Exogenous gibberellin weakens lipid breakdown by increasing soluble sugars levels in early germination of zanthoxylum seeds

Sun, Jia; Jia, Hao; Ping, Wang; Zhou, Tao; Wu, Yan; Liu, Zhiming

doi:10.1016/j.plantsci.2018.08.013

Cited by 25 publications

(18 citation statements)

References 26 publications

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“…Carbon and nitrogen metabolites, including those mentioned above, are significantly associated with germination and seedling establishment [13], during which GABA critically affects carbon and nitrogen metabolism [8]. During seed germination, as the hormone GA's role [53], exogenous GABA may promote starch hydrolysis to produce soluble sugars by stimulating α-amylase activity [20], but in the current study, there were no significant changes in starch contents following the GABA and VGB treatments ( Figure 3B). This may have resulted in the decrease in soluble sugar contents at 120 h ( Figure 3A), with the resulting lack of sufficient energy leading to the inhibition of early primary root growth (Figure 1).…”

Section: Discussionmentioning

confidence: 50%

“…This may have resulted in the decrease in soluble sugar contents at 120 h ( Figure 3A), with the resulting lack of sufficient energy leading to the inhibition of early primary root growth (Figure 1). However, we speculated that the increase in soluble sugar levels at 48 h may have resulted from the lipid breakdown occurring in germinating seeds [53]. We observed that both treatments induced the considerable accumulation of H 2 O 2 ( Figure 2B), which can be mainly produced by fatty acid β-oxidation during germination [53], contributing to the inhibition of chestnut seed germination.…”

Section: Discussionmentioning

confidence: 92%

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Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Chen

et al. 2020

Plants

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Recalcitrant chestnut seeds are rich in γ-aminobutyric acid (GABA), which negatively regulates adventitious root development by altering carbon/nitrogen metabolism. However, little is known regarding the role of this metabolite in chestnut seeds. In this study, we investigated the effects of GABA changes on the germination of chestnut seeds treated with exogenous GABA and vigabatrin (VGB, which inhibits GABA degradation). Both treatments significantly inhibited seed germination and primary root growth and resulted in the considerable accumulation of H2O2, but the endogenous GABA content decreased before germination at 48 h. Soluble sugar levels increased before germination, but subsequently decreased, whereas starch contents were relatively unchanged. Changes to organic acids were observed at 120 h after sowing, including a decrease and increase in citrate and malate levels, respectively. Similarly, soluble protein contents increased at 120 h, but the abundance of most free amino acids decreased at 48 h. Moreover, the total amino acid levels increased only in response to VGB at 0 h. Accordingly, GABA and VGB altered the balance of carbon and nitrogen metabolism, thereby inhibiting chestnut seed germination. These results suggested that changes to GABA levels in chestnut seeds might prevent seed germination. The study data may also help clarify the dormancy and storage of chestnut seeds, as well as other recalcitrant seeds.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 92%

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Chen

et al. 2020

Plants

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“…The balance of carbon and nitrogen in seeds affects seed development, maturation and germination statuses [6,[56][57]. During seed germination, the disturbance of endogenous γaminobutyric acid or activation of α-amylase activities contributes to starch hydrolysis [7,33,[57][58]. Carbohydrate degradation which accompanied by the accumulation of monosaccharides is expected to support germination [33].…”

Section: Discussionmentioning

confidence: 99%

Comparative Metabolome Analyses of Chinese Hickory Nut Kernels Highlight The Importance of Allocation Shift and Accumulation on Primary and Secondary Metabolites During In-Situ Germination

