Exogenous apelin changes alpha and beta myosin heavy chain mRNA expression and improves cardiac function in PTU-induced hypothyroid rats

Shahrivar, Farzaneh Faraji; Badavi, Mohammad; Dianat, Mahin; Mard, Ali; Ahangarpour, Akram; Samarbafzadeh, Alireza

doi:10.1016/j.gene.2016.09.025

Cited by 5 publications

(5 citation statements)

References 28 publications

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“…Another point of interest in the findings of the current study is that CsA administration significantly increased ventricular P-MHC mRNA expression and β-MHC / α-MHC ratio with no change in α-MHC mRNA expression. It has been previously reported that up-regulation of β-MHC mRNA expression caused heart failure in adult rats ( 51 52 ). Furthermore, previous observations indicated that ischemic and dilated cardiomyopathies are associated with the increased expression of β-MHC ( 53 ).…”

Section: Discussionmentioning

confidence: 99%

Moderate exercise mitigates cardiac dysfunction and injury induced by cyclosporine A through activation of the PGI2 / PPAR-γ signaling pathway

Nourmohammadi,

Bayrami,

Naderi

et al. 2023

Research in Pharmaceutical Sciences

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Background and purpose: The present study investigated the role of the prostaglandin I2/peroxisome proliferator activator receptor (PGI2/PPAR) signaling pathway in cardiac cell proliferation, apoptosis, and systemic hemodynamic variables under cyclosporine A (CsA) exposure alone or combined with moderate exercises. Experimental approach: Twenty-four male Wistar rats were classified into three groups, namely, control, CsA, and CsA + exercise. Findings/Results: After 42 days of treatment, the findings showed a significant enhancement in the expression of the β-MHC gene, enhancement in protein expression of Bax and caspase-3, and a significant decline in the protein expression of Bcl-2 expression, as well as increased proliferation intensity in the heart tissue of the CsA group compared to the control group. Systolic pressure, pulse pressure, mean arterial pressure (MAP), QT and QRS duration, and T wave amplitude, as well as QTc amount in the CsA group, showed a significant increase compared to the control group. PPAR-γ and PGI2 showed no significant changes compared to the control group. Moderate exercise along with CsA significantly enhanced the protein expression of PPAR-γ and PGI2 and declined protein expression of Bax, and caspase-3 compared to those in the CsA group. In the CsA + exercise group, systolic pressure, MAP, and Twave showed a significant decrease compared to the CsA group. Moderate exercises along CsA improved heart cell proliferation intensity and significantly reduced β- MHC gene expression compared to the CsA group. Conclusions and implications: The results showed moderate exercise alleviated CsA-induced heart tissue apoptosis and proliferation with the corresponding activation of the PGI2/PPAR-γ pathway.

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Section: Discussionmentioning

confidence: 99%

Moderate exercise mitigates cardiac dysfunction and injury induced by cyclosporine A through activation of the PGI2 / PPAR-γ signaling pathway

Nourmohammadi,

Bayrami,

Naderi

et al. 2023

Research in Pharmaceutical Sciences

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“…It has also been shown that apelin induces the phosphorylation of the myosin light chain and helps in vasoconstriction (Hashimoto et al, 2006). Apelin has also been demonstrated to increase alpha and beta myosin chain expression, resulting in improved cardiac contractility (Shahrivar et al, 2016). Thus, it could be suggested that APJ signaling might be involved in the formation of manchette and could also be important marker for spermatid‐specific functions.…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of apelin receptor (APJ) in mouse seminiferous epithelium

Das,

Gurusubramanian,

Roy

2024

J Exp Zool Pt A

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The apelin receptor (APJ) belongs to the member of the G protein‐coupled receptor family, and expression of APJ has been reported in the different cell types of testis. The seminiferous tubules in the testis can be identified as different stages (I–XII). It has been also suggested that different factors could be expressed in stage and cell‐specific manner in the seminiferous tubules. Recently, we also shown that expression of APJ is developmentally regulated in the testis from PND1 to PND42. Therefore, we analyzed the expression of APJ in the testis of adult mice by immunohistochemistry. Immunohistochemistry showed that the APJ was highly specific for the round and elongated spermatids with stage‐dependent changes. The seminiferous tubules at stages I–VII showed APJ immunostaining in the spermatid steps 1–8, not steps of 13–16. The seminiferous tubules at stages IX–XII showed APJ immunostaining in the spermatid steps 9–12. These results suggested the possible role of APJ in the spermiogenesis process. The intratesticular administration of APJ antagonist, ML221 showed a few round spermatids in the seminiferous tubules and some of the tubules with complete absence of round spermatid. Overall, we present evidence that APJ expression in spermatid is dependent on the stages of the seminiferous epithelium cycle and APJ could be involved in the differentiation of round spermatid to elongated spermatid.

