Exogenous JH and ecdysteroid applications alter initiation of polydnaviral replication in an endoparasitoid wasp, Cotesia plutellae (Braconidae: Hymenoptera)

Park, Bok Ri; Kim, Yong Gyun

doi:10.5483/bmbrep.2011.44.6.393

Cited by 4 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(B) Quantification of Cp-TSP13 in gene content using qPCR. Hexokinase (HEX) was used as a reference gene [ 79 ]. Different letters above standard deviation bars indicate significant difference among means at Type I error = 0.05 (LSD test).…”

Section: Resultsmentioning

confidence: 99%

Translational Control of Host Gene Expression by a Cys-Motif Protein Encoded in a Bracovirus

Kim

2016

PLoS ONE

View full text Add to dashboard Cite

Translational control is a strategy that various viruses use to manipulate their hosts to suppress acute antiviral response. Polydnaviruses, a group of insect double-stranded DNA viruses symbiotic to some endoparasitoid wasps, are divided into two genera: ichnovirus (IV) and bracovirus (BV). In IV, some Cys-motif genes are known as host translation-inhibitory factors (HTIF). The genome of endoparasitoid wasp Cotesia plutellae contains a Cys-motif gene (Cp-TSP13) homologous to an HTIF known as teratocyte-secretory protein 14 (TSP14) of Microplitis croceipes. Cp-TSP13 consists of 129 amino acid residues with a predicted molecular weight of 13.987 kDa and pI value of 7.928. Genomic DNA region encoding its open reading frame has three introns. Cp-TSP13 possesses six conserved cysteine residues as other Cys-motif genes functioning as HTIF. Cp-TSP13 was expressed in Plutella xylostella larvae parasitized by C. plutellae. C. plutellae bracovirus (CpBV) was purified and injected into non-parasitized P. xylostella that expressed Cp-TSP13. Cp-TSP13 was cloned into a eukaryotic expression vector and used to infect Sf9 cells to transiently express Cp-TSP13. The synthesized Cp-TSP13 protein was detected in culture broth. An overlaying experiment showed that the purified Cp-TSP13 entered hemocytes. It was localized in the cytosol. Recombinant Cp-TSP13 significantly inhibited protein synthesis of secretory proteins when it was added to in vitro cultured fat body. In addition, the recombinant Cp-TSP13 directly inhibited the translation of fat body mRNAs in in vitro translation assay using rabbit reticulocyte lysate. Moreover, the recombinant Cp-TSP13 significantly suppressed cellular immune responses by inhibiting hemocyte-spreading behavior. It also exhibited significant insecticidal activities by both injection and feeding routes. These results indicate that Cp-TSP13 is a viral HTIF.

show abstract

Section: Resultsmentioning

confidence: 99%

Translational Control of Host Gene Expression by a Cys-Motif Protein Encoded in a Bracovirus

Kim

2016

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Knock-down efficacy of RNAi was assessed by RT-PCR of each gene at selected times PI. For control dsRNA, dsRNA specific to a viral gene, CpBV-ORF302, was prepared and similarly injected [47].…”

Section: Methodsmentioning

confidence: 99%

Roles of Peroxinectin in PGE2-Mediated Cellular Immunity in Spodoptera exigua

2014

View full text Add to dashboard Cite

BackgroundProstaglandins (PGs) mediate insect immune responses to infections and invasions. Although the presence of PGs has been confirmed in several insect species, their biosynthesis in insects remains a conundrum because orthologs of the mammalian cyclooxygenases (COXs) have not been found in the known insect genomes. PG-mediated immune reactions have been documented in the beet armyworm, Spodoptera exigua. The purpose of this research is to identify the source of PGs in S. exigua.Principal FindingsPeroxidases (POXs) are a sister group of COX genes. Ten putative POXs (SePOX-A ∼ SePOX-J) were expressed in S. exigua. Expressions of SePOX-F and -H were induced by bacterial challenge and expressed in the hemocytes and the fat body. RNAi of each POX was performed by hemocoelic injection of their specific double-stranded RNAs. dsPOX-F or, separately, dsPOX-H, but not the other eight dsRNA constructs, specifically suppressed hemocyte-spreading behavior and nodule formation; these two reactions were also inhibited by aspirin, a COX inhibitor. PGE2, but not arachidonic acid, treatment rescued the immunosuppression. Sequence analysis indicated that both POX genes were clustered with peroxinectin (Pxt) and their cognate proteins shared some conserved domains corresponding to the Pxt of Drosophila melanogaster.Conclusions SePOX-F and -H are Pxt-like genes associated with PG biosynthesis in S. exigua.

show abstract

“…또한 Mu 등 15) 과 Hwang 16) 은 bisphenol A가 Daphnia magna에서 탈피를 지연시키고 embryo의 발육을 저해하는 anti-ecdysteroidal activity를 가지고 있음을 보고하였고 농업용 fungicides인 fenarimol과 propiconazole이 역시 anti-ecdysteroidal activity를 나 타냄이 보고 17,18) 되었다. 아울러 국내에서도 ecdysone 관련물질들의 생태위해성평가 및 분자생물학적 연구 들 [19][20][21]…”

unclassified

A Comparative Study on the Chemicostructural Characteristics of Ecdysteroids

Hwang¹

2012

Korean Journal of Environmental Health Sciences

View full text Add to dashboard Cite

Objectives: This study was conducted in order to investigate the specific correlation between physicochemical properties and bioactivity in ecdysteroids found in living organisms. Methods: The examined steroidal compounds were classified into three groups according to their relevance to ecdysone activity. Each compound molecule was completely drawn to automatically calculate its physicochemical parameters and docked against 20-hydroxyecdysone to calculate the total distance. Electronic charge distribution was also observed for each molecule. All procedures were conducted using a computational chemistry program. Results: Ecdysone agoinists showed different ranges of parameter values, such as log P, hydrophilic-lipophilic balance (HLB), solubility parameter (SP), hydrophilic surface (HPS), hydrogen bond (HB) and Kappa 2, when compared with antagonists and steroids without ecdysone activity. They also showed a similar electronic charge distribution that is significantly different from the electron charge distribution of antagonists and steroids without ecdysone activity. The total distance values of agonists, estimated by docking them with 20-hydroxyecdysone, were relatively small but showed no correlation with binding affinity with receptor ligand. Conclusions: These results suggest that physicochemical properties such as steric and electronic effects, hydrophobicity and hydrogen bonding may operate in combination to determine the binding activity of ecdysteroids to the receptor protein.

show abstract

Exogenous JH and ecdysteroid applications alter initiation of polydnaviral replication in an endoparasitoid wasp, Cotesia plutellae (Braconidae: Hymenoptera)

Cited by 4 publications

References 41 publications

Translational Control of Host Gene Expression by a Cys-Motif Protein Encoded in a Bracovirus

Translational Control of Host Gene Expression by a Cys-Motif Protein Encoded in a Bracovirus

Roles of Peroxinectin in PGE2-Mediated Cellular Immunity in Spodoptera exigua

A Comparative Study on the Chemicostructural Characteristics of Ecdysteroids

Contact Info

Product

Resources

About