Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress

Koç, Esra

doi:10.17221/86/2014-pps

Cited by 6 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Higher Spd content will contribute to its greater availability for catabolism, conjugation, and other regulatory roles. The mode of action of free Spd and Spm in plant resistance against fungal pathogens has been described through their interactions with PAOs, antioxidant systems, and defense-signaling pathways as reported in several studies (Koç, 2015; Takahashi, 2016; Pal and Janda, 2017; Seifi and Shelp, 2019). Higher Spd/Put ratio in the host plant could also potentially affect uptake of PAs by the fungus especially, Put uptake by A. flavus from the maize kernels during pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Contribution of Maize Polyamine and Amino Acid Metabolism Toward Resistance Against Aspergillus flavus Infection and Aflatoxin Production

et al. 2019

View full text Add to dashboard Cite

Polyamines (PAs) are ubiquitous polycations found in plants and other organisms that are essential for growth, development, and resistance against abiotic and biotic stresses. The role of PAs in plant disease resistance depends on the relative abundance of higher PAs [spermidine (Spd), spermine (Spm)] vs. the diamine putrescine (Put) and PA catabolism. With respect to the pathogen, PAs are required to achieve successful pathogenesis of the host. Maize is an important food and feed crop, which is highly susceptible to Aspergillus flavus infection. Upon infection, the fungus produces carcinogenic aflatoxins and numerous other toxic secondary metabolites that adversely affect human health and crop value worldwide. To evaluate the role of PAs in aflatoxin resistance in maize, in vitro kernel infection assays were performed using maize lines that are susceptible (SC212) or resistant (TZAR102, MI82) to aflatoxin production. Results indicated significant induction of both PA biosynthetic and catabolic genes upon A. flavus infection. As compared to the susceptible line, the resistant maize lines showed higher basal expression of PA metabolism genes in mock-inoculated kernels that increased upon fungal infection. In general, increased biosynthesis and conversion of Put to Spd and Spm along with their increased catabolism was evident in the resistant lines vs. the susceptible line SC212. There were higher concentrations of amino acids such as glutamate (Glu), glutamine (Gln) and γ-aminobutyric acid (GABA) in SC212. The resistant lines were significantly lower in fungal load and aflatoxin production as compared to the susceptible line. The data presented here demonstrate an important role of PA metabolism in the resistance of maize to A. flavus colonization and aflatoxin contamination. These results provide future direction for the manipulation of PA metabolism in susceptible maize genotypes to improve aflatoxin resistance and overall stress tolerance.

show abstract

Section: Discussionmentioning

confidence: 99%

Contribution of Maize Polyamine and Amino Acid Metabolism Toward Resistance Against Aspergillus flavus Infection and Aflatoxin Production

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Exogenous PA application enhanced plant tolerance/resistance to several abiotic stress conditions, such as salinity, drought, water logging/flooding, osmotic stress, heavy metals, and extreme temperatures (Liu et al., 2006, 2015; Moschou et al., 2008c, 2012; Paschalidis et al., 2009a; Toumi et al., 2010; Moschou and Roubelakis-Angelakis, 2014; Gupta et al., 2016; Handa et al., 2018; Nguyen et al., 2018; Tiburcio and Alcazar, 2018; Pal et al., 2019; Yin et al., 2019). Polyamine application also enhanced tolerance to a few phytopathogenic infections in planta , such as Alternaria alternata (Estiarte et al., 2017), Phytophthora capsici (Koç, 2015), and Pseudomonas viridiflava (Rossi et al., 2015, 2018), and in vitro , such as Fusarium strains (Wojtasik et al., 2015) and Sclerotinia sclerotiorum (Garriz et al., 2003). The increase of host PA levels, either by using transgenic method or treatment with exogenous PAs, strongly decreased in planta growth of biotrophic pathogen Pseudomonas viridiflava , which was relieved by a PA oxidase (PAO) inhibitor (Marina et al., 2008).…”

