Exogenous caffeic acid and epicatechin enhance resistance against Botrytis cinerea through activation of the phenylpropanoid pathway in apples

Zhang, Mengyu; Wang, Dajiang; Gao, Xixi; Yue, Zhengyang; Zhou, Huiling

doi:10.1016/j.scienta.2020.109348

Cited by 80 publications

(21 citation statements)

References 33 publications

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“…In this report, exogenous caffeic acid markedly increased PAL and POD enzymatic activities, which were significantly higher than those in the control group ( Figures 6A,B ). Similarly, Zhang et al (2020) found that caffeic acid treatment increased POD and PAL enzyme activity in apples and significantly inhibited Botrytis cinerea infection. Additionally, lignin and hydroxyproline are major secondary products in plants with important disease resistance functions.…”

Section: Discussionmentioning

confidence: 85%

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Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum

Jing

Zhu

et al. 2021

Front. Plant Sci.

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In rhizospheres, chemical barrier-forming natural compounds play a key role in preventing pathogenic bacteria from infecting plant roots. Here, we sought to identify specific phenolic exudates in tobacco (Nicotiana tobaccum) plants infected by the soil-borne pathogen Ralstonia solanacearum that may exhibit antibacterial activity and promote plant resistance against pathogens. Among detected phenolic acids, only caffeic acid was significantly induced in infected plants by R. solanacearum relative to healthy plants, and the concentration of caffeic acid reached 1.95 μg/mL. In vivo, caffeic acid at 200 μg/mL was highly active against R. solanacearum and obviously damaged the membrane structure of the R. solanacearum cells, resulting in the thinning of the cell membrane and irregular cavities in cells. Moreover, caffeic acid significantly inhibited biofilm formation by repressing the expression of the lecM and epsE genes. In vitro, caffeic acid could effectively activate phenylalanine ammonia-lyase (PAL) and peroxidase (POD) and promote the accumulation of lignin and hydroxyproline. In pot and field experiments, exogenous applications of caffeic acid significantly reduced and delayed the incidence of tobacco bacterial wilt. Taken together, all these results suggest that caffeic acid played a crucial role in defending against R. solanacearum infection and was a potential and effective antibacterial agent for controlling bacterial wilt.

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Section: Discussionmentioning

confidence: 85%

“…The roots and stems of each plant were rapidly frozen in liquid nitrogen, ground into powder, and stored at −80°C for subsequent determination. The activity of phenylalanine ammonia-lyase (PAL) and peroxidase (POD) was assayed according to Zhang et al (2020) . The PAL and POD activities were represented as U min –1 g –1 FW.…”

Section: Methodsmentioning

confidence: 99%

Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum

Jing

Zhu

et al. 2021

Front. Plant Sci.

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“…Consistent with this result, the structural genes involved in the synthesis of caffeic acid ( HCT and CSE ) were significantly up-regulated in the inoculated treatment ( Figure 3 ; Supplementary Figures 12 , 16 ). In apple, caffeic acid has been shown to effectively promote lignin accumulation and inhibit gray mold infection (Zhang et al, 2020 ). Although the accumulation of lignin was not detected in our study, we detected significant accumulation of the precursors precursor of G-lignin (coniferyl alcohol) and S-lignin (sinapic acid and sinapinaldehyde) ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolic and Transcriptomic Profiling of Lilium Leaves Infected With Botrytis elliptica Reveals Different Stages of Plant Defense Mechanisms

Chai

Zuo

et al. 2021

Front. Plant Sci.

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Botrytis elliptica, the causal agent of gray mold disease, poses a major threat to commercial Lilium production, limiting its ornamental value and yield. The molecular and metabolic regulation mechanisms of Lilium's defense response to B. elliptica infection have not been completely elucidated. Here, we performed transcriptomic and metabolomic analyses of B. elliptica resistant Lilium oriental hybrid “Sorbonne” to understand the molecular basis of gray mold disease resistance in gray mold disease. A total of 115 differentially accumulated metabolites (DAMs) were detected by comparing the different temporal stages of pathogen infection. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed the differentially expressed genes (DEGs) and DAMs were enriched in the phenylpropanoid and flavonoid pathways at all stages of infection, demonstrating the prominence of these pathways in the defense response of “Sorbonne” to B. elliptica. Network analysis revealed high interconnectivity of the induced defense response. Furthermore, time-course analysis of the transcriptome and a weighted gene coexpression network analysis (WGCNA) led to the identification of a number of hub genes at different stages, revealing that jasmonic acid (JA), salicylic acid (SA), brassinolide (BR), and calcium ions (Ca2+) play a crucial role in the response of “Sorbonne” to fungal infection. Our work provides a comprehensive perspective on the defense response of Lilium to B. elliptica infection, along with a potential transcriptional regulatory network underlying the defense response, thereby offering gene candidates for resistance breeding and metabolic engineering of Lilium.

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“…Increased Na + activity disorganize ionic balance in cells and damages plasma membrane with illegitimate production of photosynthetic pigments [ 45 ]. Caffeic acid application significantly improved the plant water contents by improving the surface area and capacity of plant roots to absorb more water from the rhizosphere, balanced osmotic regulation, transpiration, stomatal conductance and increase plant water potential [ 46 ]. Moreover, caffeic acid regulates antioxidant defense by acting as a scavenger against H 2 O 2 and O 2 - and minimize ROS production at the cellular level which increases membrane stability [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Assessing the potential of exogenous caffeic acid application in boosting wheat (Triticum aestivum L.) crop productivity under salt stress

et al. 2021

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Caffeic acid (CA) is known as an antioxidant to scavenge reactive oxygen species (ROS), but the underlying mechanism of mediation of plant salt tolerance against various abiotic stresses by caffeic acid is only partially understood. A field experiment (120 days duration) was conducted to investigate the protective role of caffeic acid under a high saline medium (EC 8.7 dS m-1 and textural class: sandy loam) in two wheat genotypes (FSD -08 and Zincol-16). Two levels of caffeic acid (50 μM and 100 μM) were applied exogenously in combination with the salinity stress and results revealed that salt alleviation is more prominent when caffeic acid was applied at the rate of 100 μM. Under saline conditions, wheat genotypes show poor fresh and dry matter accumulation, chlorophyll contents, relative water contents (RWC), membrane stability index (MSI) and activities of antioxidant enzymes and increased uptake of Na+ ions. However, wheat genotype FSD-08 eminently responded to caffeic acid application as compared to wheat genotype Zincol-16 as demonstrated by higher growth indicators, RWC, MSI, activities of antioxidant enzymes, accumulation of mineral ions in grain along with yield attributes. In addition, caffeic acid also mitigated salt-induced oxidative stress malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents as well as significantly reduced Na+ uptake. It can be concluded that caffeic acid-induced salinity tolerance in wheat is attributed to improved plant water relations, K+ uptake, yield contents and activities of antioxidant stress enzymes.

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Exogenous caffeic acid and epicatechin enhance resistance against Botrytis cinerea through activation of the phenylpropanoid pathway in apples

Cited by 80 publications

References 33 publications

Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum

Caffeic Acid in Tobacco Root Exudate Defends Tobacco Plants From Infection by Ralstonia solanacearum

Metabolic and Transcriptomic Profiling of Lilium Leaves Infected With Botrytis elliptica Reveals Different Stages of Plant Defense Mechanisms

Assessing the potential of exogenous caffeic acid application in boosting wheat (Triticum aestivum L.) crop productivity under salt stress

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