Exogenous calcium chloride (CaCl2) promotes γ-aminobutyric acid (GABA) accumulation in fresh-cut pears

Chi, Zongyu; Dai, Yuqin; Cao, Shifeng; Wei, Yingying; Shao, Xingfeng; Huang, Xiaosan; Xu, Feng; Wang, Hongfei

doi:10.1016/j.postharvbio.2020.111446

Cited by 25 publications

(15 citation statements)

References 37 publications

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“…Exogenous calcium chloride (CaCl 2 ) could promote GABA accumulation in shredded carrots [ 9 ]. Similarly, CaCl 2 activated GABA shunt through extracellular Ca 2+ influx, which enhanced the GABA production of fresh-cut pears [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Calcium Involved in the Enrichment of γ-Aminobutyric Acid (GABA) in Broccoli Sprouts under Fructose Treatment

Wei

Xie

Wang

et al. 2023

Plants

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The effect of fructose on γ-aminobutyric acid (GABA) content and its metabolic pathway in broccoli sprouts was investigated. The results demonstrated that the fructose treatment not only significantly increased the fresh weight, GABA, and glutamate contents in sprouts, but also promoted the activity of glutamic acid decarboxylase (GAD) and the expressions of BoGAD1 and BoGAD2. Meanwhile, fructose treatment inhibited the stem length of broccoli sprouts and enhanced the abscisic acid (ABA) production in comparison with the control. Ca2+, CaM contents, and BoCaM2 expression in broccoli sprouts were also stimulated after fructose treatment. Exogenous fructose increased inositol trisphosphate (IP3) content and activated the activity of phosphatidylinositol-specific phospholipase C (PI-PLC) and the expression of BoPLC2, contributing to Ca2+ influx into the cells. These results suggested that Ca2+ played an essential role in GABA enrichment under fructose treatment, which may be associated with GAD and PI-PLC.

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Section: Introductionmentioning

confidence: 99%

Calcium Involved in the Enrichment of γ-Aminobutyric Acid (GABA) in Broccoli Sprouts under Fructose Treatment

Wei

Xie

Wang

et al. 2023

Plants

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“…GABA in higher plants is mainly produced from the glutamate, catalyzed by glutamate decarboxylase (GAD) (Chi et al, 2021;Shelp et al, 1999), some of which also comes from polyamines degradation (Kim et al, 2013;Liao et al, 2017). The pathway of putrescine-derived GABA formation involves a two-step reaction, including diamine oxidase (DAO), or amine oxidase (CuAO), or polyamine oxidase (PAO) catalytic process and followed by an aminoaldehyde dehydrogenase (AMADH) degradation (Moschou et al, 2012;Zhang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Although there is only preliminary study of biochemical properties, AMADHs has become a hot topic in physiological processes (Arikit et al, 2011). Some other studies also reported the accumulation of GABA in horticultural products, regulated by the function of AMADH (Wang et al, 2019;Chi et al, 2021). Previous studies have shown that GABA is involved in a variety of abiotic and biotic stress responses, while very little is known about the involvement of CuAO1 together with AMADH1 responsive to drought stress.…”

Section: Introductionmentioning

confidence: 99%

CsCuAO1 associated with CsAMADH1 confers drought tolerance by modulating GABA levels in the tea plant

Zhu

Cao

Zhang

et al. 2023

Preprint

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Our previous study has shown that copper-containing amine oxidase ( CuAO) and aminoaldehyde dehydrogenase ( AMADH) could regulate the accumulation of γ-aminobutyric acid (GABA) in tea plants by participating in polyamine degradation pathway. However, the involvement of these genes in drought tolerance has not been underlined. In this study, CsCuAO1 associated with CsAMADH1 confers drought tolerance, which modulates GABA levels in tea plants, was conducted. The results showed that exogenous GABA spraying effectively alleviated the drought induced physical damage. Overexpression Arabidopsis lines of CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought, which promoted the synthesis of GABA and putrescine, by alerting reactive oxygen species scavenging capacity and stomatal movement. However, suppression of CsCuAO1 or CsAMADH1 in tea plants exhibited increased sensitivity during drought treatment. Moreover, the co-overexpressed plants increased the accumulation of GABA both in the Agrobacterium-mediated Nicotiana benthamiana transient assay and transgenic Arabidopsis plants. In addition, a GABA transporter, CsGAT1, was identified, whose expression is strongly correlated with GABA accumulation levels in different tissues under drought stress. Taken together, CsCuAO1 and CsAMADH1 were involved in response to drought stress through a dynamic balance between GABA and putrescine. Our data will greatly contribute to the characterization of GABA biological functions in response to environmental stresses in plants.

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“…In polyamine degradation pathway, the oxidation of putrescine (Put), spermidine (Spd) and spermine (Spm) contributes directly to GABA accumulation [ 16 ]. The enzymes involved in the oxidative deamination of PAs are diamine oxidases (DAO) and polyamine oxidase (PAO), both of them catalyze Put, Spd and Spm to 4-aminobutyraldehyde (ABAL) and aminoaldehyde dehydrogenase (AMADH) thereafter to produce GABA [ 17 , 18 ]. Information about GABA accumulation in watercore fruit is limited.…”

Section: Introductionmentioning

confidence: 99%

Watercore Pear Fruit Respiration Changed and Accumulated γ-Aminobutyric Acid (GABA) in Response to Inner Hypoxia Stress

Liu

Chen

et al. 2022

Genes

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Watercore is a physiological disorder which often occurs on the pear fruit and the excessive accumulation of sorbitol in fruit intercellular space is considered to be an important cause of watercore. Our previous studies found that the metabolic disorder of sugars may lead to hypoxia stress and disturb respiration, resulting in aggravated fruit rot and the formation of bitter substances. However, the further changes of respiration and the fruit response mechanism are not well understood. A comprehensive transcriptome analysis of ‘Akibae’ pear watercore fruit was performed in this study. The transcriptome results revealed the hypoxia stress significantly induced the expression of several key enzymes in the TCA cycle and may lead to the accumulation of succinic acid in watercore fruit. The glycolytic pathway was also significantly enhanced in watercore fruit. Moreover, the γ-aminobutyric acid (GABA) synthesis related genes, glutamate decarboxylase (GAD) genes and polyamine oxidase (PAO) genes, which associated with the GABA shunt and the polyamine degradation pathway were significantly upregulated. In addition, the PpGAD1 transcript level increased significantly along with the increase of GAD activity and GABA content in the watercore fruit. Above all, these findings suggested that the hypoxic response was marked by a significant increase of the hypoxia-inducible metabolites succinic acid and GABA and that PpGAD1 may play a key role in response to watercore by controlling the GABA synthesis.

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Exogenous calcium chloride (CaCl2) promotes γ-aminobutyric acid (GABA) accumulation in fresh-cut pears

Cited by 25 publications

References 37 publications

Calcium Involved in the Enrichment of γ-Aminobutyric Acid (GABA) in Broccoli Sprouts under Fructose Treatment

Calcium Involved in the Enrichment of γ-Aminobutyric Acid (GABA) in Broccoli Sprouts under Fructose Treatment

CsCuAO1 associated with CsAMADH1 confers drought tolerance by modulating GABA levels in the tea plant

Watercore Pear Fruit Respiration Changed and Accumulated γ-Aminobutyric Acid (GABA) in Response to Inner Hypoxia Stress

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