Exogenous miRNA: A Perspective Role as Therapeutic in Rheumatoid Arthritis

Saquib, Mohd; Agnihotri, Prachi; Monu,; Biswas, S. N.

doi:10.1007/s11926-021-01009-7

Cited by 21 publications

(20 citation statements)

References 56 publications

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“…Exogenous miRNAs are emerging as a trend for disease treatment because they can be easily obtained from food, occur naturally during the diet, and have fewer deleterious effects. When plant and animal miRNAs are used for a diet, they pass through the mammalian gastrointestinal tract, acting as a direct regulator of the digestive tract and entering the circulation, targeting the appropriate loci to regulate gene expression 34 . However, current studies on the cross-species regulatory effects of exogenous miRNAs are scarce, and there is still huge room for exploration.…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Investigating the miRNA of the Chinese herb Gecko on the cross-species regulation network of hepatocellular carcinoma

Zhao

Tong

et al. 2022

Preprint

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Gecko is a traditional Chinese herb that has been extensively documented as a treatment for various illnesses, including cancer, for thousands of years in ancient Chinese literature. The development of Chinese medicinal preparations and the role of small molecule active ingredients in Chinese medicine have recently emerged as new research hotspots. However, the potential cross-species regulatory mechanisms of microRNAs (miRNAs) from Gecko components in hepatocellular carcinoma (HCC) are not fully understood. In this study, we isolated and extracted total RNA from Chinese herbal Gecko tablets after powdering and screened the Gecko miRNA expression dataset after high-throughput sequencing to compare with the survival-related differentially expressed miRNA dataset in human HCC to identify new Gecko and HCC survival-related miRNA-mRNA regulatory networks. The miR-100-5p, miR-99a-5p, and miR-101-3p were identified as critical miRNAs for the role of Geckos in human HCC. Stepwise prediction and validation, nine downstream mRNAs (EZH2, KPNA2, LMNB1, LRRC1, MRGBP, SMARCD1, STMN1, SUB1, and UBE2A) were found to be determined as key miRNAs interacting with essential genes that interact with each other. A miRNA-mRNA regulatory network consisting of these key genes was constructed, and GO, KEGG enrichment analysis indicated that these key mRNAs might be associated with the suppression and progression of hepatocellular carcinoma. Importantly, immune infiltration analysis showed that the miRNA network was significantly associated with the infiltration abundance of multiple immune cells. These findings suggest that Gecko miRNAs may inhibit HCC progression and treat HCC by targeting critical miRNA-mRNA networks for cross-species regulation. It also provides a reference for future research and new drug development.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Investigating the miRNA of the Chinese herb Gecko on the cross-species regulation network of hepatocellular carcinoma

Zhao

Tong

et al. 2022

Preprint

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“…The downregulation of disease-associated miRNAs can be reversed by artificially synthesized miRNAs to regain the loss of function. The compatibility of exogenous miRNAs with the human body could be utilized as therapeutic options for the treatment of RA to allow the better targeting of specific immunomodulatory proteins that are involved in the RA pathogenesis [128].…”

Section: Discussionmentioning

confidence: 99%

miRNAs as Biomarkers and Possible Therapeutic Strategies in Rheumatoid Arthritis

Kmiołek

Paradowska‐Gorycka

2022

Cells

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Within the past years, more and more attention has been devoted to the epigenetic dysregulation that provides an additional window for understanding the possible mechanisms involved in the pathogenesis of autoimmune rheumatic diseases. Rheumatoid arthritis (RA) is a heterogeneous disease where a specific immunologic and genetic/epigenetic background is responsible for disease manifestations and course. In this field, microRNAs (miRNA; miR) are being identified as key regulators of immune cell development and function. The identification of disease-associated miRNAs will introduce us to the post-genomic era, providing the real probability of manipulating the genetic impact of autoimmune diseases. Thereby, different miRNAs may be good candidates for biomarkers in disease diagnosis, prognosis, treatment and other clinical applications. Here, we outline not only the role of miRNAs in immune and inflammatory responses in RA, but also present miRNAs as diagnostic/prognostic biomarkers. Research into miRNAs is still in its infancy; however, investigation into these novel biomarkers could progress the use of personalized medicine in RA treatment. Finally, we discussed the possibility of miRNA-based therapy in RA patients, which holds promise, given major advances in the therapy of patients with inflammatory arthritis.

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“…Out of several At molecular level there are several biomolecules derived from Brassica oleracea such as exogenous microRNA, contributing to gene regulation of distantly related organisms by increasing their concentrations in blood/plasma/serum or in organs. In-vivo studies reported its regulatory role by increasing the level of Brassica oleracea miRNA [32], indicating that the use of naturally occurring food derived products/regulatory compounds/molecules from Brassica oleracea is beneficial in minimizing the disease symptoms. Therefore, we were interested to identify dietary components from Brassica oleracea with a believe and hope that the identified components may resolve the inflammation occurrence associated with RA without any side effect.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory activity of nicotine isolated from Brassica oleracea in rheumatoid arthritis

et al. 2022

Self Cite

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Rheumatoid arthritis (RA) is an autoimmune disease, associated with chronic inflammation of synoviocytes. TNFα plays a crucial role in the pathogenesis of RA through pro-inflammatory cytokines. Nicotine, an alkaloid used as herbal medicine, often worked as an anti-inflammatory agent. In this study, we tried to uncover the anti-inflammatory impact of nicotine against RA. Nicotine was isolated from Brassica oleracea, purified by high profile liquid chromatography (HPLC). In-silico docking was carried out using bioinformatics tools SWISSADME, PASS, and DIGEP-Pred to determine drug likeliness of nicotine. The In-vitro study was performed in Tumor necrosis factor (TNFα) induced SW982 Synoviocytes by qPCR. mRNA expression of pro-inflammatory cytokines (TNF, IL6, IL1β) and proteins (TRAF2, P50, P65) were analyzed followed by validation of P65 (RELA), pP65, IkBα by western blot analysis. Nicotine compound was extracted from Brassica oleracea and purified by HPLC method (Rt values at 2.67 min). The physicochemical, pharmacokinetic properties and drug-likeliness of nicotine was studied by in-silico analysis. In-vitro studies revealed that nicotine lowers the expression of inflammatory cytokines (TNF, IL6, IL1β) and proteins (TRAF2, P50, P65) at 1 µg/ml in TNFα induced SW982 cells.Nicotine from natural sources (Brassica oleracea) has been found to be an effective anti- inflammatory compound at a low dosage. Thus identified the role of nicotine present in the natural sources as a therapeutic option for RA, may be recommended as remedial drug instead of synthetic drug.

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Exogenous miRNA: A Perspective Role as Therapeutic in Rheumatoid Arthritis

Cited by 21 publications

References 56 publications

WITHDRAWN: Investigating the miRNA of the Chinese herb Gecko on the cross-species regulation network of hepatocellular carcinoma

WITHDRAWN: Investigating the miRNA of the Chinese herb Gecko on the cross-species regulation network of hepatocellular carcinoma

miRNAs as Biomarkers and Possible Therapeutic Strategies in Rheumatoid Arthritis

Anti-inflammatory activity of nicotine isolated from Brassica oleracea in rheumatoid arthritis

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