Section: Introductionmentioning

confidence: 99%

Polyamine Catabolism in Plants: A Universal Process With Diverse Functions

et al. 2019

View full text Add to dashboard Cite

Polyamine (PA) catabolic processes are performed by copper-containing amine oxidases (CuAOs) and flavin-containing PA oxidases (PAOs). So far, several CuAOs and PAOs have been identified in many plant species. These enzymes exhibit different subcellular localization, substrate specificity, and functional diversity. Since PAs are involved in numerous physiological processes, considerable efforts have been made to explore the functions of plant CuAOs and PAOs during the recent decades. The stress signal transduction pathways usually lead to increase of the intracellular PA levels, which are apoplastically secreted and oxidized by CuAOs and PAOs, with parallel production of hydrogen peroxide (H 2 O 2 ). Depending on the levels of the generated H 2 O 2 , high or low, respectively, either programmed cell death (PCD) occurs or H 2 O 2 is efficiently scavenged by enzymatic/nonenzymatic antioxidant factors that help plants coping with abiotic stress, recruiting different defense mechanisms, as compared to biotic stress. Amine and PA oxidases act further as PA back-converters in peroxisomes, also generating H 2 O 2 , possibly by activating Ca 2+ permeable channels. Here, the new research data are discussed on the interconnection of PA catabolism with the derived H 2 O 2 , together with their signaling roles in developmental processes, such as fruit ripening, senescence, and biotic/abiotic stress reactions, in an effort to elucidate the mechanisms involved in crop adaptation/survival to adverse environmental conditions and to pathogenic infections.

show abstract

“…It is well acknowledged that polyamines contribute to plant resistance against fungal pathogens by multiple functions (KoÇ 2015;Takahashi 2016;Gonzalez et al 2021). Polyamines have a crucial impact on regulating the production of hydrogen peroxide (H 2 O 2 ), which is recognized as the primary reactive oxygen species (ROS) in plants.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of wheat spermidine synthase gene enhances wheat resistance to Fusarium head blight

Ren,

Li,

Xiu

et al. 2024

Phytopathol Res

View full text Add to dashboard Cite

Polyamines, such as putrescine, spermidine, and spermine, are crucial for plant defense against both abiotic and biotic stresses. Putrescine is also known as a significant inducer of deoxynivalenol (DON) production in Fusarium graminearum, the primary causal agent of Fusarium head blight (FHB). However, the impact of other polyamines on DON production and whether modifying polyamine biosynthesis could improve wheat resistance to FHB are currently unknown. In this study, we demonstrate that key precursor components of putrescine synthesis, including arginine, ornithine, and agmatine, can induce DON production, albeit to a lesser extent than putrescine in trichothecene biosynthesis-inducing (TBI) culture under the same total nitrogen conditions. Intriguingly, spermidine and spermine, downstream products of putrescine in the polyamine biosynthesis pathway, do not induce DON production under the same conditions. Additionally, externally applying either spermidine or spermine to wheat heads significantly reduces the diseased spikelet number caused by F. graminearum. Furthermore, our results show that overexpression of the wheat spermidine synthase (SPDS) gene TaSPDS-7D1 significantly enhances the spermidine content and wheat resistance to FHB. In addition, the TaSPDS-7D1-overexpressing line OE3 exhibited a 1000-grain weight and plant height increase compared to the wild type. Our findings reveal that overexpression of the spermidine synthase gene can enhance wheat resistance to FHB without compromising wheat yield.

show abstract

Exogenous application of spermidine enhanced tolerance of pepper against Phytophthora capsici stress

Cited by 6 publications

References 29 publications

Contribution of Maize Polyamine and Amino Acid Metabolism Toward Resistance Against Aspergillus flavus Infection and Aflatoxin Production

Contribution of Maize Polyamine and Amino Acid Metabolism Toward Resistance Against Aspergillus flavus Infection and Aflatoxin Production

Polyamine Catabolism in Plants: A Universal Process With Diverse Functions

Overexpression of wheat spermidine synthase gene enhances wheat resistance to Fusarium head blight

Contact Info

Product

Resources

About