators of oxygen metabolism and oxidative stress developments.…”

Section: Introductionmentioning

confidence: 99%

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Updating the Chemiluminescence Oxygen‐Aftereffect Method for Determining the Rate Constant of the Peroxy‐Radical Self‐Reaction: Oxidation of Cyclohexene

Fedorova

Lapina

Menshov

et al. 2018

Photochem & Photobiology

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Updating the facile chemiluminescence oxygen‐aftereffect method, most suitable for determining the rate constant (kt) of the peroxy‐radical self‐reaction (main chemiluminescence channel), pertained to considering the sensitivity of such a method toward a disturbing influence of the peroxy radicals of the initiator of the chain oxidation process. Such a disturbance may derive from the side chemiluminescent reaction, which involves peroxy radicals of both hydrocarbon and initiator. To examine the applicability and limitations of the chemiluminescence method under present scrutiny, cyclohexene was used as the model oxidizable hydrocarbon substrate. Computer simulations of the reaction and chemiluminescence kinetics have demonstrated the validity of the considered methodology at the value of the rate constant of the propagation of the overall chain process by peroxy radicals of the initiator higher than 1 m−1 s−1. Despite that the chemiluminescence time profile and the stationary level of the total chemiluminescence intensity depend on the kinetics of the side chemiluminescence channel and on the ratio of the excited‐state generation yields in the mentioned reaction channel and in the main chemiluminescence process, the value of kt assessed by the oxygen‐aftereffect method has been found independent of variation of these characteristics.

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“…Evaluation of antioxidant activity of bioactive compounds is a novel and prospective field in biosensor application [47][48]. The assay systems based on luminous marine bacteria and/or their enzyme reaction are proper candidates in this field.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Toxicity of Fullerenols with Different Number of Hydroxyl Substituents

Kovel¹,

Sachkova²,

Vnukova³

et al. 2018

Preprint

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Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. We studied biological effects of a series of fullerenols. Antioxidant activity and toxicity of the fullerenols were compared using bioluminescence assays (cellular and enzymatic); a content of Reactive Oxygen Species in fullerenol solutions was determined using chemiluminescence luminol method. Two groups of fullerenols with different number of hydroxyl substituents were under investigation: (I) С60Оy(OH)x, С60,70Оy(OH)x, where х+у=24–28 and (II) С60,70Оy(OH)x, Fe0,5С60Оy(OH)x, where х+у=40–42. Toxicity of the fullerenols was evaluated using effective concentrations ЕС50. Fullerenol’ antioxidant activity was investigated in model solutions of organic toxicant of oxidative type, 1,4-benzoquinone. Detoxification coefficients were calculated to analyze and compare the antioxidant activity. Higher toxicity and lower antioxidant activity were demonstrated in the solutions of fullerenols with higher number of the oxygen substituents (х+у=40–42). The differences were concerned with fullerenol’ ability to disturb Reactive Oxygen Species balance in aqueous solutions. Toxic effect of the prospective endohedral metal-fullerenol with gadolinium atom involved, Gd@C82Oy(OH)x, where х+у=40–42, was evaluated and explained by a high number of oxygen groups

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Exogenous and Endogenous Mediators of Oxygen Metabolism: Alternatives for Chemical and Biological Activity

Cited by 11 publications

References 102 publications

On mechanism of antioxidant effect of fullerenols

On mechanism of antioxidant effect of fullerenols

Updating the Chemiluminescence Oxygen‐Aftereffect Method for Determining the Rate Constant of the Peroxy‐Radical Self‐Reaction: Oxidation of Cyclohexene

Antioxidant Activity and Toxicity of Fullerenols with Different Number of Hydroxyl Substituents

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