Exogenous application of salicylic acid enhanced the rutin accumulation and influenced the expression patterns of rutin biosynthesis related genes in Fagopyrum tartaricum Gaertn leaves

Sun, Zhaoxia; Hou, Siyu; Yang, Wude; Han, Yuanhuai

doi:10.1007/s10725-012-9688-0

Cited by 27 publications

(28 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…dtu.dk/services/TMHMM/#opennewwindow). The two OcFLS proteins showed highly similarity to previously reported FLSs from other plants, such as Cpur FLS1(BBA27023.1) and CpurFLS2(BBA024.1) from Cyclamen purpurascens [22], AcFLS (AFA55179.1) from Acacia confuse [33], FtFLS1(AEC33116.1) from Fagopyrum tataricum [34], as well as AtFLS1(NP_196481.1) and AtFLS3(NP_201164.1) from Arabidopsis thaliana [35]. The multiple sequence alignment indicated that both of the two proteins possessed the highly conserved ferrous iron binding motif HXDX 53 H (in these motifs X represents any amino acid) and the 2-oxoglutarate binding motif RXS, indicating that both belonged to 2-ODDs (Additional file 3: Figure S3).…”

Section: Cdna Sequence Isolation and Analysessupporting

confidence: 63%

Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq

Sun

Kong

2019

BMC Plant Biol

View full text Add to dashboard Cite

Background: Flavonol synthase (FLS) is the key enzyme responsible for the biosynthesis of flavonols, the most abundant flavonoids, which have diverse pharmaceutical effects. Flavonol synthase has been previously found in other species, but not yet in Ornithogalum caudatum. Results: The transcriptome-wide mining and functional characterisation of a flavonol synthase gene family from O. caudatum were reported. Specifically, a small FLS gene family harbouring two members, OcFLS1 and OcFLS2, was isolated from O. caudatum based on transcriptome-wide mining. Phylogenetic analysis suggested that the two proteins showed the closest relationship with FLS proteins. In vitro enzymatic assays indicated OcFLS1 and OcFLS2 were flavonol synthases, catalysing the conversion of dihydroflavonols to flavonols in an iron-dependent fashion. In addition, the two proteins were found to display flavanone 3β-hydroxylase (F3H) activity, hydroxylating flavanones to form dihydroflavonols. Unlike single F3H enzymes, the F3H activity of OcFLS1 and OcFLS2 did not absolutely require iron. However, the presence of sufficient Fe 2+ was demonstrated to be conducive to successive catalysis of flavanones to flavonols. The qRT-PCR analysis demonstrated that both genes were expressed in the leaves, bulbs, and flowers, with particularly high expression in the leaves. Moreover, their expression was regulated by developmental and environmental conditions. Conclusions: OcFLS1 and OcFLS2 from O. caudatum were demonstrated to be flavonol synthases with ironindependent flavanone 3-hydroxylase activity.

show abstract

Section: Cdna Sequence Isolation and Analysessupporting

confidence: 63%

Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq

Sun

Kong

2019

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…SA treatment enhances rutin accumulation in India buckwheat ( Fagopyrum tartaricum) [ 42 ] and MeJA treatment induces myricetin, quercetin, and kaempferol in red raspberries ( Rubus idaeus L.) [ 43 ] and kale ( Brassica oleracea L.) leaves [ 44 ]. Consistent with our current study, chlorogenic acid was lower in MeJA-treated lettuce than in the control [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Plant-growth regulators alter phytochemical constituents and pharmaceutical quality in Sweet potato (Ipomoea batatas L.)