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“…It has been reported that slowed basal metabolism in hypothyroidism causes changes in reactive oxygen and nitrogen species and antioxidant defense system in humans and different living species (Venditti and Di Meo, 2006). In several previous studies, it has been emphasized that changes in thyroid hormone levels have significant effects on different organs (Faraji et al, 2016;Monnereau et al, 2013;Obradovic et al, 2016) including liver, heart, skeletal muscles and brain and can induce oxidative stress (Baghcheghi et al, 2017;Beheshti et al, 2017). On the other hand, it was reported that in case of weakening of the antioxidant defense system, oxidative stress may increase thus oxidative damage may occur in lipid, protein and DNA (Kalyanaraman, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effect of levothyroxine administration on oxidative stress and cytokine levels in rats with experimental hypothyroidism

Bozbek,

Şentürk

2023

J Hellenic Vet Med Soc

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In this study, it was aimed to determine the effects of levothyroxine (L-T4) administration on serum free triiodothyronine (fT3), free thyroxine (fT4), thyroid stimulating hormone (TSH) and serum total antioxidant capacity (TAC), serum total oxidant capacity (TOC), oxidative stress index (OSI) and superoxide dismutase (SOD) activity, liver malondialdehyde (MDA) and the effects of cytokines on tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels in rats with experimental hypothyroidism. In the study, 48, 39-day-old, male Wistar albino rats were randomly divided into 3 groups containing 16 rats in each as control, hypothyroid and levothyroxine (L-T4) treated hypothyroid group. The rats in the control group were injected with 1 mL of saline and the rats in the hypothyroid group were injected via intraperitoneal (i.p.) route with 6-n-propyl thiouracil (PTU) at a dose of 10 mg/kg/day for 28 days. In the levothyroxine treated group, after induction of hypothyroidism 5 µg of levothyroxine diluted in 1 mL of sodium chloride (NaCl) administered via i.p. route for 15 days. At the end of the study, serum and liver samples were taken from the rats. In hypothyroidism group, serum fT3, fT4 and TAK levels as well as SOD (P<0.001) activity decreased statistically compared to the control group while serum TSH, TOC, OSI (P<0.001), liver MDA (P<0.01), TNF-α and IL-6 (P<0.001) levels increased significantly. The decreased serum fT3, fT4, TAC levels and SOD (P<0.001) activity in the hypothyroidism group increased with levothyroxine administration whereas the increased serum TSH, TOC, OSI and tissue MDA (P<0.01), TNF-α and IL-6 (P<0.001) levels were found to be decreased and approached to control levels. In conclusion, it has been shown that levothyroxine administration is effective against PTU-induced hypothyroidism in rats, inhibits lipid peroxidation and increases antioxidant status, minimizes the levels of inflammatory markers and protects against PTU-induced liver damage in rats. Because, the potential therapeutic use of levothyroxine in the treatment of hypothyroidism is thought to be appropriate.

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Exogenous apelin changes alpha and beta myosin heavy chain mRNA expression and improves cardiac function in PTU-induced hypothyroid rats

Cited by 5 publications

References 28 publications

Moderate exercise mitigates cardiac dysfunction and injury induced by cyclosporine A through activation of the PGI2 / PPAR-γ signaling pathway

Moderate exercise mitigates cardiac dysfunction and injury induced by cyclosporine A through activation of the PGI2 / PPAR-γ signaling pathway

Immunolocalization of apelin receptor (APJ) in mouse seminiferous epithelium

Effect of levothyroxine administration on oxidative stress and cytokine levels in rats with experimental hypothyroidism

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