Exogenous melatonin application improves resistance to high manganese stress through regulating reactive oxygen species scavenging and ion homeostasis in tobacco

Gao, Hongsheng; Huang, Lunzeng; Wang, Xueting; Qiao, Xuqiang; Xiao, Fang; Yang, Yuting; Yu, Bohui; Guo, Xiaotong; Yu, Chunyan; Zhang, Hongxia

doi:10.1007/s10725-022-00857-2

Cited by 16 publications

(5 citation statements)

References 65 publications

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“…The MDA level in the MnCl 2 -treated group was 2.91 times that of the control group. Our results concur with the studies showing elevated MDA accumulation in high Mn concentrations in Lepidium sativum 99 , Nicotiana benthamiana 100 and Broussonetia papyrifera 101 . Giannakoula et al 102 suggested that heavy metals could disrupt membrane integrity as well as standard growth in citrus plants.…”

Section: Resultssupporting

confidence: 93%

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Üstündağ,

Macar,

Kalefetoğlu Macar

et al. 2023

Sci Rep

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Although the antioxidant properties of Melissa officinalis extract (Mox) are widely known, little work has focused on its protective capacity against heavy metal stress. The primary objective of this study was to determine the potential of Mox to mitigate manganese (II) chloride (MnCI2)-induced cyto-genotoxicity using the Allium and comet assays. Physiological, genotoxic, biochemical and anatomical parameters as well as the phenolic composition of Mox were examined in Allium cepa (L.). Application of 1000 µM MnCl2 reduced the rooting percentage, root elongation, weight gain, mitotic index and levels of chlorophyll a and chlorophyll b pigments compared to the control group. However, it increased micronuclei formation, chromosomal abnormality frequencies, tail DNA percentage, proline amount, lipid peroxidation level and meristematic damage severity. The activities of superoxide dismutase and catalase also increased. Chromosomal aberrations induced by MnCl2 were fragment, sticky chromosome, vagrant chromosome, unequal distribution of chromatin and bridge. Application of 250 mg/L Mox and 500 mg/L Mox along with MnCl2 significantly alleviated adverse effects dose dependently. The antioxidant activity bestowed by the phenolic compounds in Mox assisted the organism to combat MnCl2 toxicity. Consequently, Mox exerted remarkable protection against MnCl2 toxicity and it needs to be investigated further as a potential therapeutic option.

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Section: Resultssupporting

confidence: 93%

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Üstündağ,

Macar,

Kalefetoğlu Macar

et al. 2023

Sci Rep

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“…The threshold for Mn (Mn 2+ ) toxicity in soybean seedling growth was identified at 0.05 mM. Surpassing this threshold hampers soybean development, primarily by affecting ion absorption and translocation, inciting the generation of ROS, and disrupting hormone homeostasis [32][33][34]. Currently, the specific mechanism governing soybean plants' response to Mn toxicity stress has not been fully elucidated, particularly with regard to the response of old leaves when suffered from Mn poisoning, a subject that has yet to be explored in the literature.…”

Section: Introductionmentioning

confidence: 99%

Regulatory Mechanism through Which Old Soybean Leaves Respond to Mn Toxicity Stress

Pan,

Shi,

et al. 2024

IJMS

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Manganese (Mn) is a heavy metal that can cause excessive Mn poisoning in plants, disrupting microstructural homeostasis and impairing growth and development. However, the specific response mechanisms of leaves to Mn poisoning have not been fully elucidated. This study revealed that Mn poisoning of soybean plants resulted in yellowing of old leaves. Physiological assessments of these old leaves revealed significant increases in the antioxidant enzymes activities (peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT)) and elevated levels of malondialdehyde (MDA), proline, indoleacetic acid (IAA), and salicylic acid (SA), under 100 μM Mn toxicity. Conversely, the levels of abscisic acid (ABA), gibberellin 3 (GA3), and jasmonic acid (JA) significantly decreased. The Mn content in the affected leaves significantly increased, while the levels of Ca, Na, K, and Cu decreased. Transcriptome analysis revealed 2258 differentially expressed genes in the Mn-stressed leaves, 744 of which were upregulated and 1514 were downregulated; these genes included genes associated with ion transporters, hormone synthesis, and various enzymes. Quantitative RT-PCR (qRT-PCR) verification of fifteen genes confirmed altered gene expression in the Mn-stressed leaves. These findings suggest a complex gene regulatory mechanism under Mn toxicity and stress, providing a foundation for further exploration of Mn tolerance-related gene regulatory mechanisms in soybean leaves. Using the methods described above, this study will investigate the molecular mechanism of old soybean leaves’ response to Mn poisoning, identify key genes that play regulatory roles in Mn toxicity stress, and lay the groundwork for cultivating high-quality soybean varieties with Mn toxicity tolerance traits.

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“…When the soluble Mn 2+ concentration exceeded 50 μM, soybean seedling development was inhibited [ 11 ]. Mn stress can affect the absorption and transport of plant ions and lead to the inhibition of root growth, the accumulation of ROS (reactive oxygen species), and the destruction of plant hormone homeostasis in vivo [ 26 , 27 , 28 ]. Therefore, Mn toxicity is one of the main factors influencing soybean root growth and restricting soybean production.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Sequencing Analysis of Root in Soybean Responding to Mn Poisoning

Liu

Pan

et al. 2023

IJMS

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Manganese (Mn) is among one of the essential trace elements for normal plant development; however, excessive Mn can cause plant growth and development to be hindered. Nevertheless, the regulatory mechanisms of plant root response to Mn poisoning remain unclear. In the present study, results revealed that the root growth was inhibited when exposed to Mn poisoning. Physiological results showed that the antioxidase enzyme activities (peroxidase, superoxide dismutase, ascorbate peroxidase, and catalase) and the proline, malondialdehyde, and soluble sugar contents increased significantly under Mn toxicity stress (100 μM Mn), whereas the soluble protein and four hormones’ (indolebutyric acid, abscisic acid, indoleacetic acid, and gibberellic acid 3) contents decreased significantly. In addition, the Mn, Fe, Na, Al, and Se contents in the roots increased significantly, whereas those of Mg, Zn, and K decreased significantly. Furthermore, RNA sequencing (RNA-seq) analysis was used to test the differentially expressed genes (DEGs) of soybean root under Mn poisoning. The results found 45,274 genes in soybean root and 1430 DEGs under Mn concentrations of 5 (normal) and 100 (toxicity) μM. Among these DEGs, 572 were upregulated and 858 were downregulated, indicating that soybean roots may initiate complex molecular regulatory mechanisms on Mn poisoning stress. The results of quantitative RT-PCR indicated that many DEGs were upregulated or downregulated markedly in the roots, suggesting that the regulation of DEGs may be complex. Therefore, the regulatory mechanism of soybean root on Mn toxicity stress is complicated. Present results lay the foundation for further study on the molecular regulation mechanism of function genes involved in regulating Mn tolerance traits in soybean roots.

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Exogenous melatonin application improves resistance to high manganese stress through regulating reactive oxygen species scavenging and ion homeostasis in tobacco

Cited by 16 publications

References 65 publications

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Regulatory Mechanism through Which Old Soybean Leaves Respond to Mn Toxicity Stress

Transcriptome Sequencing Analysis of Root in Soybean Responding to Mn Poisoning

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