Xiao¹,

ong²,

Wang³

et al. 2020

Preprint

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Background: Chinese hickory ( Carya cathayensis ) and pecan ( C. illinoinensis ) are popular nut trees with high economic value in the family Juglandaceae, due to the richness in nutrients and multiple health factors in their nut kernels. However, the considerable in-situ germination of the nut seeds severely impact the quality of the kernels. The fundamental meta bolic mechanism of in-situ germination has been widely reported in major crops but rare in nut crops. Results: We sampled non- and pre-sprouted Chinese hickory nut kernels and performed liquid chromatography-tandem mass spectrometry (LC–MS/MS)-based metabolomic analyses. Comparative metabolomic analyses revealed 35 6 significantly-changed metabolites during in-situ germination. Detailed analysis emphasized the increasing accumulation and dynamic changes in compounds of primary mechanism and energy supply for in-situ germination. The results highlighted the importance of allocation shift and accumulation on four classes of primary metabolites and their derivatives, and two secondary metabolite classes between the transitions of germination initiation and germination and seedling establishment during the process. Metabolites related to carbohydrates and nitrogen metabolism, and few harmful to human health also demonstrated increasing accumulation along the process. Conclusions: The findings flourish our understanding of the metabolic mechanisms of in-situ germination and will be useful for future application in molecular breeding in Chinese hickory.

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“…A limited number of studies have shown that alterations in cell membrane lipids may be involved in the alleviation of seed dormancy in agriculture crop species [45,46,81]. For example, during germination seed membrane lipids were observed to be remodelled by the gradual increase of plastidic lipids, where the level of PA first decreased followed by an elevation (increase) before finally decreasing in soybeans [79][80][81][82].…”

Section: Role Of Lipid Metabolism In Resolving Seed Dormancy In Uplanmentioning

confidence: 99%

“…and biological (lipids, proteins, etc.) alterations [44][45][46][47][48]. Changes in seed membrane lipid metabolism have been reported to be highly correlated with improved seed germination [49].…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanoparticles Functionalized with Carboxylic Acid Improved the Germination and Seedling Vigor in Upland Boreal Forest Species

Ali

Sobze

et al. 2020

Nanomaterials

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Nanopriming has been shown to significantly improve seed germination and seedling vigor in several agricultural crops. However, this approach has not been applied to native upland boreal forest species with complex seed dormancy to improve propagation. Herein, we demonstrate the effectiveness of carbon nanoparticles functionalized with carboxylic acids in resolving seed dormancy and improved the propagation of two native upland boreal forest species. Seed priming with carbon nanoparticles functionalized with carboxylic acids followed by stratification were observed to be the most effective in improving germination to 90% in green alder (Alnus viridis L.) compared to 60% in the control. Conversely, a combination of carbon nanoparticles (CNPs), especially multiwall carbon nanoparticles functionalized with carboxylic acid (MWCNT–COOH), cold stratification, mechanical scarification and hormonal priming (gibberellic acid) was effective for buffaloberry seeds (Shepherdia canadensis L.). Both concentrations (20 µg and 40 µg) of MWCNT–COOH had a higher percent germination (90%) compared to all other treatments. Furthermore, we observed the improvement in germination, seedling vigor and resolution of both embryo and seed coat dormancy in upland boreal forest species appears to be associated with the remodeling of C18:3 enriched fatty acids in the following seed membrane lipid molecular species: PC18:1/18:3, PG16:1/18:3, PE18:3/18:2, and digalactosyldiacylglycerol (DGDG18:3/18:3). These findings suggest that nanopriming may be a useful approach to resolve seed dormancy issues and improve the seed germination in non-resource upland boreal forest species ideally suited for forest reclamation following resource mining.

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Exogenous gibberellin weakens lipid breakdown by increasing soluble sugars levels in early germination of zanthoxylum seeds

Cited by 25 publications

References 26 publications

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Comparative Metabolome Analyses of Chinese Hickory Nut Kernels Highlight The Importance of Allocation Shift and Accumulation on Primary and Secondary Metabolites During In-Situ Germination

Carbon Nanoparticles Functionalized with Carboxylic Acid Improved the Germination and Seedling Vigor in Upland Boreal Forest Species

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