Ghasemzadeh

Talei

Jaafar

et al. 2016

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundSweet potato (Ipomoea batatas L.) is one of the most important consumed crops in many parts of the world because of its economic importance and content of health-promoting phytochemicals.MethodsWith the sweet potato (Ipomoea batatas L.) as our model, we investigated the exogenous effects of three plant-growth regulators methyl jasmonate (MeJA), salicylic acid (SA), and abscisic acid (ABA) on major phytochemicals in relation to phenylalanine ammonia lyase (PAL) activity. Specifically, we investigated the total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and total β-carotene content (TCC). Individual phenolic and flavonoid compounds were identified using ultra-high performance liquid chromatography (UHPLC). Antioxidant activities of treated plants were evaluated using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and a β-carotene bleaching assay. Anticancer activity of extracts was evaluated against breast cancer cell lines (MCF-7 and MDA-MB-231) using MTT assay.ResultsTPC, TFC, TAC, and TCC and antioxidant activities were substantially increased in MeJA-, SA-, and ABA-treated plants. Among the secondary metabolites identified in this study, MeJA application significantly induced production of quercetin, kaempferol, myricetin, gallic acid, chlorogenic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid. Luteolin synthesis was significantly induced by SA application. Compared with control plants, MeJA-treated sweet potato exhibited the highest PAL activity, followed by SA and ABA treatment. The high DPPH activity was observed in MeJA followed by SA and ABA, with half-maximal inhibitory concentration (IC50) values of 2.40, 3.0, and 3.40 mg/mL compared with α-tocopherol (1.1 mg/mL). Additionally, MeJA-treated sweet potato showed the highest β-carotene bleaching activity, with an IC50 value of 2.90 mg/mL, followed by SA (3.30 mg/mL), ABA (3.70 mg/mL), and control plants (4.5 mg/mL). Extracts of sweet potato root treated with MeJA exhibited potent anticancer activity with IC50 of 0.66 and 0.62 mg/mL against MDA-MB-231 and MCF-7 cell lines respectively, compared to that of extracts of sweet potato treated with SA (MDA-MB-231 = 0.78 mg/mL; MCF-7 = 0.90 mg/mL) and ABA (MDA-MB-231 = 0.94 mg/mL; MCF-7 = 1.40 mg/mL). The results of correlation analysis showed that anthocyanins and flavooids are corresponding compounds in sweet potato root extracts for anticancer activity against breast cancer cell lines.ConclusionsMeJA has great potential to enhance the production of important health-promoting phytochemicals in sweet potato.

show abstract

“…We also found that rutin was higher in the SA (227.39 g/g) and ETH (210.40 g/g) treated plants, compared to the control plants (177.33 g/g), but was lower in the JA (165.72 g/g) and ABA (137.36 g/g) treated plants. A previous study showed that rutin content significantly increased after SA treatment in Fagopyrum tartaricum leaves (Sun et al, 2012b), and Dong et al (2010) demonstrated that SA enhanced the levels of phenolic compounds, and elevated the activities of secondary metabolites and antioxidative enzymes in Salvia miltiorrhiza cell cultures. The effect of ABA increased the flavonol concentrations, especially quercetin and kampherol in grapes (Sandhu et al, 2011;Berli et al, 2011).…”

Section: Effect Of Phytohormone Treatments On Individual Phenolic Commentioning

confidence: 93%

Exogenous phytohormones increase the accumulation of health-promoting metabolites, and influence the expression patterns of biosynthesis related genes and biological activity in Chinese cabbage (Brassica rapa spp. pekinensis)

Thiruvengadam

Kim

Chung

2015

Scientia Horticulturae

View full text Add to dashboard Cite

Exogenous application of salicylic acid enhanced the rutin accumulation and influenced the expression patterns of rutin biosynthesis related genes in Fagopyrum tartaricum Gaertn leaves

Cited by 27 publications

References 17 publications

Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq

Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq

Plant-growth regulators alter phytochemical constituents and pharmaceutical quality in Sweet potato (Ipomoea batatas L.)

Exogenous phytohormones increase the accumulation of health-promoting metabolites, and influence the expression patterns of biosynthesis related genes and biological activity in Chinese cabbage (Brassica rapa spp. pekinensis)

Contact Info

Product

Resources

About