Exogenous endothelial cells as accelerators of hematopoietic reconstitution

Mizer, J Christopher; Ichim, Thomas E.; Alexandrescu, Doru T; Dasanu, Constantin A; Ramos, Famela; Turner, A. Robert; Woods, Erik J.; Bogin, Vladimir; Murphy, Michael P.; Koos, David; Patel, Amit N.

doi:10.1186/1479-5876-10-231

Cited by 5 publications

(3 citation statements)

References 123 publications

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“…Presently, the most feasible source for obtaining sufficient amount of endothelial cells for clinical use or expansion of HSCs with relative ease is the stromal vascular fraction from fat tissues; although currently, few investigators have explored this option. Stromal Vascular Fraction (SVF) is a heterogenous cellular product comprised of the mononuclear cells derived from adipose tissue and it is known to contain not only endothelial cells, but also T regulatory cells, monocytes, mesenchymal progenitor/stem cells, preadipocytes, pericytes and even hematopoietic stem cells plus a variety of other cells [7]. Essentially, SVF is adipose tissue deprived of the mature adipocytes.…”

Section: Endogenous Sources Of Endothelial Cellsmentioning

confidence: 99%

A novel way of harvesting a large population of endothelial cells for clinical and experimental use

Ilesanmi¹

2018

Biomed Res Rev

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For in vitro cultural purposes, cytokines and a variety of other synthetic constituents have been used in time past to support the growth of Hematopoietic Stem Cells (HSCs) and many other critical human cells. However, in the case of HSCs, it has not been possible to ensure their long-term survival and expansion by merely using cocktails of these cytokines. Endothelial Cells (EC), the main type of cells lining the blood vessels, lymphatic vessels and the heart are favorable to HSC growth. When in co-culture with HSCs, ECs have been found to be key in supporting HSCs in vitro. Harvesting sufficient number of endogenous ECs is critical for these cells' utility in experiments and for clinical purposes. The number of natural endogenous ECs obtainable by most current procedures fall short of that required for clinical application and HSC co-culture purposes; hence researchers have now resorted to the use of synthetic brand of ECs which have its own shortcomings. Although good for experimental purposes, it may not be easily adaptable for clinical therapy. This review is intended to blend the current technologies in use for therapy to formulate a procedure for obtaining large numbers of endothelial cells that could be used in co-culture with HSCs or even for use in clinical therapy.

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Section: Endogenous Sources Of Endothelial Cellsmentioning

confidence: 99%

A novel way of harvesting a large population of endothelial cells for clinical and experimental use

Ilesanmi¹

2018

Biomed Res Rev

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“…Endogenously-derived endothelial cells may be derived in large numbers by the protocol described earlier in a previous publication [17]. The EC cells are obtained from the same individual that is to be transplanted with the HSCs.…”

Section: Summarized Procedures For Obtaining Autologous Ecs For Co-culturementioning

confidence: 99%

Achieving boosted expansion of transplantable hematopoietic stem cells by co-culture with autologous endothelial cells

Ilesanmi¹

2018

Biomed Res Rev

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Currently, hematopoietic stem cells derived from the bone marrow or from cord blood are used to treat patients who require hematopoietic stem cell treatment. In spite of the varied sources from which hematopoietic stem cells may be obtained, there is still shortage of genetically matched hematopoietic stem cell grafts. As a result, many patients requiring transplantation cannot benefit from the procedure. Efforts have been made in time past to expand this unique type of adult stem cells in culture, with little success. These stem cells, even in the presence of several exogenous cytokines, serum factors and support from (mesenchymal) cells do not propagate the stem cells beyond a few days. However, reports from recent studies indicate that long-term propagation and large-scale expansion of hematopoietic stem cells is now possible with the use of endothelial cells in co-culture with hematopoietic stem cells. These studies have demonstrated that the presence of endothelial cells is necessary for promoting self-renewal of Hematopoietic Stem Cells (HSC), for long-term survival of repopulating HSCs and for increasing the number of these cells in culture. Endothelial cells from blood vessels constitute the basic building blocks of the human vasculature, being a key constituent of the bone marrow and vascular niche that support hematopoietic mobilization, maintenance and regeneration. Researchers have not only found that endothelial cells could greatly expand HSCs but the proliferation induced by endothelial cells carries no risk of tumor formation. There is a dire need to translate this revolutionary finding from the laboratory into routine clinical use. The purpose of this review therefore, is to glean the several studies related to the subject into a practical protocol scalable for clinical application.

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“…It has been reported that >10 types of cell growth factors exist with various stimulation effects. Cell growth factors are part of a complex system that has several influencing factors ( 3 – 5 ). The secretion, release and function of cell growth factors depend on the hematopoietic microenvironment, which is important for HSC growth and development.…”

Section: Introductionmentioning

confidence: 99%

Effects of methylthiouracil on the proliferation and apoptosis of rat bone marrow stromal cells

Hou

Chen

Ding

et al. 2014

Experimental and Therapeutic Medicine

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The aim of the present study was to investigate the effects of methylthiouracil (MTU) on the proliferation and apoptosis of rat bone marrow stromal cells (BMSCs). Rat BMSCs were isolated, cultured in vitro and treated with various concentrations of MTU. Cell growth curves were determined using the Cell Counting Kit-8 method and the effect of MTU on BMSCs in a logarithmic growth phase was observed. BMSC apoptosis following MTU treatment was detected by flow cytometry. The experimental results demonstrated that the proliferation-inhibition effect was gradually enhanced with increasing MTU concentrations and the extension of treatment time. Statistically significant differences were observed between the treatment and the control groups (P<0.05). In addition, the BMSC apoptosis rate gradually increased with increasing drug concentrations and treatment time extension; statistically significant differences were observed between the treatment and the control groups (P<0.05). Therefore, the results of the present study demonstrated that MTU inhibited the proliferation of BMSCs and promoted apoptosis, indicating the cytotoxic effects of MTU on BMSCs.

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Exogenous endothelial cells as accelerators of hematopoietic reconstitution

Cited by 5 publications

References 123 publications

A novel way of harvesting a large population of endothelial cells for clinical and experimental use

A novel way of harvesting a large population of endothelial cells for clinical and experimental use

Achieving boosted expansion of transplantable hematopoietic stem cells by co-culture with autologous endothelial cells

Effects of methylthiouracil on the proliferation and apoptosis of rat bone marrow stromal cells

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