Exogenous Hydrogen Sulfide Alleviates-Induced Intracellular Inflammation in HepG2 Cells

Abstract

Fatty acids induced hepatic inflammation plays an important role in nonalcoholic fatty liver disease (NAFLD) pathogenesis. Hydrogen sulfide (H2S), an endogenous gasotransmitter, has been established to possess potent anti-inflammation in various human organs. However, the anti-inflammation property of H2S in the fatty liver is still needed to further elucidate. Hence, this study aimed to investigate whether exogenous H2S can protect hepatocytes against inflammation induced by palmitic acid (PA). HepG2 hepatocytes were exposed to PA for 24 h to induce free fatty acids-induced inflammation. The cells were pretreated with NaHS (a donor of H2S) before exposure to PA. Cell viability, inflammatory cytokines (TNF-α, IL-6 and IL-1β), NLRP3 inflammasome and NF-κB were measured by a combination of MTT assay, ELISA, Western blot and Immunofluorescence. Here, we found that exogenous H2S dose-dependently inhibited the expression of pro-inflammatory cytokines, NLRP3 inflammasome and activation of NF-κB signaling in PA-induced HepG2 cells. Thus, H2S might be a candidate therapeutic agent against NAFLD.

Implications of hydrogen sulfide in liver pathophysiology: Mechanistic insights and therapeutic potential

“…Exogenous H 2 S donors are reported to protect HepG2 hepatocytes against palmitic acid-induced inflammation, implying that the H 2 S system may be a crucial target for the treatment of NAFLD through its anti-inflammatory effects [124] . The hepatic H 2 S levels, CBS and CSE expressions are impaired in rats with NASH induced by a methionine/choline-deficient diet, and treatment with exogenous H 2 S prevents the progression of NASH possibly through suppression of liver oxidative stress and inflammation [125] .…”

GYY4137 Ameliorates Sepsis-induced Cardiomyopathy via Inhibiting NLRP3 Inflammasome Activity and Reduction of Oxidative Stress in the Myocardium

“…Although H 2 S, a small molecule that is traditionally conceptualized as toxic, can exhibit neurotoxicity and reduced cell survival at higher concentrations [25], it was reported that in microglia (Microglial), high concentrations of H 2 S can increase cellular ROS [26]. The high concentrations of NaHS in HepG2 cells counteracts the anti-in ammatory effect at low concentrations In HepG2 cells, high concentrations of NaHS counteracted the anti-in ammatory effect at low concentrations of NaHS [27]. And at lower concentration condition, H 2 S showed positive effects on different diseases, mainly re ecting from in ammatory responses [27; 28; 29].…”

A dual-emission fluorescence-enhanced probe for hydrogen sulfide and its application in biological imaging

“…S9, ESI †). DCH-S is lack of sensitivity to pH with a wide range (2)(3)(4)(5)(6)(7)(8)(9)(10)(11). However, by studying the pH effect, DCH-S was found to respond to NaHS and show obvious fluorescence enhancement in the pH range of 5-9.…”

“…[3][4][5] The endogenously produced H 2 S was found to participate in the regulation of numerous physiological and pathological processes such as neurotransmission, vasodilation, angiogenesis, apoptosis, antioxidation and anti-inflammation. [6][7][8][9][10][11][12] It has also been revealed that abnormal levels of H 2 S is associated with Alzheimer's disease, Down's syndrome, diabetes, and many other diseases. [13][14][15][16][17][18][19] For a better understanding of the complex performance of H 2 S in diverse physiological events as well as pathology of diseases, considerable attention has focused on the accurate and efficient methods to analyze H 2 S in biological systems.…”